Effect of Luteolin on Blood Indexes,Liver and Kidney in Mice with Acute Mercury Poisoning

To study the effect of luteolin on blood indexes,liver and kidney in mice with acute mercury poisoning,28 mice were randomly divided into four groups:Control group (intraperitoneal injection 0.9% saline),luteolin group (lavage 100 mg/kg luteolin),mercuric chloride group (intraperitoneal injection 4 mg/kg mercury chloride) and mercury chloride+luteolin group (intraperitoneal injection 4 mg/kg mercuric chloride,lavage 100 mg/kg luteolin).The activities of ALT in serum,AST,CREA and BUN contents,blood WBC,RBC,HGB content and GSH and MDA contents of liver tissue were detected.Morphological changes of liver and kidney tissues were observed.The results showed that compared with the control group,the activities of ALT and AST of mercuric chloride group were extremely significantly increased (P < 0.01),serum CREA,BUN,blood WBC and liver tissue MDA contents were significantly increased (P < 0.05) while blood RBC,HGB and liver tissue GSH contents were significantly decreased (P < 0.05).Liver,kidney pathological changes were obviously.Compared with mercuric chloride group,the activities of ALT,AST in serum,CREA,BUN,blood WBC and liver tissue MDA contents of mercuric chloride+luteolin group were significantly decreased (P < 0.05),while blood RBC,HGB and liver tissue GSH contents were significantly elevated (P < 0.05).Liver,kidney pathological changes were attenuated obviously.The poisoning were characterized by inflammation and the occurrence of anemia when acute mercury poisoning occurred,liver and kidney showed different degrees of injury in mice.Luteolin could reduce the toxic effects of acute mercury poisoning on blood,liver and kidney.     

猪瘟免疫猪接种PRRSV Nsp2Δ1882-2241弱毒疫苗后免疫应答及T细胞表型变化特征

选择17头28日龄的CSFV和PRRSV抗体均为阴性的仔猪,于试验的第1天和第14天分别对其进行猪瘟耐热保护剂活疫苗（兔源）和高致病性猪繁殖与呼吸综合征Nsp2A1882—2241弱毒疫苗免疫。在免疫后的第28、42天采集外周血液,分析特异性抗体表达量和外周血T淋巴细胞表型的变化,评估猪瘟免疫对猪繁殖与呼吸综合征免疫的影响。结果显示,在CSF免疫后第28、42天,CSFV高抗组中的CD4^＋、CD4^＋／CD8^＋、CD4^＋CD8^＋和CD4-CD8数目均比CSFV低抗组高,CD3^＋和CD8^＋细胞数量比CSFV低抗组低;PRRS高抗组中,CD4CD8-细胞含量高于PRRS低抗组;在CSF免疫后第28天,CSFV抗体产生较高（阳性比率为73．33％）,PRRSV抗体产生较低（阳性比率仅为6．67％）。在CSF免疫后的第42天,CSF高抗组中PRRSV抗体阳性比率较CSF低抗组高8．33％。结果表明,CSFV特异性抗体产生高时能增加PRRSV特异性细胞免疫应答,增加CD4^＋细胞、CD4^＋CD8^＋细胞数量,提高机体免疫水平。CD3＋和CD4CD8-细胞应答作用值得重视。     

伊犁马1000m速步训练赛各阶段静脉血中血气指标变化研究

利用i-STAT血气分析仪检测12匹伊犁马1000 m速步训练赛静息、热身后及赛后即刻、15 min、30 min、60 min时静脉血中的血气指标,并对各血气指标的差异性和相关性进行统计分析。结果表明,运动前后静脉血中PvCO₂、PvO₂、HCO₃、TCO₂、SvO₂和Glu变化差异不显著(P>0.05)。血液pH、BE和Ca²⁺在赛后即刻均明显降低,但经15 min休息后都基本回升到静息水平;K⁺、Hct和Hb在赛后即刻均显著增加(P<0.05),但经15 min休息后都基本回降到静息水平。血液pH与BE、HCO₃呈极显著正相关(P<0.01),与TCO₂浓度呈显著正相关(P<0.05),Hct、Hb与pH和HCO₃呈极显著负相关(P<0.01),与K⁺呈极显著正相关(P<0.01)。因此,BE、HCO₃、TCO₂、Hct和Hb对酸碱平衡均有一定的调节作用;Na⁺、K⁺和Ca²⁺对机体运动具有一定的调节作用。     

抗感败毒口服液对大鼠生长和血液理化指标的影响

为了评价抗感败毒口服液临床用药的安全性,对SD大鼠的生长和血液理化指标进行了试验研究,选用了健康大鼠80只,雌雄各半,随机分为高、中、低剂量组和对照组,其中高剂量组、中剂量组和低剂量组分别灌胃给予该口服液8 mL/kg、4 mL/kg和2 mL/kg体重,对照组按8 mL/kg体重灌服生理盐水,每天一次,连续给药35 d。各组末次给药24 h后,检测大鼠体重、血常规指标、血液生化指标、脏器系数及组织病理变化情况。结果表明,抗感败毒口服液各剂量组大鼠的体重、血液学检查、血液生化指标检查与对照组比较,均无显著差异（P>0.05）,雌性大鼠高剂量组的肝脏脏器指数与对照组相比有显著差异（P<0.05）,其他无显著差异（P>0.05）。肉眼观察实质器官无异常病变。结果提示,长期应用抗感败毒口服液对大鼠生长和血液理化指标无显著影响,临床用药安全。     

Effect of oxytocin infusion on luteal blood flow and progesterone secretion in dairy cattle

The objective of this study was to investigate the effects of oxytocin infusion on corpus luteum (CL) function during early to mid-diestrus by measuring luteal size (LS) and luteal blood flow (LBF) along with plasma levels of progesterone (P4) and prostaglandin metabolites (13,14-dihydro-15-keto-prostaglandin F_2α, PGFM). On day (D) 7 of the estrus cycle (D1 = ovulation), seven cows received 100 IU of oxytocin (OXY) or placebo (PL) following a Latin square design. LS and LBF increased in both groups over time and no differences were observed between the groups. PGFM did not differ either within the groups over time or between the groups at any time point. P4 of the OXY group was higher compared to that of the the PL group 360 min after the infusion (p = 0.01) and tended to be higher at the time points 450 min, 48 h, and 72 h (all p = 0.08). Results from this study support the hypothesis that OXY is not directly involved in the mechanism(s) governing blood flow of the CL and has no remarkable effects either on luteal size or P4 and PGFM plasma levels. Further investigation is needed to elucidate the role of OXY in CL blood flow during early and late luteal phases.     

Effects of dietary inclusion of Rhus coriaria on internal milieu of rabbits

The aim of the present work was to investigate the effect of Rhus coriaria L. inclusion to the diet on some biochemical, haematological parameters and the level of antioxidant status of male rabbits. Adult rabbits were divided into five groups: one control (C) and four experimental groups. Experimental animals received sumac per os in feed in various doses (0.50%, 0.75%, 1.00% and 1.50%) for 90 days. Significant increase in PDWc (platelet distribution width) in E3 group when compared with control group was recorded. Sumac administration resulted in decreased cholesterol levels in all experimental groups vs. control group. Significantly lower level of cholesterol was found in E4 group with highest dose of sumac (1.50%). Higher values of total antioxidant status (TAS) and albumins were observed in all experimental groups in comparison with control group. A significant increase in TAS was detected in group E1 and E4. Concentrations of albumins were significantly higher in groups E3 and E4 vs. control group. Sumac administration had no significant effect on bilirubin content. In conclusion, these results show a positive effect of sumac consumption on antioxidant status and cholesterol level in adult male rabbits.     

十二指肠灌注DL-蛋氨酸对肉牛营养物质消化率和血液指标影响的研究

根据基础日粮+待测氨基酸法,由十二指肠瘘管连续灌注DL-Met,通过日增重、氮平衡试验、小肠营养物质消化率指标和血浆游离氨基酸含量确定基础日粮条件下十二指肠氨酸最佳灌注量。试验结果表明:III组日增重最高,比I组提高15.27%(P<0.05)II组和III组沉积氮、沉积氮/可消化氮显著高于I组(P<0.05),其中III组高于II组;III组尿中尿素氮显著低于I组(P<0.05)。III组小肠氮的表观消化率最高,比I组高5.72%(P<0.05)。III组血浆氨氮显著低于I组(P<0.05)。血浆Cys和MetIV组显著高于其它3组(P<0.05),而Ser和Ile则相反。III组血浆Leu、Lys、BCAA和EAA显著低于其它各组(P<0.05)。肉牛十二指肠蛋氨酸最佳灌注量为13g/d。     

热应激对不同品种(系)青年肉牛生产性能、营养物质表观消化率及血液生化指标的影响

本试验旨在研究热应激对不同品种(系)青年肉牛生产性能、营养物质表观消化率及血液生化指标的影响,探究不同品种(系)肉牛的耐热性差异。选取体重[(185.89±14.02)kg]相近、健康的青年西杂牛(西门塔尔牛×宣汉黄牛)、地方黄牛(宣汉黄牛)和犏牛(娟姗牛×麦洼牦牛)各6头为研究对象,试验期间不同品种(系)的青年肉牛饲喂相同饲粮。试验时间为2015年4—9月,预试期7d,正试期180d。结果表明:1)试验牛舍5—8月温湿度指数(THI)高于72,属于热应激期,且显著高于4月(热应激发生前)和9月(热应激发生后)(P0.05);热应激环境导致各试验牛呼吸频率和直肠温度显著升高(P0.05)。试验期间犏牛呼吸频率和直肠温度显著高于西杂牛和地方黄牛(P0.05)。2)热应激期内西杂牛、地方黄牛和犏牛单位体重干物质采食量均有不同程度降低,以6月为最低,均较4月显著降低(P0.05),且降低幅度为犏牛西杂牛地方黄牛。西杂牛、地方黄牛和犏牛的平均日增重(ADG)均在6月最低,分别较4月降低了27.62%(P0.05)、10.81%(P0.05)和46.15%(P0.05);西杂牛的ADG在4—7月显著高于犏牛(P0.05)。西杂牛和犏牛的料重比(F/G)以6月为最高,分别较4月升高了63.01%(P0.05)和89.03%(P0.05);地方黄牛的F/G在9月最高,较4月升高了53.12%(P0.05)。3)热应激期内西杂牛、地方黄牛和犏牛的粗蛋白质(CP)表观消化率均有不同程度降低,且7月均较4月显著降低(P0.05);西杂牛7月的粗脂肪(EE)表观消化率较4月显著降低(P0.05),而热应激对地方黄牛和犏牛的EE表观消化率无显著影响(P0.05);热应激期内犏牛中性洗涤纤维(NDF)(6月)和酸性洗涤纤维(ADF)的表观消化率(5—8月)较热应激发生前显著降低(P0.05);热应激期内西杂牛和犏牛钙(Ca)(西杂牛:6—8月;犏牛:5—8月)和磷(P)的表观消化率(西杂牛:6—7月;犏牛:5—8月)较热应激发生前显著降低(P0.05)。试验期间,不同品种(系)牛的CP表观消化率无显著差异(P0.05),西杂牛和地方黄牛的EE表观消化率显著高于犏牛(P0.05),地方黄牛的ADF表观消化率显著高于西杂牛和犏牛(P0.05),西杂牛和地方黄牛的P表观消化率显著高于犏牛(P0.05)。此外,5—9月地方黄牛的Ca表观消化率显著高于犏牛(P0.05)。4)西杂牛、地方黄牛和犏牛血清中葡萄糖(GLU)浓度均以6月最低,较4月分别降低了16.82%(P0.05)、12.82%(P0.05)和15.90%(P0.05);热应激期间各试验牛血清中非酯化脂肪酸(NEFA)浓度均以7月最低,显著低于4月(P0.05);试验期间西杂牛和地方黄牛血清中尿素氮(UN)浓度随年龄的增大呈先升高后降低变化,而犏牛血清中UN浓度则持续升高;6—9月各试验牛血清肌酐(CRE)浓度均显著高于4、5月(P0.05),且7—9月犏牛血清CRE浓度显著高于地方黄牛(P0.05)。综上所述,热应激导致不同品种(系)青年肉牛生产性能降低、饲粮营养物质消化率降低,地方黄牛的耐热应激能力强于西杂牛和犏牛,犏牛对热应激最敏感。     

REPEATABILITY OF DOPPLER ULTRASONOGRAPHIC MEASUREMENT OF EQUINE DIGITAL BLOOD FLOW

The repeatability and sensitivity of Doppler ultrasonographic measurement of lateral digital arterial and venous blood flow has not been previously determined. Doppler ultrasonography was used to measure blood flow within the forelimb lateral digital vessels in one normal adult Thoroughbred horse on six occasions and in six normal adult Thoroughbred horses on three occasions, each occasion being at least 1 h apart, to determine the within- and between-horse variation. The values obtained from the right and left lateral digital vessels did not differ significantly. The within-horse coefficients of variation (CV) for arterial and venous measurements were all acceptable (< 11%); the between-horse CV were acceptable (< 7%) for all parameters except TaVa (average velocity of first peak of arterial waveform) and TaVb (average velocity of remainder of arterial waveform). The within-horse intraclass correlation coefficients (ICC) demonstrated excellent repeatability (> or = 0.71) for all parameters except venous diameter; the between-horse ICC demonstrated good to excellent repeatability (> or = 0.67) for all parameters except TaVb. Doppler ultrasonography can detect differences of 0.005 and 0.01 ml/ min in digital arterial and venous flow, respectively, using measurements from six horses on three occasions (80% power; P < 0.05). Thus, the technique is sufficiently repeatable and sensitive to be able to detect changes in flow during different physiological or pathological states or following pharmacologic intervention.