复杂荷载作用下单轴对称开口薄壁梁柱的弯扭屈曲

采用Rayleigh-Ritz法求解两端等弯矩、横向集中荷载和均布荷载作用下单轴对称开口薄壁截面梁柱弯扭屈曲临界荷载。分析中考虑了3种常见边界条件以及屈曲前变形的影响。将本文弯扭屈曲临界荷载理论值与试验值比较，吻合较好。     

再生稻高产栽培综合农艺措施研究

用二次回归正交旋转组合设计，研究了再生稻综合农艺措施对产量的影响，建立了相应数学模型，得出了最佳逐艺措施组合方案。即在重庆地区，再生稻用促芽氮肥１４５．１６－１５７．５２ｋｇ／ｈａ，于头季稻齐穗后２２－２４天施，头季稻齐穗后１５天配施磷钾９９．９５－１０８．１１ｋｇ／ｈａ，留桩高度２４－２８ｃｍ，发苗肥４８．３４－５７．５８ｋｇ／ｈａ，再生和妥产量可达１８９０ｋｇ／ｈａ以上。     

SJ—1型数字粮食水分计的研制

Sj—1型数字粮食水分计采用平行板电容器作为传感器,较通用的圆柱形电容传感器性能稳定。测量电路及显示电路均采用数字电路。非电量经A/D转换后,通过EPROM查表的方法,实现了数据的线性化,测量精度高。     

Evaluation of Root Sink Ability of Sweet Potato (Ipomoea batatas Lam.) Cultivars on the Basis of Enzymatic Activity in the Starch Synthesis Pathway

Activities of five enzymes, sucrose synthase (SUS), uridine 5'-diphosphate glucose pyrophosphorylase (UDPGPPase), fructose-1,6 bisphosphatase (FBPase), adenosine 5'diphosphate glucose pyrophosphorylase (ADPGPPase) and starch synthase (STS), in the metabolic pathway of starch synthesis, were compared between two sweet potato ( Ipomoea batatas Lam.) cultivars, Koganesengan (C.V.KOG, a recently released high yield cultivar) and Tsurunasigenji (C.V.TSU, an old, local cultivar with poor yield). The measurements were carried out using the root samples (tuberous, thick and fibrous roots) harvested at the fast tubering stage. Of the five enzymes, SUS, ADPGPPase and STS showed high activities in the tuberous root, particularly in that of C.V.KOG, and a similar trend was observed for activities of these enzymes on a protein basis. The increased activity of the three enzymes is considered to be one of the characteristics in a high yield cultivar, allowing the root to function effectively as a starch synthesis and storage organ.     

猴头菌营养生长最佳条件研究

对4个猴头菌菌株的研究表明,猴头菌营养生长阶段最佳碳源为淀粉,最佳氮源为麦麸及蛋白胨,最适碳氮比为20∶1,最适pH为4.0～5.0,最适温度为25～29℃。     

Protein Phosphorylation and Protein Patterns in Developing Endosperms of Barley

The SDS-soluble protein-fractions of barley endosperms (2 and 3 weeks after anthesis) were compared using 1-D SDS-PAGE and 2-D IEF/SDS-PAGE. Similar procedures have been followed after in vivo phosphorylation of isolated endosperm with ³²PO₄.Protein patterns were similar when 1-D SDS-PAGE was used. On the other hand, 2-D IEF/SDS-PAGE revealed several major differences, the most important being the appearance of a major protein of Mr about 40,000 in the 3-weeks old endosperm. By contrast, the patterns of phosphorylated proteins, with both methods, were relatively simple. Only two phosphorylated bands showed up in the 3-weeks old endosperm: a highly labelled band of Mr about 65,000, and a band of about 75,000. In the case of 2-weeks old endosperm, where the phosphorylation was more active, these and several others were also labelled, but the Mr 75,000 band was labelled more intensely.     

罗布麻韧皮非纤维素生物降解的工艺基础

在综合国内外相关研究文献资料的基础上,介绍了开发罗布麻纤维的研究现状、麻类脱胶菌种及其工艺研究、麻类脱胶机理研究的基础工作。同时,指出了罗布麻韧皮中非纤维素生物降解研究的难点在于规范收获方法。     

犬腺病毒纤突蛋白基因功能区的比较分析

根据GenBank中已发表的CAV纤突基因序列，设计合成4对8条引物，用已建立的PCR方法，对犬2型腺病毒沈阳分离株第5代强毒经蚀斑克隆驯化的第60代毒弱毒（SY-V60）和犬1型腺病毒长春犬株（CCC-V6）的纤突基因进行了扩增，PCR产物经纯化后进行基因序列测定，测定结果经拼接后得到一个由1632和1629个核苷酸组成的纤突蛋白全基因序列，分别编码543和542个氨基酸。犬2型腺病毒（SY-V60）与犬1型腺病毒（CCC-V6）的纤突基因的同源性达到80.48%。犬2型腺病毒（SY-V60）与犬1型腺病毒（CCC-V6）纤突基因的同源性达到80.48%；根据犬的腺病毒与人2型腺病毒纤突蛋白的序列同源性比较结果，推测了犬腺病毒纤突蛋白的尾（tail)、轴（shaft)，结（knob)三个功能区的氨基酸序列，2个型之间的尾区同源性为76.9%；轴区同源性为78.59%；其结区同源性为83.24%，而同型毒株之间结和尾区同源性较高，轴区同源性较低。     

基于Web的集成化产品数据管理方法的研究

对基于浏览器／服务器（Browser/Server）结构的信息管理方式进行了研究，并提出了基于Web的产品数据管理（PDM）方法，通过引入Web机制，实现集成与共享，以支持先进制造模式。     

伪狂犬病病毒Bartha株TK-/LacZ+突变株的构建及其生物学特性研究

本研究将伪狂犬病病毒Bartha株基因组与含有LacZ标志基因的TK基因转移质料pUEKPZ共转染猪肾传代细胞PK－5，细胞出现病变后，反复冻融3次收毒，按1：5稀释接种于IBRS－2细胞。在X－gal存在下挑取蓝斑，蓝斑筛选3次，再进行空斑试验，同时用PCR扩增LacZ基因，经3次空斑纯化，随机挑取的空斑均能扩增出LacZ基因，证实所获得的重组病毒为伪狂犬病病毒Bartha株TK^-/LacZ^ 突变株。TCID50试验表明，TK失活对Bartha株在细胞上增殖无影响；Balb/C小鼠试验表明，该突变对Balb/C小鼠的安全性明显高于Bartha亲本毒株。