Motility Parameters of Rainbow Trout (<Emphasis Type="Italic">Oncorhynchus mykiss</Emphasis>) Spermatozoa in Relation to Sequential Collection of Milt,Time of <Emphasis Type="Italic">Post-mortem</Emphasis> Storage and Anesthesia

The present study investigated the effects of sequential collection of milt, time of post-mortem storage and anesthesia on rainbow trout (Oncorhynchus mykiss) sperm motility parameters (using computer-assisted sperm analysis – CASA) as well as seminal plasma osmolality and sperm concentration. The post-mortem storage and time of anesthesia altered motility characteristics of rainbow trout sperm to different extents. The moderate impact of time of anesthesia was manifested in a shortened duration of sperm motility after 10 min exposure of fish to anesthetic. The prolonged post-mortem storage (≥40–60 min), in addition to lowering sperm motility duration, also significantly influenced sperm motility parameters, such as sperm velocities, percentage of motile sperm and sperm trajectory parameters. These results clearly demonstrate that when milt from sacrificed fish is used for sperm motility studies, the time of post-mortem storage significantly alters sperm motility characteristics. Since sperm motility rate and swimming velocity could predict fertilizing ability, detrimental effects of prolonged post-mortem storage may lead to reduced fertilization success. Sperm concentration and seminal plasma osmolality were lower in the first fractions and increased with successive collections of milt. It suggests the presence of urine contamination of the first milt fractions which were collected by stripping. Therefore, testing of sperm concentration and/or seminal plasma osmolality should be mandatory while handling stored milt.     

山羊卵泡卵母细胞的采集及体外成熟效果研究

为了获取高质量的卵泡卵母细胞，提高山羊体外胚胎生产的效率，采用切剖法与抽吸法采集卵泡卵母细胞，并对获得的可用卵母细胞进行了体外成熟培养。结果表明，切剖法能够获得较多的可用卵母细胞，可以明显增加用于体外成熟的卵母细胞数；卵母细胞的体外成熟率与卵泡直径密切相关，大卵泡与中卵泡的卵母细胞成熟率明显高于(P<0.05)小卵泡；山羊卵泡卵母细胞的体外成熟培养液中FCS的添加比例以10% ~ 15%为益。在一定范围内，提高FCS的浓度，并不能提高卵母细胞的成熟率。     

中文图书藏用情况量化分析

运用自动化管理系统,检索某一时期内该馆中文流通图书入藏利用情况,量化分析了读者需求与藏书发展的关系,探索了藏用协调发展的规律,对藏书发展工作具有一定的借鉴意义。     

基于表型多样性构建向日葵核心种质

为鉴定评价我国向日葵（Helianthus annuus L.）种质资源在南方地区的表现,筛选构建向日葵核心种质,以422份向日葵种质为材料,在进行2年鉴定观察的基础上,采用描述性统计、相关性分析、主成分分析等方法对11个表型农艺性状进行分析评价。结果表明,原始群体的这些农艺性状具有较大的变异幅度,其变异系数（CV）为3.60%~83.32%,平均变异系数为20.93%,其中分枝株率（0%~62.5%）、单株粒重（9.70~232.35 g）、百粒重（4.60~14.92 g）、叶片数（14.40~48.38个）和株高（103.75~260.00 cm）变异幅度较大;性状间表现出显著的相关性,主成分分析表明,影响性状的4个主要成分解释了总方差的71.72%。采用QGAstation 2.0软件构建了72组核心种质候选群体,并根据均值差异百分数（MD）、方差差异百分率（VD）,极差符合率（CR）和变异系数百分率（VR）,获得组获得1组包含84份材料的最佳核心种质群体。聚类分析将84份核心资源分为5大类,与原群体相比,所选核心种质均值无显著差异,方差显著提高,能最大限度代表原始油葵种质资源保存和利用。     

啤酒花品种

本研究于1980～1988年搜集了中国陕西、新疆的野生啤酒花资源和国内外栽培啤酒花品种(品系)共66份,经整理实际集搜陕西、新疆野生啤酒花的雄株、雌株和47个品种(品系)。通过10余年保存,对种植保存的种植方法和组织培养保存法均提出了一些意见。     

眼镜蛇取毒饲养生产试验

常山县龙尧乡进行眼镜蛇取毒饲养试验表明：取毒时间，每１０ｄ取毒１次，可正常饲养，同时也提高了蛇毒产量。每１ｋｇ眼镜蛇，可得蛇毒液７．０８２４ｇ／ａ，蛇毒干品２．０８３２ｇ／ａ。     

云南裂褶菌宿主多样性及生境调查

 首次对滇中、滇南、滇西以及滇西南地区不同宿主、生境的野生裂褶菌Schizophyllum commune资源进行采集,并阐述了裂褶菌宿主特性以及生长地的生境条件。分离到不同宿主、生境的裂褶菌菌株407个,宿主植物共75种,分属于30科,其中单子叶植物4种,双子叶植物64种,裸子植物7种。菌株生态分布的海拔范围为372～3321m。     

一种茶叶鲜叶分级机的研制

在茶叶生产过程中,鲜叶原料分级是其中重要工序之一。当前,茶叶生产企业普遍采用传统滚筒式鲜叶分级机进行分级,对于机采作业而然,存在着鲜叶分级等次少、分级效果差、损伤率高、不方便收集等系列问题,难以适应机采鲜叶的分级要求。针对于这一情况,我们设计了一种新型螺旋导向鲜叶分级机构。本设计增加了筛筒长度,将鲜叶筛选分为四级,增加了分选等级;同时,通过筛筒内设置的双螺旋导向叶片的阻隔作用,延长筛选时间,提升筛选质量,通过改变筛筒形状结构,选用新型复合材料和不锈钢材料,降低机械对鲜叶的损伤率;其次,采用抽屉式的收集结构,更方便筛选后鲜叶的收集。此外,采用风机进行初步分选,减少了鲜叶分级后短碎叶含量,提升了后续分选效果,通过这一系列的改进设计,有望整体提升鲜叶筛选的效率及分级效果,从根本上解决机采鲜叶的分级问题。     

Phylogeography and Characteristics of Phosphorus Efficiency of Secondary Core Collection for Rice Landraces in Yunnan Province

[Objective] The paper was to explore the causes of phosphorus(P)efficiency of rice in Yunnan Province,and to provide strategy for ecological and environmental protection.[Method]Using 703 accessions of secondary core collections from 16 prefectures of five regions in Yunnan Province,two treatments of low available P(6.26mg/kg)versus normal P(available P 40mg/kg),and invalid P(available P 0.02 mg/kg)versus normal P(available P 70 mg/kg)were set,and zonal characteristics of phosphorus efficiency and the activation characteristics of invalid P of rice landraces were investigated.[Result] Phosphorus efficiency and the activation characteristics of invalid P in soils from rice landraces of Yunnan Province had the similar identification indexes,viz.the relative indexes of four traits(tillering ability or effective panicle,root weight,biomass,and straw weight)could be the screening indexes of gene type of secondary core collection with phosphorus efficiency,which also reflected the zonal characteristics of phosphorus efficiency and the activation characteristics of invalid P in soils of 16 prefectures among five regions.On the contrary,the relative panicle length,node length under panicle,leaf length,leaf width and plant height could only be the assistant indexes of identification for phosphorus efficiency.There were similar zonal characteristics between phosphorus efficiency and the activation characteristics of invalid P in soils of rice landraces in Yunnan Province.[Conclusion] The study had great importance to the conservation and utilization of biological diversity,which would make contribution to second green revolution of "less input,multiple output,promote health,and protect the environment".     

棉花诱导长柱头杂交制种及配套技术研究

旨在减少棉花杂交制种用工,提高棉花杂交制种工效,降低棉花杂交种子生产成本。采用GA₃溶液诱导棉花长柱头,不去雄直接授粉;采用小型强磁高速电动机作为动力,研制小型田间快速采粉器,同时研制与采粉器配套使用的简易授粉器。通过多年的研究,研制出一种新的杂交棉制种新方法,即用100 mg/kg浓度的GA₃溶液,处理8~18天母本的幼蕾,每花点滴量150~200 μL,开花当天,不去雄父本花粉直接授予母本伸长的柱头上;研制出与之相配套的棉花快速采粉器和低温贮能式授粉器,操作简便,田间采粉速度快,授粉效率高,生产的杂交种子纯度在95%以上,符合国家良种要求。该研究将稳定的诱导技术与采粉器和授粉器结合应用于棉花杂交制种,可节省大量用工,综合提高制种工效30%以上。