番茄单性结实研究进展

对番茄单性结实资源、评价鉴定方法、子房内源激素水平、分子机制及新品种选育等的研究现状进行了综述,尤其对近几年分子水平的最新研究进行了评述,对今后这一领域的研究做了展望。     

Metabolie Engineering of Flower Color in Ornamental Plants

Abstract

Advances in the knowledge of plant pigment pathways at genetic, biochemical and molecular levels, and the establishment of genetic transformation methods for an increasing number of plant species have paved the way to genetic engineering of flower and plant color for ornamental purposes. From trial-and-error approaches based on the available few genes, the trend is now to comprehensively study the target species, genotypes and pigment pathways to precisely address specific breeding goals. This review examines the state of the art in this field, from the pioneering stages to fundamental and applied research on flavonoids, which is being extended to carotenoids and is also likely to involve other pigments in the near future.     

塔里木河中下游生态养羊技术模式探讨--以尉犁县为例

生态养殖是畜牧业可持续发展的重要组成部分,生态养羊是针对塔里木河中下游地区脆弱的环境条件,为保护和恢复退化生态系统平衡,发展区域经济提出的生态可持续发展方式。针对塔里木河中下游地区的实际情况,依据退化生态系统恢复、产业生态学、系统的能流功能原理、可持续发展原则、景观生态学的理论,对生态养羊中的退耕还草、还牧,天然草地的恢复,圈舍建设,繁殖饲养管理,粪便的无害化生态处理等进行分析,提出生态养羊的相应理论框架。     

Bread-Making Quality Indices in Triticum aestivum Progenies. Implications in Breeding for Better Bread Wheat

Bread-making quality indices (dough strength and dough mixing stability) in relation to flour protein content, glutenin/gliadin ratio, and high-molecular-weight (HMW) subunits of glutenin have been investigated in Triticum aestivum progenies during a three year agronomic trial. Dough strength (W) proved to be a fairly stable characteristic, slightly but positively correlated with flour protein content. High could be associated with a high glutenin/gliadin ratio as well as with the presence of specific HMW. subunits of glutenin, while high protein content tended to favour a balanced dough tenacity-extensibility ratio (P/L = 0.4—0.6). Satisfactory values of dough-mixing stability were frequently observed in association with good expression of W showing that the two quality traits may coexist without much difficulty in the same genotype. From the plant breeding standpoint the data suggest feasible to obtain high dough strength by concentrating in a genotype the HMW subunits of glutenin known to have a beneficial effect on W. However, very high W may present unfavourable P/L ratios. This possibility is enhanced when the flour has a low protein content which often occurs in high yielding genotypes.     

优质抗白粉病网纹甜瓜新品系的选育及利用

利用抗病的哈密瓜类型材料与网纹类型材料杂交 ,结合田间自然发病和苗期人工接种抗性鉴定 ,通过连续自交分离选择 ,获得 3个稳定的抗白粉病的网纹类型品系材料。经果实性状调查和杂交组合配制 ,3个品系均表现出网纹形成良好、果肉厚 ,折光糖含量高等特点 ,为网纹甜瓜新品种选育提供了亲本材料     

DNA去甲基化参与提高白菜型冬油菜抗寒性的生理机制

利用来自不同育种环境的10份白菜型冬油菜材料,通过半致死温度的测定分析其抗寒性与环境的关系,并利用不同浓度DNA甲基化抑制剂5-azaC处理,分析DNA去甲基化对白菜型冬油菜DNA整体甲基化水平及低温胁迫下苗期生理特性的影响。结果表明,不同育种环境选育的材料,其半致死温度具有显著差异,其中材料2018-FJT、DT-7、DT-9与MXW-1的半致死温度分别为-16.04℃、-15.98℃、-15.63℃、-15.04℃;材料CT-2360、CT-2380、CT-2400、CT-2420、CT-2440、CT-2460的半致死温度分别为-11.32℃、-11.6℃、-11.42℃、-11.44℃、-12.97℃、-13.28℃。依据半致死温度,10个白菜型冬油菜抗寒性由强到弱依次为:2018-FJTDT-7DT-9MXW-1CT-2460CT-2400CT-2380CT-2420CT-2440CT-2360。抗寒性的形成与育成环境的纬度和年平均气温呈极显著正相关。选择抗寒性及产地不同的4个材料CT-2360、MXW-1、2018-FJT、DT-7进行生理测定,结果表明,1 000μmol·L~(-1) 5-azaC处理能显著抑制幼苗的根生长,4个材料的根长较对照减少93.14%～95.06%;低温下5-azaC处理对抗寒性较弱材料CT-2360幼苗的相对电导率、丙二醛含量以及强抗寒性材料DT-7幼苗的脯氨酸、可溶性蛋白含量影响最显著(P0.05);低温处理过程中,4个材料的SOD、POD、CAT活性均上升,以低温处理5d最显著(P0.05),其中2018-FJT的SOD活性增幅最高,为45.85%,DT-7的POD活性增幅最大,为460%,CT-2360的CAT活性增幅最显著,为321.02%。HPLC分析发现常温下抗寒性较弱材料CT-2360的甲基化水平为77.48%,高于其他3个抗寒性强的材料;经过5-azaC处理后发生明显的去甲基化作用,证明抗寒能力受DNA去甲基化的调控。     

棉枯萎抗性定向培育对棉籽球蛋白与棉紫素的影响

研究了同源的抗枯萎病品种西农87岱16抗和感枯萎病品种原岱16棉花种子的生化成分及其性质差异.结果表明,抗病性不同的两类品种的种子球蛋白的种类,不同分子量球蛋白的合成比率及次生代谢物棉紫素的含量都有较大变化.本项研究结果支持定向培育法是一种科学的棉花抗病育种方法.     

Genetic mapping,marker assisted selection and allelic relationships for the Pu6 gene conferring rust resistance in sunflower

Rust resistance in the sunflower line P386 is controlled by Pu₆, a gene which was reported to segregate independently from other rust resistant genes, such as R₄. The objectives of this work were to map Pu₆, to provide and validate molecular tools for its identification, and to determine the linkage relationship of Pu₆ and R₄. Genetic mapping of Pu₆ with six markers covered 24.8 cM of genetic distance on the lower end of linkage Group 13 of the sunflower consensus map. The marker most closely linked to Pu₆ was ORS316 at 2.5 cM in the distal position. ORS316 presented five alleles when was assayed with a representative set of resistant and susceptible lines. Allelism test between Pu₆ and R₄ indicated that both genes are linked at a genetic distance of 6.25 cM. This is the first confirmation based on an allelism test that at least two members of the R_adv/R₄/R₁₁/ R_13a/R_13b/Pu₆ cluster of genes are at different loci. A fine elucidation of the architecture of this complex locus will allow designing and constructing completely new genomic regions combining genes from different resistant sources and the elimination of the linkage drag around each resistant gene.     

杂交水稻高产栽培育苗技术

水稻是贵州省遵义地区赤水市主要粮食作物,在粮食生产中占有举足轻重的地位。目前,种植品种基本是杂交水稻。培育壮苗是杂交水稻生产的关键,秧苗素质的好坏决定着杂交水稻的增产潜力。     

跨区域产学研合作可以促进农作物育种科技创新收敛吗?——基于创新链视角的研究

为促进我国种业科技创新能力的提升,本研究运用全国31个省(市、自治区) 1999-2020年的面板数据(统计数据未含港澳台地区,下同),基于创新链视角,将农作物育种科技创新划分为基础研究、应用研究及试验发展阶段,采用社会网络分析方法和Dagum基尼系数分别对跨区域产学研合作的发展特征及育种科技创新的地区差异进行分析、运用β收敛模型检验跨区域产学研合作对农作物育种科技创新收敛的影响。研究表明：1)跨区域产学研合作呈现增长趋势,但合作中创新资源传递的效率降低。2)农作物育种科技创新产出存在较大的地区差异,但差异逐渐缩小,地区间差异是造成地区总体差异的主要原因。相对于试验发展阶段,基础研究和应用研究创新产出的地区差异较大、差异缩小的速度较慢。3)农作物育种科技创新存在收敛性发展特征,跨区域产学研合作加速了其创新收敛。从全国层面看,跨区域产学研合作对基础研究创新收敛的促进作用最强,对应用研究的促进作用最小。分区域看,跨区域产学研合作促进了东、中、西部地区的创新收敛,且对中、西部创新产出增长的促进作用更大。从时间演变看,由于合作中创新资源传递的效率降低,跨区域产学研合作对农作物育种科技创新收敛的...