Effects of Mixed Caulis Lonicerae japonicae Extracts on Fracture Healing and Anti-inflammation

This experiment was aimed to study the effects of mixed Caulis Lonicerae japonicae extracts on fracture healing and inflammation. Rabbit right radius fracture model was induced by dental drill in order to study the medicine's effect on fracture healing. Moreover,the contents of Ca,P and alkaline phosphatase in serum were measured to explore the influences of mixed Caulis Lonicerae japonicae extracts on serum biochemical indexes of fractured rabbits.RAW264.7 cell was induced by LPS in order to research the effect of medicine on inflammation. The concentrations of TNF-α,IL-1β,IL-6 and NO were measured by ELISA. The results showed that the rabbit fracture model was successfully prepared by dental drill, and the extracts of Caulis Lonicerae japonicae could increase the levels of Ca and alkaline phosphatase and reduce the P content in the serum of fractured rabbits. The TNF-α,IL-1β and NO levels in RAW264.7 cell induced by LPS were significantly inhibited by the extracts with concentrations ranging from 50 to 200 μg/mL (P<0.05). However, the extracts had no significant effect on the level of IL-6 (P>0.05). These results demonstrated that the extracts of mixed Caulis Lonicerae japonicae could promote the fracture healing and possessed good resistance inflammation effect.     

鸡蛋提取物角蛋白及鸡蛋黄对兔伤口愈合的临床研究

为了探讨鸡蛋提取物角蛋白及鸡蛋黄对伤口愈合影响的临床研究,取健康家兔12只,随机分为A、B两组,每组6只,在每只家兔脊背两侧设试验伤口和对照伤口各1个,即同体不同部位对照试验;A组试验处伤口涂抹鸡蛋提取物角蛋白;B组试验处伤口涂抹生鸡蛋黄;A、B两组对照处伤口均未作任何处理。结果表明:A组和B组涂抹鸡蛋提取物角蛋白和生鸡蛋黄的伤口愈合时间比未做任何处理的伤口愈合快3～4 d,无论是愈合速度还是愈合效果均非常明显且伤口感染率减少,伤口愈合的疤痕减淡,外部更加美观自然,且能达到祛疤美容效果。     

Reactions of goldfish (Carassius auratus) to three suture patterns following full thickness skin incisions

Three different suture patterns (simple interrupted, interrupted horizontal mattress, subcuticular) were placed in a full thickness incision (skin and body wall) of 18 goldfish (Carassius auratus). After 14 days all fish were euthanized using benzocaine solution. The tissue reactions were evaluated by gross visual inspection and histopathological examination. The superficial inflammatory reactions were graded on a scale from 0 (no inflammation) to 3 (severe inflammation). The inflammatory response in histological examination was graded on a scale from 0 (no inflammatory response or normal skin tissue) to 5 (severe inflammatory response and necrosis). The interrupted horizontal mattress induced a moderately severe to severe inflammatory response and necrosis (grades 4–5) but the subcuticular suture induced a very mild to mild inflammatory response (grades 0–1). The simple interrupted suture induced a moderate to moderately severe inflammatory response (grades 2–4). In conclusion, results showed that a subcuticular suture is the most appropriate to use in the closure of a full thickness body wall incision.     

蚕蛹壳聚糖复合水凝胶的抗菌性能及对创伤的愈合作用

以蚕蛹壳聚糖、蜂蜜和明胶等为基质制备医用复合水凝胶。对以不同配比制备的蚕蛹壳聚糖复合水凝胶、单纯蚕蛹壳聚糖水凝胶、蜂蜜水凝胶的抗菌性能进行比较试验,并以大耳白兔为受试动物,采用切片法检测蚕蛹壳聚糖复合水凝胶对烫伤伤口的愈合作用。结果表明:用质量分数为0.25%的蚕蛹壳聚糖和质量分数为20%的蜂蜜制成的复合水凝胶的杀菌作用明显高于市售烧伤膏和单纯蚕蛹壳聚糖或蜂蜜水凝胶,对金黄色葡萄球菌、大肠杆菌24 h内的抑菌率均达到100%,且持久性好;大耳白兔烫伤伤口经0.25%蚕蛹壳聚糖+20%蜂蜜复合水凝胶处理后12 d可基本愈合,切片观察愈合的伤口表皮无溃疡,局部毛囊增生,且有新生表皮细胞生成。研究结果显示,蚕蛹壳聚糖复合水凝胶具有广谱抗菌性能,可用作创伤敷料。     

Efficacy of nano-hydroxyapatite prepared by an aqueous solution combustion technique in healing bone defects of goat

The present study was undertaken to evaluate porous hydroxyapatite (HAp), the powder of which was prepared by a novel aqueous solution combustion technique, as a bone substitute in healing bone defects in vivo, as assessed by radiologic and histopathologic methods, oxytetracycline labeling, and angiogenic features in Bengal goat. Bone defects were created in the diaphysis of the radius and either not filled (group I) or filled with a HAp strut (group II). The radiologic study in group II showed the presence of unabsorbed implants which acted as a scaffold for new bone growth across the defect, and the quality of healing of the bone defect was almost indistinguishable from the control group, in which the defect was more or less similar, although the newly formed bony tissue was more organized when HAp was used. Histologic methods showed complete normal ossification with development of Haversian canals and well-defined osteoblasts at the periphery in group II, whereas the control group had moderate fibro-collagenization and an adequate amount of marrow material, fat cells, and blood vessels. An oxytetracycline labeling study showed moderate activity of new bone formation with crossing-over of new bone trabeculae along with the presence of resorption cavities in group II, whereas in the control group, the process of new bone formation was active from both ends and the defect site appeared as a homogenous non-fluoroscent area. Angiograms of the animals in the control group showed uniform angiogenesis in the defect site with establishment of trans-transplant angiogenesis, whereas in group II there was complete trans-transplant shunting of blood vessel communication. Porous HAp ceramic prepared by an aqueous combustion technique promoted bone formation over the defect, confirming their biologic osteoconductive property.     

The Healing Potential of Plantago lanceolata Ointment on Collagenase-Induced Tendinitis in Burros (Equus asinus)

This study was carried out to evaluate the healing potential of water-soluble extract of Plantago lanceolata as a topical ointment on experimental tendinitis in burros. Tendinitis was induced by injection of 2,500 U of bacterial collagenase in the superficial digital flexor tendon of both forelimbs of six male burros. Tendinitis was confirmed ultrasonographically. 48 hours after collagenase injection, two grams of 10% P lanceolata ointment was applied on one limb (treatment group) and the same amount of eucerin (the vehicle of the ointment) on the other limb (control group), every other day for 6 weeks. Ultrasonographic examination of the tendon was performed weekly during the study and mean cross-sectional area of the superficial digital flexor tendon was measured, which was found to increase significantly after injection of collagenase in both groups. From the third week, it decreased significantly in the treatment group in comparison with the control group. At the end of the study, the animals were euthanized for histopathological study of the tendons. In control group, degenerative changes in tendon could be seen, whereas significant degree of healing was observed in the treatment group. It can thus be concluded that the P lanceolata ointment was effective in promoting the healing process of the tendon.     

Evaluation of bone healing in canine tibial defects filled with cortical autograft,commercial-DBM,calf fetal DBM,omentum and omentum-calf fetal DBM

The present study was conducted to compare the effects of xenogenic bovine fetal demineralized bone matrix (DBM), commercial DBM, omentum, omentum-calf fetal DBM, cortical autograft and xenogenic cartilage powder on the healing of tibial defects in a dog model to determine the best material for bone healing. Seven male adult mongrel dogs, weighing 26.2 ± 2.5 kg, were used in this study. Seven holes with a diameter of 4-mm were created and then filled with several biomaterials. Radiographs were taken postoperatively on day 1 and weeks 2, 4, 6, 8. The operated tibias were removed on the 56th postoperative day and histopathologically evaluated. On postoperative days 14, 42 and 56, the lesions of the control group were significantly inferior to those in the other group (p < 0.05). On the 28th postoperative day, the autograft group was significantly superior to the control and omentum groups (p < 0.05). Moreover, calf fetal DBM was significantly superior to the control group. There was no significant difference between the histopathological sections of all groups. Overall, the omentum and omentum-DBM groups were superior to the control group, but inferior to the autograft, commercial-DBM, calf fetal DBM and calf fetal cartilage groups.     

纤维蛋白胶复合物促进犬骨折愈合的临床学观察

为了探索纤维蛋白胶(FG)联合人骨形态发生蛋白(rhBMP-2)、碱性成纤维细胞生长因子(bFGF)和妥布霉素的复合物对犬骨折愈合的影响,建立了犬胫骨骨折模型,将FG复合物应用于实验组和空白对照组的胫骨骨折处,在术后2、4、8、12、16周,测定骨折处周围软组织充血水肿消除时间、利用X射线影像学评价骨折愈合程度、测定骨折愈合部位的骨密度(BMD)及骨密度比率、骨痂体积,测定胫骨生物力学强度并进行统计和分析。实验组和对照组比较:术部红、肿、热消失时间显著缩短,术后各时间点的X射线影像学评分更高,术后各时间点骨痂更大,术后16周术部骨骼能承受的最大载荷和最大位移显著增大。结果表明FG联合rhBMP-2、bFGF与妥布霉素应用于骨折部位,可促进骨折愈合过程中软组织的生成,缩短骨折愈合的时间,提高骨生物力学强度。