首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   2630篇
  免费   305篇
  国内免费   156篇
林业   29篇
农学   57篇
基础科学   6篇
  27篇
综合类   828篇
农作物   33篇
水产渔业   285篇
畜牧兽医   1773篇
园艺   42篇
植物保护   11篇
  2024年   7篇
  2023年   38篇
  2022年   52篇
  2021年   109篇
  2020年   123篇
  2019年   172篇
  2018年   83篇
  2017年   143篇
  2016年   162篇
  2015年   120篇
  2014年   130篇
  2013年   133篇
  2012年   164篇
  2011年   201篇
  2010年   137篇
  2009年   151篇
  2008年   103篇
  2007年   128篇
  2006年   114篇
  2005年   102篇
  2004年   110篇
  2003年   70篇
  2002年   50篇
  2001年   52篇
  2000年   48篇
  1999年   33篇
  1998年   46篇
  1997年   34篇
  1996年   21篇
  1995年   36篇
  1994年   38篇
  1993年   21篇
  1992年   19篇
  1991年   23篇
  1990年   21篇
  1989年   31篇
  1988年   10篇
  1987年   9篇
  1986年   6篇
  1985年   3篇
  1984年   5篇
  1982年   4篇
  1980年   7篇
  1979年   5篇
  1978年   2篇
  1977年   2篇
  1975年   2篇
  1974年   3篇
  1973年   4篇
  1956年   2篇
排序方式: 共有3091条查询结果,搜索用时 31 毫秒
981.
ObjectiveTo evaluate the systemic cardiovascular effects of dose escalating administration of norepinephrine in healthy dogs anesthetized with isoflurane.Study designExperimental study.AnimalsA total of six adult laboratory Beagle dogs, 10.5 (9.2–12.0) kg [median (range)].MethodsEach dog was anesthetized with isoflurane at an end-tidal concentration of 1.7%, mechanically ventilated and administered a continuous rate infusion of rocuronium (0.5 mg kg–1 hour–1). Each dog was administered incremental dose rates of norepinephrine (0.05, 0.125, 0.25, 0.5, 1.0 and 2.0 μg kg–1 minute–1), and each dose was infused for 15 minutes. Cardiovascular variables were recorded before administration and at the end of each infusion period.ResultsNorepinephrine infusion increased mean arterial pressure (MAP), cardiac output (CO) and oxygen delivery in a dose-dependent manner. Systemic vascular resistance did not significantly change during the experiment. Stroke volume increased at the lower dose rates and heart rate increased at the higher dose rates. Oxygen consumption and lactate concentrations did not significantly change during infusions.ConclusionsIn dogs anesthetized with isoflurane, norepinephrine increased MAP by increasing the CO. CO increased with a change in stroke volume at lower dose rates of norepinephrine. At higher dosage, heart rate also contributed to an increase in CO. Norepinephrine did not cause excessive vasoconstriction that interfered with the CO during this study.Clinical relevanceNorepinephrine can be useful for treating hypotension in dogs anesthetized with isoflurane.  相似文献   
982.
试验旨在探讨藏猪低温适应性相关指标,为研究藏猪种质特征提供理论依据,以期更好地开发和利用地方品种资源。在甘肃榆中县某藏猪养殖场随机选择哺乳期间体况良好的20日龄仔猪(分别于冬季环境温度-16 ℃和夏季环境温度30 ℃时选择藏猪各4只),对其体尺指标、呼吸频率、背部脂肪厚度、皮肤厚度、血液生理生化指标进行测量。HE染色观察腹股沟、肩胛、背部的脂肪细胞形态,并进一步采用实时荧光定量PCR技术检测产热相关基因在不同部位脂肪组织间的相对表达情况。结果显示,冬季低温组藏猪的呼吸频率极显著低于夏季高温组藏猪(P<0.01);背部脂肪厚度和颈部、背部、臀部皮肤厚度均极显著高于高温组藏猪(P<0.01);低温组藏猪的血小板数目(PLT)、血小板积压(PCT)、高密度脂蛋白胆固醇(HDL-C)、葡萄糖(GLU)、甘油三酯(TG)含量显著高于高温组藏猪(P<0.05);平均血小板体积(MPV)和血小板分布宽度(PDW)显著低于高温组藏猪(P<0.05)。HE染色结果显示,低温组藏猪腹股沟和肩胛处的脂肪细胞大小不等,存在多腔脂滴形态;同时发现低温组藏猪腹股沟和肩胛部解偶联蛋白3(uncouple protein3,UCP3)、过氧化物酶体增殖物激活受体-γ共激活因子-1α(peroxisome proliferator-activated receptorγcoactivator-1α,PGC-1α)和Ⅱ型脱碘酶(type 2 iodothyronine deiodinase,Dio2)基因mRNA的相对表达量显著高于高温组藏猪(P<0.05)。综上所述,环境温度对藏猪的生理指标和脂肪代谢等方面有较大影响,并且低温刺激白色脂肪褐变是藏猪御寒的重要因素之一。  相似文献   
983.
984.
985.
本研究旨在比较5种DNA提取方法对绵羊血液中布氏杆菌DNA的提取效果及对PCR检测的影响。将不同浓度的疫苗株布氏杆菌加入绵羊全血中,采用3种DNA提取试剂盒和酚/氯仿法以及碘化钠法等5种方法提取模拟的绵羊血液样品中的DNA,评价所获得DNA的浓度、纯度和完整性,并采用布氏杆菌特异性PCR进行检测。同时,对各提取方法所需时间及经济成本进行了比较。结果表明,各方法均能提取获得绵羊全血中布氏杆菌DNA,3种试剂盒和碘化钠法获取布氏杆菌DNA的效果相同,而酚/氯仿法获取布氏杆菌DNA的效率最低或存在PCR抑制剂而不适合用于绵羊血液中布氏杆菌的PCR检测。碘化钠法具有耗时较短、成本低、方便的优点,是从绵羊血液中提取布氏杆菌DNA的良好方法。本研究结果为临床绵羊血液中布氏杆菌DNA提取方法的选择提供了参考。  相似文献   
986.
987.
988.
989.
The present study was carried out to explore the impacts of dietary supplementation of enzyme mixture with sodium butyrate on the growth performance, carcass traits, blood profile and economic benefit in two breeds of weanling rabbits adapted to survive in Egypt (New Zealand White and Rex). One-hundred and twenty weaned male rabbits (New Zealand White and Rex) of 6 weeks of age and 770.5 ± 20 g body weight were allotted randomly into four groups in a factorial arrangement. The obtained results indicated that there were non-significant differences in all growth performance traits, blood profile and economic parameters due to the breed effect. However, there were significant differences in most of carcass traits due to the breed effect except total giblets and New Zealand White breed showed the highest value of these parameters including dressing % (p < .01), forequarter and loin % (p < .001) and hindquarter % (p < .003) compared with Rex breed counterparts. The effect of the treatment and its interaction with the breed significantly (p < .05) improved body weight gain, feed consumption and carcass traits (percentage of dressing, forequarter, hind quarter and lion). However, final body weight and feed conversion ratio were not significantly influenced. Supplementing a diet with treatment significantly decreased blood triglycerides, cholesterol and the ratio between albumin and globulin (A/G ratio), while increased blood total protein and globulin. Although higher feed cost and total costs in treated groups than control ones in each breed, they showed higher total return and net return. Rex non-treated rabbit breed showed the lowest profitability measures compared with other groups. In conclusion, dietary supplementation of multi-enzyme with sodium butyrate is highly recommended in growing rabbits due to their beneficial effects on the growth performance and profitability.  相似文献   
990.
A high amount of blood and not the mere presence of blood in equine semen impacts fertility. The aim of this study was to develop an approach to rescue the fertility of stallions with high hemospermia levels. Semen from 15 stallions was divided into four experimental groups: (a) Control—pure raw semen, (b) WB50—50% (v/v) whole blood added into semen, (c) E1—WB50 extended in a 1:1 (v/v) ratio with milk-based extender and (d) E2—WB50 extended in a 2:1 ratio with milk-based extender. Sperm kinetics, plasma membrane integrity (PMI), lipid peroxidation (PER) and intracellular superoxide (O2) production were immediately evaluated. Four cycles of 20 mares were randomly assigned to the experimental groups. Mares were bred with an insemination dose of 1 billion total sperm and pregnancy was diagnosed 14 days after ovulation. Sperm kinetics could not be evaluated in the WB50 samples. Total motility was lower (p < .05) in E1 than in CT and E2 samples. Progressive motility decreased (p < .05) with an increase in the percentage of blood in the samples. The PMI and PER did not differ between groups (p > .05); however, O2 production was higher (p < .05) in WB50 than in E2 samples, while the values were intermediate (p > .05) for CT and E1 samples. The control (90%) and E2 (90%) groups had superior (p < .05) fertility than the others (WB50—0% and E1—25%). It was concluded that sperm motility and fertility of semen with a large amount of blood can be rescued by dilution with a 2:1 extender:semen ratio using a milk-based extender.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号