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151.
青贮玉米促生菌的鉴定及生物学特性的研究 总被引:2,自引:0,他引:2
根据布坎南.R.E等著《伯杰细菌鉴定手册》(第八版),对4株青贮玉米促进菌进行鉴定。结果表明,4株促生菌分属于短芽胞杆菌(Bacillus brevis),蜡质芽孢杆菌(Bacillus cereus),放射形土壤杆菌(Agrobacteria radiobacter)和假单胞杆菌(Pseudomonas sp)。此餐,还对4株促生菌的生物学特性进行了比较观察。 相似文献
152.
Xanthomonas axonopodis pv. glycines ( Xag ) causes bacterial pustule disease which can significantly reduce the production of soybean. A collection of 26 isolates of Xag from different soybean-production areas of Thailand was shown to differ with regard to aggressiveness on soybean. They also differed in their ability to induce a hypersensitive response (HR) on four cultivars of tobacco and on other plant species including pepper, tomato, cucumber, pea and sesame. Tomato was most sensitive to HR induction by Xag . Isolate KU-P-34017 caused an HR on all the plant species tested. The minimal concentration of KU-P-34017 needed to induce HR on tobacco was approximately 5 × 108 CFU mL−1 . A bacterium–plant interaction period of at least 2·5 h was necessary for HR, and different temperatures, relative humidity and light periods did not affect HR development. Inhibitors of eukaryotic metabolism, including cobalt chloride, lanthanum chloride and sodium orthovanadate (completely), and cycloheximide (partially) blocked the HR on tobacco, indicating the association of an active plant response. In contrast, the HR on tomato was inhibited only by cobalt chloride. 相似文献
153.
Teruo NONOMURA Yoshinori MATSUDA Motoaki TSUDA Kazuto URANAKA Hideyoshi TOYODA 《Journal of General Plant Pathology》2001,67(3):224-227
Commercially available tomato cultivars were hydroponically cultured for inoculation, with Ralstonia solanacearum (K-101), which causes bacterial wilt, by pouring an inoculum suspension into the nutrient solution. Cultivar susceptibility
to the bacteria was evaluated, based on the highest percentage of wilting. Because the length of time for wilt appearance
varied among cultivars, some cultivars appeared to be suppressive to the translocation and/or multiplication of the invading
pathogen. Thus, this hydroponic inoculation system is effective for examining levels of susceptibility in tomato cultivars
to bacterial wilt.
Received 13 December 2000/ Accepted in revised form 27 March 2001 相似文献
154.
Seiji TSUGE Ayako FURUTANI Rie FUKUNAKA Yasuyuki KUBO Osamu HORINO 《Journal of General Plant Pathology》2001,67(1):51-57
Xanthomonas oryzae pv. oryzae (X. o. pv. oryzae) T7174 is virulent on rice cultivar IR24 and avirulent on IR-BB2. From recent reports, some virulence and avirulence factors
of plant pathogenic bacteria are transferred to plant cells through the hrp-dependent type III secretion system. In this study, we investigated the involvement of hrp genes in the compatible and the incompatible interactions between rice and X. o. pv. oryzae after co-inoculation with hrpXo mutants derived from T7174 and virulent strains. Growth of the mutants, named 74ΔHrpXo and 76ΔHrpXo, was repressed in IR24 when the mutants were applied alone. However, growth of the mutants was complemented by co-inoculation
with virulent strains. Growth of bioluminescent hrpXo mutant 76ΔHrpXo in IR24 and its growth in IR-BB2 after co-inoculation with T7133, which is virulent on both cultivars, was equally complemented,
as detected by bioluminescence from the mutant. On the other hand, only partial complementation of growth of T7174L76, which
is a bioluminescent and pathogenic derivative of T7174, by T7133 was observed in IR-BB2. Thus, growth of the hrpXo mutant of X. o. pv. oryzae was complemented by virulent strains in both susceptible and resistant rice leaves with the parental strain.
Received 21 July 2000/ Accepted in revised form 26 October 2000 相似文献
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应用鲎试剂动态浊度法,将硫酸链霉素制备成4、2、1、0.5mg/mL溶液,定量添加标准内毒素进行干扰预试验,计算回收率,确定不干扰浓度为0.5—2mg/mL。从中选出1mg/mL为最佳浓度,然后进行3个批次硫酸链霉素正式干扰试验。在供试品中定量添加标准内毒素,其回收率分别为125.8%、126.1%、127.3%,均在50%-200%之间,说明此浓度的供试品对鲎试剂反应无干扰作用。因此,内毒素含量在0.015—1EU/mL范围内,将硫酸链霉素制备成浓度为1.0mg/mL的溶液,可用于细菌内毒素的定量检测。 相似文献