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81.
酶标抗原Dot-ELISA检测鸭肝炎病毒抗体 总被引:4,自引:0,他引:4
酶标抗原Dot-ELISA检测鸭肝炎病毒抗体@杨奎@陈溥言... 相似文献
82.
R Sanchis 《Comparative immunology, microbiology and infectious diseases》1980,3(4):517-523
The bacteriological examination of 112 strains of Salmonella abortus ovis shows some particular properties of this serotype, and the existence of two different types according to their geographical source. 相似文献
83.
E Brummerstedt 《Acta veterinaria Scandinavica》1976,17(4):395-402
Counterimmunoelectrophoresis as a test method for making the diagnosis of plasmacytosis in mink demands the specific virus antigen. The method for preparation of the antigen according to Cho & Ingram (1972 a, b) with minor modifications is described in details, and results obtained at 62 antigen preparations are presented. In addition an ultrafiltration method is outlined which may be useful as a replacement for ultracentrifugation procedures used in the technique described by Cho & Ingram (1974). 相似文献
84.
G Holstad 《Acta veterinaria Scandinavica》1986,27(4):575-583
The bacterial agglutination test (BAT) and the hemolysis inhibition test (HIT) were employed and evaluated.The threshold value between positive and negative antibody titres in BAT and HIT was determined on the basis of titration results for sera from 25 adult goats infected with Corynebacterium pseudotuber-culosis (positive), and sera from 25 adult goats from a herd in which caseous lymphadenitis did not occur (negative).Antibody titres were expressed as logio to the reciprocal value of the highest positive serum dilution in both tests. Positive titre (T) in BAT was stipulated as T ≥ 2.1 and in HIT as T > 0.6. The sensitivity and specificity was 0.96 in both tests in the material used. Twenty-three of the 25 goats infected with G. pseudotuberculosis were positive in both BAT and HIT.The reproducibility in both tests was described by the use of correlation coefficient and coefficient of variation. The values were estimated using duplicate determination of titres of 155 serum samples. Correlation coefficients were 0.87 for BAT and 0.95 for HIT. Coef-ficients of variation were 26.4 % for BAT and 14.1 % for HIT. The coefficient of variation was related to titres. It was highest for BAT at low titres.It was concluded that both tests can be used for seroepidemio-logical investigations of C. pseudotuberculosis infection in goats. 相似文献
85.
86.
接种泰氏锥虫及其抗原使家兔和小鼠产生抗伊氏锥虫的交叉免疫力 总被引:2,自引:0,他引:2
用培养的泰氏锥虫制备的死、活两种虫体抗原,接种于家兔和小鼠,一定时间后用伊氏锥虫强毒株攻击,观察其血清抗体的变化及交叉免疫保护能力。试验表明,泰氏锥虫虫体抗原可刺激机体产生较高的体液抗体;免疫动物有一定的抵御伊氏锥虫攻击的能力,与对照组相比,免疫动物血液中虫体出现的时间较迟,最初的数量少,临床症状轻。死虫抗原组的交叉免疫保护能力较活虫抗原组强。 相似文献
87.
片形吸虫分泌排泄抗原和虫体抗原的分析 总被引:1,自引:0,他引:1
用牛源肝片吸虫(南京)、牛源大片吸虫(广西)和羊源肝片吸虫(实验感染)制备可溶性虫体抗原(BA)和和分泌排泄抗原(ES),以SDS-PAGE电泳分析比较,结果显示,3株虫体可溶性虫体抗原至少有20条以上的主要蛋白条带,分子量集中于10-90KD之间,它们之间无显著差异,而3株分泌排泄抗原蛋白成分较简单,明显可分的条带不超过5条,分子量集中于10-30KD之间,且均拥有26-28KD的蛋白成分,提示该蛋白应为片形吸虫ES抗原的主要免疫成分。 相似文献
88.
Finn Saxegaard 《Acta veterinaria Scandinavica》1981,22(2):153
One hundred and two Mycobacterium avium and M. avium-like strains isolated from domestic and wild animals were submitted to serological typing by means of the agglutination method developed by Schaefer. Of the 82 porcine strains M. intracellulare serotype 8 dominated, followed in order of frequency by serotypes 1, 4, 2, 10 and 6. Of the 20 strains originating from other animals 7 were typed as either serotype 2, 3, 8 or 10. The 3 strains isolated from wild birds were all serotype 2. As a considerable number of the wild animal strains were autoagglutinable, the suitability of the agglutination test for typing such strains is discussed. 相似文献
89.
Gall D Nielsen K Bermudez RM Muñoz del Real MC Halbert G Groulx R Moreno F Chow EY Checkley SL 《Research in veterinary science》2006,81(2):215-217
An indirect enzyme-linked immunosorbent assay (IELISA) was developed for the detection of equine serum antibodies to lipopolysaccharide of Salmonella enterica subsp. enterica serovar Abortusequi (LPS), a causative organism of Equine Paratyphoid. The data presented demonstrates that horses immunized with S. abortusequi LPS developed antibodies detectable by the IELISA. By comparison, the tube agglutination test (TAT) did not detect antibody to S. abortusequi LPS as consistently as the IELISA. The data suggests that the IELISA may be a more suitable test for the detection of serum antibodies to S. abortusequi than the TAT. 相似文献
90.