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141.
AIM: To explore the regulatory effect of intermedin (IMD) on pulmonary collagen synthesis and accumulation in rats with pulmonary hypertension induced by high pulmonary blood flow.METHODS: Healthy male SD rats (n=20) were randomly divided into control group (n=7), shunt group (n=7) and shunt with IMD group (n=6). The shunting of abdominal aorta and inferior vena cava was produced in rats of shunt group and shunt with IMD group. After 8 weeks, IMD was administered into the rats of shunt with IMD group subcutaneously by mini-osmotic pump for 2 weeks. Mean pulmonary artery pressure (mPAP), relative medial thickness (RMT) of pulmonary arteries, contents of hydroxyproline, collagen type I and III, bone morphogenetic protein-2 (BMP-2), and the mRNA expression of procollagen I and III in lung tissues were measured and compared. RESULTS: Compared with control group, mPAP and RMT of medium and small pulmonary arteries in the rats of shunt group were significantly increased. Meanwhile, the lung hydroxyproline, collagens I and III and BMP-2 contents, and the mRNA expression of lung procollagen I and III were all significantly increased compared with control group. However, IMD significantly decreased mPAP, alleviated the changes of pulmonary vascular micro-structure, decreased the collagen accumulation and pulmonary tissue homogenate BMP-2 contents, and inhibited the mRNA expression of procollagen I and III in the lung tissue of shunting rats.CONCLUSION: IMD plays a protective role in the development of pulmonary hypertension and pulmonary vascular structural remodeling induced by high blood flow by inhibiting pulmonary collagen synthesis and accumulation, possibly in association with the BMP-2 pathway. 相似文献
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144.
采用四因素三水平正交试验,对藏羊血制备食用蛋白的工艺进行了分析。经分析食用蛋白粉水解最佳条件为:酶解用酶量4.5g,酶解温度为46℃,酶解时间为7.5h,酶解的pH值为10.5,活性炭作用于水解液时由室温升至80℃所需最佳时间为6~8min。 相似文献
145.
Ki-Soo Park Yong-Soon Lee Kyung-Sun Kang 《Journal of veterinary science (Suw?n-si, Korea)》2006,7(4):343-348
Mesenchymal stem cells (MSCs) have the capabilities for self-renewal and differentiation into cells with the phenotypes of bone, cartilage, neurons and fat cells. These features of MSCs have attracted the attention of investigators for using MSCs for cell-based therapies to treat several human diseases. Because bone marrow-derived cells, which are a main source of MSCs, are not always acceptable due to a significant drop in their cell number and proliferative/differentiation capacity with age, human umbilical cord blood (UCB) cells are good substitutes for BMCs due to the immaturity of newborn cells. Although the isolation of hematopoietic stem cells from UCB has been well established, the isolation and characterization of MSCs from UCB still need to be established and evaluated. In this study, we isolated and characterized MSCs. UCB-derived mononuclear cells, which gave rise to adherent cells, exhibited either an osteoclast or a mesenchymal-like phenotype. The attached cells with mesenchymal phenotypes displayed fibroblast-like morphologies, and they expressed mesenchym-related antigens (SH2 and vimentin) and periodic acid Schiff activity. Also, UCB-derived MSCs were able to transdifferentiate into bone and 2 types of neuronal cells, in vitro. Therefore, it is suggested that the MSCs from UCB might be a good alternative to bone marrow cells for transplantation or cell therapy. 相似文献
146.
Summary Data from two Swedish and one Finnish open-top chamber experiments were combined to investigate effects of ozone exposure
on potato (Solanum tuberosum L.) tuber content of starch, sucrose, fructose, glucose, malic, citric and ascorbic acids. The
glucose, fructose and malic acid concentrations showed strong negative correlations with ozone exposure, while citric acid,
consistently increased with ozone exposure. No ozone effects could be demonstrated on starch, sucrose or ascorbic acid concentrations.
It is discussed to what extent the changes found in potato tuber composition can be explained in terms of ozone effects on
tuber maturity. Ozone exposure was expressed as the accumulated exposure over a cut-off concentration of 40 nmol mol−1 (AOT40) and as the accumulated uptake of ozone over an ozone uptake rate threshold of 7 nmol m−2 s−1 (CUO 7). The difference in ability of the exposure indices to explain observed effects was small. 相似文献
147.
Garofalo NA Teixeira Neto FJ Alvaides RK de Oliveira FA Pignaton W Pinheiro RT 《Veterinary anaesthesia and analgesia》2012,39(4):324-334
ObjectiveTo evaluate the agreement between invasive blood pressure (IBP) and Doppler ultrasound blood pressure (DUBP) using three cuff positions and oscillometric blood pressure (OBP) in anesthetized dogs.Study designProspective study.AnimalsNine adult dogs weighing 14.5–29.5 kg.MethodsThe cuff was placed above and below the tarsus, and above the carpus with the DUBP and above the carpus with the OBP monitor. Based on IBP recorded via a dorsal pedal artery catheter, conditions of low, normal, and high systolic arterial pressures [SAP (mmHg) <90, between 90 and 140, and >140, respectively] were induced by changes in isoflurane concentrations and/or dopamine administration. Mean biases ± 2 SD (limits of agreement) were determined.ResultsAt high blood pressures, regardless of cuff position, SAP determinations with the DUBP underestimated invasive SAP values by more than 20 mmHg in most instances. With the DUBP, cuff placement above the tarsus yielded better agreement with invasive SAP during low blood pressures (0.2 ± 16 mmHg). The OBP underestimated SAP during high blood pressures (?42 ± 42 mmHg) and yielded better agreement with IBP for mean (MAP) and diastolic (DAP) arterial pressure measurements [overall bias: 2 ± 15 mmHg (MAP) and 0.2 ± 16 mmHg (DAP)].ConclusionsAgreement of SAP determinations with the DUBP is poor at SAP > 140 mmHg, regardless of cuff placement. Measurement error of the DUBP with the cuff placed above the tarsus is clinically acceptable during low blood pressures. Agreement of MAP and DAP measurements with this OBP monitor compared with IBP was clinically acceptable over a wide pressure range.Clinical relevanceWith the DUBP device, placing the cuff above the tarsus allows reasonable agreement with IBP obtained via dorsal pedal artery catheterization. Only MAP and DAP provide reasonable estimates of direct blood pressure with the OBP monitor evaluated. 相似文献
148.
饲粮中添加铬——烟酸复合物对肉鸭生产性能、胴体成分及血液生化指标的影响 总被引:13,自引:3,他引:13
选择1日龄樱桃谷母鸭240只 ,随机分为3组 ,每组5个重复 ,每个重复16只 ,饲养49天。在各组鸭的饲粮中分别添加0、100、200μm/kg铬量的铬—烟酸复合物 ,研究铬—烟酸复合物对肉鸭生产性能、胴体成分及血液生化指标的影响。试验结果显示 :(1)饲粮中添加铬 -烟酸复合物显著(P<0.01)提高肉鸭体重和日增重 ,显著(P<0.01)降低料肉比 ,对日采食量无影响(P>0.05) ;(2)添加铬 -烟酸复合物显著(P<0.05)提高肉鸭瘦肉率和胴体粗蛋白质含量 ,显著(P<0.05)降低肉鸭皮脂率和胴体粗脂肪含量 ;(3)添加铬 -烟酸复合物显著(P<0.01)降低肉鸭血清总脂、葡萄糖、总胆固醇、三酰甘油和尿素氮含量 ,显著(P<0.01)提高血清总蛋白含量。试验结果表明 :饲粮中添加200μm/kg铬量的铬 -烟酸复合物不仅可促进肉鸭生长、降低料肉比 ,而且可提高屠宰性能 ,改善胴体品质 相似文献
149.
陈学文 《青海畜牧兽医杂志》1998,28(5):7-9
1994~1996年在海南州赛什塘牧场对1/2和1/4野血牦犊牛的生长发育进行了观测。结果:1/2和1/4野血牦犊牛的初生重分别比家牦犊牛提高7.65%和8.86%(P<0.01),1/2野血牦犊牛在初生时的体高、体长和胸围比家牦犊牛分别提高3.21%,5.57%,5.51%;6月龄时分别提高9.56%,11.46%,3.59%;18月龄时分别提高9.18%,17.55%,7.64%(P<0.01)。 相似文献
150.