高效液相色谱法测定复方二仙草胶囊中淫羊藿苷含量

目的：建立HPLC测定复方二仙草胶囊中淫羊藿苷含量的方法。方法：Shim-pack VP-ODS C18分析色谱柱（4.6 mm×150 mm,5μm）,Phenomenex ODS C18保护柱（3 mm×4 mm）。流动相：乙腈-水（26∶74）,流速1.0 mL/min,检测波长270 nm。结果：淫羊藿苷保留时间为13.8 min。以峰面积Y对进样量X（μg）线性回归,淫羊藿苷回归方程：y=2 732.7x＋12.860r,=1.000 0,线性范围0.1-1.5μg。淫羊藿苷回收率为102.16%,RSD为1.92%。结论：本方法操作简便,结果准确可靠,可用于复方二仙草胶囊中淫羊藿苷的含量测定。     

Traumatic corneal laceration with associated lens capsule disruption: a retrospective study of 77 clinical cases from 1999 to 2009

Objective Examine prognostic factors that may indicate when surgical lens removal is indicated to prevent vision‐threatening complications in patients presented following traumatic perforating corneal laceration with associated lens capsule disruption. Procedures Seventy‐seven patients (10 cats and 67 dogs) were evaluated with this injury; of these, 47 were presented acutely and treated surgically and/or medically. Successful outcome was defined as functional vision in the treated eye. Results The 47 patients with acute injuries were divided into 3 treatment groups A‐C for comparison; A ‐ corneal repair/lens removal (n = 15), B ‐ corneal repair/no lens removal (n = 9), C – medical management (n = 23). Groups A and B showed a significantly greater rate of vision loss compared to Group C that was most obvious greater than 18 months post‐injury (P = 0.029 and 0.0097, respectively). Cox proportional hazards regression analysis found a significantly higher increased rate of vision loss in Group A (HR = 4.5; P = 0.023) and a higher but nonsignificant increased rate of vision loss in Group B (HR = 3.0; P = 0.23) compared to Group C after controlling for age and cause of injury. The length of the corneal laceration and time interval from injury to referral were also relevant prognostic factors. Conclusions Medical management is an appropriate therapy for patients of all ages suffering perforating corneal injuries with associated lens capsule disruption. Patients with corneal injuries requiring surgical repair or managed by lens removal following corneal repair suffered vision‐threatening complications approximately 3 to 4.5 times the rate of patients treated by medical management, respectively. Favorable prognostic signs for treatment by medical management include good corneal wound apposition and a formed anterior chamber without uveal prolapse or continued aqueous leakage.     

Effects of Qili Qiangxin capsule on serum concentration of adiponectin and heart function in patients with coronary heart disease combined with congestive heart failure

AIM：To investigate the effects of Qili Qiangxin capsule on serum adiponectin (APN), serum N-terminal pro-brain natriuretic peptide (NT-proBNP) and heart function in the patients of coronary heart disease combined with congestive heart failure. METHODS：One hundred and twenty patients were randomly divided into treatment group and control group, and both groups were given anti-failure routine therapy. The patients in treatment group were treated with Qili Qiangxin capsule and the patients in control group were treated with placebo. The patients in the 2 groups were given a certain dose of the drugs for 6 months. The New York Heart Association (NYHA) heart function classification, left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD),left ventricular ejection fraction (LVEF), and 6-min walking test (6-MWT) were observed before and after treatment. The levels of APN, NT-proBNP were measured by ELISA before and after treatment. RESULTS：With the increase in the class of NYHA heart function, the serum concentrations of APN and NT-proBNP in the heart failure cases increased significantly. After 6-month treatment, the effective rate in experimental group was 91.7% and that in control group was 75.0%. A significant difference was found between the 2 groups (P<0.01). After treatment, LVEDD and LVESD in both groups were decreased significantly, and LVEF in both groups was increased significantly. The serum concentrations of APN and NT-proBNP decreased significantly (P<0.05). 6-MWT result was improved significantly. Compared with control group, more obvious effect was observed in experimental group (P<0.05). CONCLUSION：Treatment with Qili Qiangxin capsule reduces the levels of APN and NT-proBNP in the patients with coronary heart disease combined with congestive heart failure.     

Inhibitory effect of Shenci capsule in combination with DDP on angiogenesis in mice with Lewis lung cancer

AIM: To investigate the inhibitory effect of Shenci capsule, a Chinese medicine, in combination with cisplatin (DDP) on the tumor growth and angiogenesis in mouse Lewis lung cancer.METHODS: Forty mice with Lewis lung cancer were randomly divided into 4 groups: Shenci capsule group, Shenci capsule in combination with DDP group, DDP group and control group. Shenci capsule, Shenci capsule in combination with DDP,DDP and sodium chloride wene given, respectively. After 21 days, the weight of subcutaneous tumors was measured, and the tumor inhibition rate was calculated. The microvessel density (MVD) and the protein level of vascular endothelial growth factor (VEGF) were detected by immunohistochemical(IHC) staining. The mRNA expression levels of kinase insert domain-containing receptor (KDR) and the receptor of VEGF were detected by FQ-PCR. RESULTS: The tumor growth inhibitory rate in Shenci capsule in combination with DDP group (63.99%) was higher than that in Shenci capsule group, DDP group and control group (P<0.01). The tumor MVD, VEGF protein and KDR mRNA expression level in Shenci capsule in combination with DDP group were lower than those in the above three groups (P<0.01). CONCLUSION: Shenci capsule in combination with DDP have addition or synergistic effects to inhibit the tumor growth and angiogenesis in mouse Lewis lung cancer. Down-regulation of VEGF and its receptor KDR may be one of the mechanisms that combined use of these two drugs for inhibiting angiogenesis.     

金耳糖肽胶囊的Ⅱ期临床观察

使用金耳糖肽胶囊治疗慢性乙型肝炎 10 0例 ,显效 38例 ,有效 5 0例 ,总有效率 88% ,与二个对照相比 ,差异显著 (P <0 0 5 ) ,对受试者临床症状、体征、实验室指标改善的疗效明显优于对照。对HBV—M清除与对照相比 ,效果不显著 ,可能与疗程短 ,用药量不足有关。