长期服用珍田胶囊对大鼠组织器官学变化的试验研究

考察大鼠灌胃给予珍田胶囊后组织器官病理学变化。珍田胶囊以1.315、2.63、5.26 g(生药)/kg剂量（分别相当于人临床剂量的13、26.1、52.2倍）,对照组给予相同体积的蒸馏水;连续给大鼠灌胃180 d,逐日观察动物行为、外观及大小便。停药后继续观察4周,检测大鼠脏器系数,观察组织器官病理学变化。各给药组大鼠一般状况未见明显异常,内脏检查无病变。未观察到与受试药物相关的病理组织学改变。     

气相色谱-质谱法测定十味香鹿胶囊挥发油的化学成分

采用气相色谱-质谱联用法对十味香鹿胶囊的挥发油成分进行分析。采用2010年版《中国药典》挥发油测定法对十味香鹿胶囊挥发油进行提取,以毛细管柱进行分析,面积归一化法测定百分含量,并对其化学成分进行鉴定。色谱柱为Agilent19091M-436型弹性石英毛细管色谱柱(0.25mm×30m,0.25μm);程序升温:60℃,以10℃/min升至280℃,保持12min。进样口温度为280℃。质谱条件:EI离子源,电子能量70eV,离子源温度230℃;扫描范围20~500amu。共检出33个化合物,鉴定出其中的22个化学成分,相对含量超过5%的成分有棕榈酸(54.107%)、顺-9-十八烯酸甲酯(8.092%)、反-9-十八碳烯酸(7.285%)、(Z,Z)-9,12-十八碳二烯酸(6.026%)、9-反十六碳烯酸甲酯(5.456%),同时鉴定出具有镇痛作用的丁香酚甲醚(0.172%)。十味香鹿胶囊中挥发油成分主要包含棕榈酸、烯酸以及烯酸甲酯类成分等。     

Characterization of larval instars of artemisia weevil,Adosomus sp.

The artemisia weevil,Adosomus sp.,has become an important wormwood(Artemisia ordosoca and A.sphaerocephala) pest in recent years in Ningxia Province,China.The number of larval instars should be determined before further studies on its life history,biology and integrate management of pest(IMP).In this study,we measured the length and width of head capsule,based on frequency histograms and Dyar's law,from which we concluded that the larvae of Adosomus sp.have four instars.Of the two vari-ables measured,the wi...     

Preliminary study of capsule endoscopy in the small intestine of horses

Objective To evaluate the visibility of various portions of the small intestine in healthy horses using capsule endoscopy. Procedure Six healthy, conscious adult Thoroughbreds were restrained and an endoscopic capsule (PillCam® SB capsule) was inserted into the oesophagus using an intranasal catheter aided by a guide wire. Water (500 mL) flushed the capsule down the gastrointestinal tract. Data were collected and stored in the recorder of the endoscopic system for 6 hours after capsule insertion and the images were evaluated using an image reader and scored using a visual analogue scale. Results Capsule endoscopy enabled observation of the distinct mucosal shape, colour, and villus structure of the intestinal lumen from the duodenum through the proximal jejunum. At 4 h after passing the pylorus, the endoscopic capsule started transmitting increasingly dark images in the distal jejunum as the lumen circumference increased. Means of the visual analogue scale in the duodenum, proximal jejunum, and distal jejunum were 93.8 ± 1.3%, 86.2 ± 2.5% and 48.8 ± 6.3%, respectively. Differences among these values were statistically significant (P < 0.05). Conclusions and Clinical Relevance Capsule endoscopy enables observation of the distinct mucosal shape, colour and villus structure of the proximal and mid-small intestine in healthy horses.     

The use of preserved equine renal capsule to repair lamellar corneal lesions in normal dogs

The purpose of this study was to evaluate the use of equine renal capsule preserved in 98% glycerine to repair lamellar corneal lesions in normal dogs. For this purpose, 12 dogs, divided into six groups ( n  = 2), were used to evaluate the 1st to 7th day, 15th day and 30th to 60th postoperative day. In order to perform the histologic study, the clinical procedures were analyzed, while the recipient's corneas were collected. The photophobia and blepharospasm also were more intense in the 1st to 7th postoperative day, and regressed in the 15th postoperative day. Therefore, the edema and the vascular events were both more frequent in the intermediary phases and regressed in the late periods. On the other hand, the morphological evaluation demonstrated an inflamatory exudate, also in the intermediary and late periods. These results suggested that the equine renal preserved capsule could be a useful alternative tissue to repair lamellar corneal lesions in dogs.     

醋柳红素胶囊对老龄小鼠的抗氧化作用

本文研究醋柳红素胶囊对老龄小鼠的抗氧化作用。给予老龄小鼠不同剂量的醋柳红素胶囊内容物灌胃,连续30d,检测小鼠血清中超氧化物歧化酶（SOD）活性,全血谷胱甘肽过氧化物酶（GSH—PX）活性和过氧化脂质（MDA）的含量。结果表明醋柳红素胶囊无提高小鼠红细胞SOD活性,能提高GSH—PX活性,显著降低小鼠血中MDA含量,结果显示醋柳红素胶囊具有抗氧化作用。     

搓压式蓖麻脱壳机脱壳过程运动分析与试验

为优化蓖麻蒴果柔性脱壳装置的脱壳过程参数,基于离散元粘结接触理论,构建蓖麻蒴果脱壳过程仿真模型,对物料在脱壳室内运动过程进行分析。结果表明,当滚筒转速为250r/min、脱壳间隙为7mm、填充率为40%时,脱壳率最高。滚筒转速对颗粒受到的最大压力和最大速度影响显著,随着转速的增加,颗粒间最大挤压力从68.78N减小到68.10N,颗粒最大速度从8.92m/s增加到12.99m/s,脱壳率从91.23%增加到91.28%,然后下粒最大压力从76.93N下降到58.69N,颗粒最大速度先由12.14m/s增加至12.99m/s,后减小至10.05 m/s;脱壳率先由92.50%减小至89.59%,后增加至91.41%。蓖麻蒴果在脱壳过程中,颗粒在X轴方向运动的平均速度从4.17m/s减小到3.26m/s;颗粒在Y轴方向运动的平均速度从8.26m/s减小到7.59m/s;颗粒在Z轴方向运动的平均速度从6.58m/s减小到6.24m/s。开始脱壳阶段,蓖麻蒴果集中分布在脱壳室中部,部分呈堆积现象,其运动轨迹为接触内滚筒后弹起一定高度后下落并随内滚筒转动,向出料口方向运动;稳定脱壳阶段,蓖麻蒴果集中均匀分布在脱壳室出料口附近,并随着内滚筒一起转动;脱壳结束阶段,物料集中分布在脱壳室出料口底部位置,并不断向底部位置移动,大部分蓖麻从脱壳室右侧排出。通过仿真得到脱壳过程最优参数组合为：转速350r/min、脱壳间隙5mm、填充率40%。试验结果为：转速350r/min、脱壳间隙5mm、填充率30%,因3个因素中转速和脱壳间隙为极显著因素,填充率为显著因素,故差异在合理范围内。     

基于卷积胶囊网络的百合病害识别研究

为了提高百合病害诊断模型的抗噪能力,以VGG-16模型为基础构建卷积胶囊网络,并分析了胶囊尺寸、路由迭代次数对训练时间及模型精度的影响。最终得到胶囊尺寸为8、路由迭代次数为3的卷积胶囊网络,该网络对百合病害诊断精度达到99.20%。使用不同等级的高斯噪声、椒盐噪声、斑点噪声、仿射变换图像对模型抗噪能力进行测试,结果表明,卷积胶囊网络明显优于VGG-16模型,更适合在实际生产环境下的百合病害诊断。     

香型多花蝴蝶兰杂交及优良单株选育

【目的】利用收集的蝴蝶兰种质资源开展香型多花蝴蝶兰的杂交选育,观察后代群体主要目标性状表现,获得符合目标性状要求的香型多花蝴蝶兰优良单株。【方法】以15个蝴蝶兰品种为亲本开展蝴蝶兰常规杂交,对杂交后代进行性状调查分析,并与亲本进行比较。【结果】配对的15个杂交组合中编号16161(PL608×PL321)和编号16162(390×PL321)两对组合成功,在2016年获得成熟蒴果,其他13对杂交组合均没有获得蒴果或成熟蒴果。亲本性状最相近的16161栽培杂交后代群体200株,平地空调催花174株,该杂交组合后代数量多、开花株多、株叶型整齐、生长势强,完全遗传了母本的香味;花朵数量均遗传母本特性,接近母本,多于父本;花色、唇色整体上为父母本花色的调和色,有深浅梯度变化;斑点分布规律同母本,未出现亲本花色之外的花色,符合基本遗传规律。部分杂交后代生长发育指标优于双亲,选育出有香味、花朵数量多、花色鲜亮、开花株形态好的优良单株6株。16162栽培杂交后代群体21株,通过平地空调催花12株,杂交后代均没有遗传亲本的香味,花朵数量多遗传自父本,未选育出香型多花优良单株。【结论】两个亲本相近的香型蝴蝶兰通过杂交,可选育出符合目标性状的香型多花蝴蝶兰优良单株。但由于蝴蝶兰香味遗传背景及机制复杂,在亲本均有香味的情况下,后代不一定能遗传亲本的香味。试验中两个香型蝴蝶兰PL608、PL321可为今后香型多花蝴蝶兰杂交选育亲本选配提供参考。     

组方优化的阳和胶囊对木瓜蛋白酶诱导大鼠骨关节炎的作用研究

目的探讨组方优化的阳和胶囊对木瓜蛋白酶诱导的大鼠骨关节炎的治疗作用及其作用机制。方法采用大鼠膝关节腔内注射10%木瓜蛋白酶和0.03mol/L L-半胱氨酸混合溶液建立大鼠骨关节炎模型。于造模后分别灌胃给予组方优化的阳和胶囊高、中、低剂量及阳性药盐酸氨基葡萄糖(0.13g/kg),每日1次,连续4周。实验期间观察大鼠一般状态,每周测定大鼠膝关节肿胀度。实验结束后测定大鼠膝关节灌洗液中IL-1β、IL-6、TNF-ɑ含量,及关节滑膜组织中糖胺多糖(GAGs)含量。结果实验期间模型组大鼠膝关节显著肿胀(P<0.01),各给药组与模型组相比均有不同程度缓解。组方优化的阳和胶囊高、中剂量组能够显著降低膝关节灌洗液中IL-1β、IL-6、TNF-ɑ的含量(P<0.01),显著增加关节滑膜组织中GAGs含量(P<0.01)。结论组方优化的阳和胶囊对木瓜蛋白酶诱导的大鼠骨关节炎具有治疗作用,其机制可能与降低膝关节灌洗液中IL-1β、IL-6、TNF-ɑ的水平和增加关节滑膜组织中GAGs的含量有关。