水牛呼吸道杯状细胞分泌糖蛋白的化学特性研究

水牛气管和支气管杯状细胞中的分泌糖蛋白糖链含末端α-N-乙酰基-D-半乳糖胺、α-L-岩藻糖、N-乙酰基-D-葡萄糖胺和唾液酸。根据糖链末端糖基的种类,水牛杯状细胞的分泌糖蛋白的性质与人呼吸道杯状细胞相近似,而与猕猴气管杯状细胞差别甚大。另外,水牛呼吸道的杯状细胞间存在化学组成差别,不同杯状细胞的分泌糖蛋白中含不等量的同一末端糖基或缺乏这一末端糖基。     

Preliminary observations on plasma fibrinogen and plasma protein concentrations and on plasma protein: Fibrinogen ratios in clinically healthy buffaloes

The plasma fibrinogen concentration, the total plasma protein concentration and the plasma protein to fibrinogen ratio (PP:F) were determined in clinically healthy Nili Ravi buffaloes. The plasma fibrinogen concentrations in calves, lactating and non-lactating buffaloes were 513±62, 615±90 and 544±74 mg/dl, respectively, and were statistically different (p<0.05). Total plasma protein concentrations in these animals were 7.15±0.28, 9.32±0.53 and 8.79±0.58 g/dl. PP:F for all animals was between 11 and 19. Fibrinogen levels were positively correlated with plasma protein (r=0.59) and negatively correlated with PP:F (r=–0.59).Abbreviations PP:F plasma protein to fibrinogen ratio     

泌乳水牛日粮能量转化的研究

应用消化代谢实验对 6头泌乳前期的三品杂水牛日粮能量在体内的转化进行研究。结果表明 :①泌乳水牛能量消化率为 6 7 2 3% ,总能代谢率 (ME/GE)为 5 4 0 1% ,消化能代谢率 (ME/DE)为 80 2 4 % ,水牛产奶对代谢能的利用率为 36 85 % ;②可用采食总能的 10 %估算甲烷能的损失 ;③体增热为 339 5 9kJ/ (W0 75·d)。     

注入嵌合法制作黄牛和水牛种间嵌合胚的初步研究

采用注入嵌合法初步建立了一套黄牛和水牛种间嵌合的程序与方法。采用机械剥离法或免疫外科法分离胚胎内细胞团(ICM),然后注入到已去除ICM的受体囊胚中构建形成水牛和黄牛的嵌合胚。结果发现,在用免疫外科法分离ICM时,抗血清的灭活温度从57℃升至63.5℃,ICM的获得数显著升高(0%vs100%,P<0.01),如若在分离培养液中添加6%的胎牛血清(FCS),ICM的获得数大大降低(97.6%vs0%,P<0.01)。采用免疫外科法分离得到的黄牛ICM进行水牛囊胚的ICM置换重组,重组胚的存活率与机械剥离法得到的ICM无显著差异(91.4%vs87.5%,P>0.05);但囊胚孵化率则显著提高(80%vs43.8%;P<0.05)。以上结果表明,⑴水牛和黄牛胚胎通过ICM置换获得的种间嵌合胚胎能继续发育;⑵用于黄牛ICM分离的兔抗牛抗血清需在63.5℃灭活30min,且分离需在无血清的培养液中进行;⑶通过分离ICM置换进行胚胎嵌合时,免疫外科法优于显微手术法。     

摩本杂F1水牛生长性能研究

对摩本杂F₁水牛在昆明小哨的全放牧条件下3年的生长性能进行研究，结果表明：初生体重平均36.40 kg，成年牛平均体重558.90 kg。     

Comparison of voluntary feed intake, rumen passage and degradation kinetics between crossbred Brahmam cattle (Bos indicus) and swamp buffaloes (Bubalus bubalis) fed a fattening diet based on corn silage

Three crossbred tropical cattle and three crossbred buffaloes, fitted with rumen cannulas, were used to investigate the differences in feed intake, digestibility and parameters with rumen degradation kinetics between cattle and buffalo fed a fattening diet in the Philippines. The animals were fed a diet consisting of 50% corn silage (CS), 30% brewer's grain (BG) and 20% concentrate mixture (CM) on a dry matter (DM) basis, at a level of 3% bodyweight (BW) as DM. Total DM intake (DMI) and rumen fill were greater (P < 0.05) for buffaloes than for cattle, but significant differences were not detected between the animal species when those were expressed as percentage of BW. The DMI per metabolic body size for BG and CM were greater for buffaloes than for cattle (P < 0.05), whereas that of CS did not differ between the species (P > 0.05). The weight proportion of consumed feedstuffs differed between cattle and buffaloes (P < 0.05), although nutrient intake per total DMI did not differ significantly (P > 0.05) between the animal species. Nutrients digestibility was higher (P < 0.05) for buffaloes than for cattle. Ruminal passage rate constant of feed particles and rumen fluid did not differ (P > 0.05) between the animal species. Higher DM degradation rate constant and effective degradability with CS were prominent for buffaloes than for cattle (P < 0.05). Degradation parameters did not differ (P > 0.05) between cattle and buffaloes, neither with BG nor CM. The results indicate that the greater digestibility with DM and energy for buffaloes are ascribable to greater effective degradability of CS fed ad libitum level.     

FSH受体基因单核苷酸多态性与卵巢功能

\t\t\t\t\t目的\t\t\t\t\t揭示槟榔江水牛FSHR基因的结构与功能。\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t方法\t\t\t\t\t采用RT-PCR法克隆了槟榔江水牛FSHR基因的编码区全序列,并利用生物信息学方法对其基因编码产物的理化特性、结构及功能进行了初步分析。\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t结果\t\t\t\t\t槟榔江水牛FSHR基因编码区全长为2 088 bp,编码695个氨基酸。氨基酸序列比对显示：槟榔江水牛FSHR与其他哺乳动物的同源性在89.4%以上。槟榔江水牛FSHR蛋白N-端含信号肽和7个跨膜结构,属细胞膜疏水蛋白。该蛋白含有7tmA_FSH-R、LRRNT、LRR重复单元和GnHR_trans等4个保守结构域。槟榔江水牛FSHR二级结构主要由α螺旋和无规则卷曲所构成,分别占41.73%和38.71%。FSHR最有可能在细胞的转运和结合过程中发挥功能作用(概率\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tPurposes\t\t\t\t\tIn order to reveal the structure and fuction of Binglangjiang buffalo FSHR gene.\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tMethods\t\t\t\t\tThe whole coding sequence (CDS) of the FSHR gene was cloned using RT-PCR method, and the physicochemical properties, structure and fuction of its coding product were analysed preliminarily based on the method of bioinformatics.\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tResults\t\t\t\t\tThe completed CDS of Binglangjiang buffalo FSHR gene is 2 088 bp in length, encoding a peptide composed of 695 amino acid residues. The amino acid sequence alignments showed that identity is more than 89.4% between Binglangjiang buffalo and other mammals. Binglangjiang buffalo FSHR protein is a hydrophobic protein with one N-terminal signal peptide and seven transmembrane regions, located in cell membrane. This protein contains 7tmA_FSH-R, LRRNT, LRR and GnHR_trans conservative domains. The secondary structures of buffalo FSHR are mainly consist of alpha helix and random curl, occupied 41.73% and 38.71%, respectively. The buffalo FSHR probably function in the process of cell transport and binding (probability\t\t\t\t\t\t\t\t\t\t\t\t\t0 05), and the cleavage rate was 57 5% and 49 0% respectively ( P> 0.05). These results indicate that buffalo oocytes can be matured in vitro for either 24 h or 30 h under the present experimental conditions.