Quality of Mince from Alaska Pollock (Theragra chalcogramma) Frames

The quality and frozen stability of minces produced from Alaska pollock (Theragra chalcogramma) frames were compared against commercially produced fillet mince. Fresh frame mince had a higher defect level, lower nutritional content, and darker color. Cooked frame mince had a softer texture than commercial mince. After 6 mo frozen storage, frame mince had deteriorated to where it had unacceptable color, flavor and cooked texture. Rancidity increased more rapidly in frame mince than commercial fillet mince. Blending frame mince at levels of 10% to 20% with commercial mince produced acceptable fresh quality but marginal frozen storage stability.     

Host and environmental risk factors associated with Cryptosporidium scophthalmi (Apicomplexa) infection in cultured turbot, Psetta maxima (L.) (Pisces, Teleostei)

An epidemiological cohort study of Cryptosporidium scophthalmi in cultured turbot Psetta maxima L. of Northwestern Spain was conducted along a four-year period. Four different ongrowing cohorts were monitored monthly from introduction into the ongrowing tanks (10–50 g) until reaching market size (400–1400 g). The association of host and environmental factors with five categories of parasite abundance was assessed using a multivariable regression framework. Epidemiological factors assessed here were water temperature, weight, length, month of collection, season, age, origin, condition factor, water filtration, and status to the myxozoan Enteromyxum scophthalmi infection. E. scophthalmi was included into the analysis because it targets the same organ than C. scophthalmi and it was prevalent in the studied population. The multivariable analysis demonstrated the statistically significant association between several factors and parasite abundance. C. scophthalmi abundance was associated (P < 0.05) with age, condition factor, season, and status to E. scophthalmi infection. Young animals, with poor condition factor, during spring or summer, and not infected with the myxozoan were most likely to be highly infected by C. scophthalmi. Inclusion of these four variables significantly (P < 0.05) improved the model, compared to the model that did not include any of these epidemiological factors. Increasing levels of C. scophthalmi abundance were associated (P < 0.01) with higher severity of C. scophthalmi-compatible lesions. The frequency of distribution of C. scophthalmi abundance was clearly right-skewed and fitted a negative binomial distribution, whereas the intensity of infection fitted a Poisson distribution. The quantification of the variance-to-mean ratio stratified by age demonstrated overdispersion for 8–16 months old fish, although this bivariate association is likely affected by several other factors, as suggested by the results of the multivariable analysis. The negative relation between C. scophthalmi abundance and status to E. scophthalmi infection suggests differences in the transmission, onset, and course of both infections. The coarse filtration used in some cohorts did not significantly affect the levels of infection. C. scophthalmi was probably introduced into the ongrowing tanks mainly with carrier fish, though the involvement of infective oocysts from the water supply cannot be disregarded. Infection prevalence and mean intensity decreased with fish age and a seasonal distribution was found. Results presented here will help to understand the epidemiology of C. scophthalmi in turbot, to estimate the expected levels of infection associated with presence or absence of epidemiological factors, and to quantify the impact that the disease may have on susceptible turbot populations. The multivariable model used here is more powerful than the visual inspection of graphics for exploring associations in cooperative processes and can be easily extended to the assessment of epidemiological associations in other population and parasitic diseases.     

用熵权模糊综合评价法评价养殖池塘水质状况的研究

[目的]利用熵权模糊综合评价法对养殖池塘水质状况进行评价,以期能对池塘养殖的水质评判提供参考。[方法]利用熵权模糊综合评价法评价以乌克兰鳞鲤(Cyprinus carpio)为主养鱼,平均规格71.4 g/尾,密度分别为6 000、9 000、12 000尾/hm2的3组池塘的水质情况。[结果]根据我国地表水环境质量标准GB3838-2002,在养殖过程中,6 000尾/hm2组池塘属Ⅰ类水,9 000和12 000尾/hm2组池塘属Ⅴ类水。[结论]随着养殖密度的增大,池塘水质逐渐变差,但从综合评价结果也可看出9 000和12 000尾/hm2组池塘的水质没有差异。     

青岛市水产业经济增长方式转变研究

对青岛市水产业经济增长方式进行了分析,研究了制约其增长的因素,在此基础上提出了相应的建议,即通过水产品质量安全技术创新、水域生态安全技术创新、水产品良种繁育技术创新、完善水产业科技创新体系,大力发展休闲渔业等第三产业,实现青岛市水产业经济增长方式的转变。     

海水循环式养殖系统NH3—N,NO2—N转化及其水质管理

对海水循环式养殖系统NH_3－N、NO_2－N转化的研究表明,NO_2－N的降解转化是水质净化的关键因素。本文还讨论了生物负载、投饵、通气等管理行为对氯化合物转化的影响。     

Remediating sulfadimethoxine-contaminated aquaculture wastewater using ZVI-activated persulfate in a flow-through system

Antibiotic resistant bacteria can result from the improper discharge of water from the aquaculture farming industry. This calls for the development of a low-cost remediation technology. Our research verified the use of ZVI-activated persulfate (PS) to treat antibiotic-contaminated aquaculture discharge water in a flow-through system. We selected sulfadimethoxine (SDM) as a representative antibiotic residual and tested SDM degradation under varying parameters: activator dose, initial pH, chloride concentration, activator dose and timing. The results demonstrated that increasing the ZVI dosage significantly decreased SDM degradation due to the scavenging effects for the persulfate radical (SO₄⁻). SDM decomposition occurred when SO₄⁻ attacked the aniline moiety via electron transfer prior to undergoing hydrogen abstraction/addition on the sulfonamide. A high pH produced the fastest degradation with reaction rates following the order pH 11 > > pH 9 > pH 3 > pH 5. A high Cl⁻ concentration (>100 mM) enhanced SDM degradation because of the production of chlorine radicals. The experiment results from ZVI sequential addition indicated that only a small continual input of ZVI was sufficient to generate SO₄⁻ to react with SDM. We used a flow-through concept for the real discharge water that was spiked with SDM prior to treating with PS/ZVI. The results showed that our system was able to remove approximately 68% of SDM from filtered and 74% from unfiltered discharge water. These results provide proof-of-concept that our PS/ZVI system could potentially be developed to remediate antibiotic-contaminated aquaculture wastewater.     

水产养殖用蛭弧菌类生物制剂的检测

通过双层平板法以不同细菌为宿主测定了八个厂家蛭弧菌类生物制剂的噬斑含量及噬斑分离物的宿主裂解范围;以三对靶向不同类群蛭弧菌类生物的特异引物对各产品及其噬斑分离物进行了PCR检测;对两株代表性蛭弧菌分离物的16S rRNA基因进行了测序,并通过构建蛭弧菌科进化树分析了它们的系统地位。结果表明被检测的绝大多数蛭弧菌类生物制剂质量不合格,仅两个样品被蛭弧菌科特异引物检测为阳性,被双层平板法证实有噬斑出现。其中一个样品JSF中存在两种形态和宿主范围不同的噬斑分离物,且不同宿主菌计数结果存在明显差异。16S rRNA基因序列分析表明这两种噬斑分离物JSF1和JSF2,分别属于蛭弧菌科的噬菌蛭弧菌(类群1)和未定种的类群5。但另一样品的噬斑分离物则被证实为非蛭弧菌类生物。     

Probiotics in aquaculture: The need, principles and mechanisms of action and screening processes

Aquaculture production of molluscs is worth US$11 billion per year and represents 65% of World mollusc product. A significant limitation to the industry is loss of stock through bacterial disease. Traditional methods to combat disease with antibiotics have been questioned and alternatives have been sought. The field of probiotics as well as the screening methods used to acquire probiotic strains for the alternative management of disease in aquaculture is discussed. This review provides a comprehensive summary of probiotics in aquaculture with special reference to mollusc culture.     

Assessment of “discreet” vertebral abnormalities, bone mineralization and bone compactness in farmed rainbow trout

The present study aimed to evaluate the importance of “discreet” vertebral abnormalities in normally-shaped rainbow trout in relation to vertebral bone condition in French fish farms. A total of 373 trout (262 ± 2 mm in total length) from 20 fish farms sampled were studied. The fish were radiographed and the axial skeleton examined for vertebral abnormalities. Vertebrae from the middle axial region (V32-38) were selected to evaluate vertebral bone condition. Bone mineralization (BM, %) was estimated by the ratio of ash and dry weight. Bone compactness (BC,%) was measured using Bone Profiler 3.23 images software on digitized radiographs of transverse sections (125 ± 10 μm). Statistical analyses were performed to test the relationships between the occurrence of vertebral abnormalities, and BM and BC. The occurrence of affected trout ranged from 0 to 55% depending on the farm. Trout displayed vertebrae with low BM (< 54.6%) and low BC (< 28.1%) in 40% and 55% of the farm, respectively. No relationships were observed between bone condition parameters (BM, BC) and the occurrence of vertebral abnormalities. These results could be explained by a wide and variable plastic response of bone characters (i.e., vertebral abnormalities, BM and BC) to the various rearing conditions in the fish farms sampled.     

Optimal stocking in intensive aquaculture under sinusoidal temperature, price and marketing conditions

When the environmental and/or market conditions of an intensive aquacultural operation vary with time, maximization of profit may require a time dependent fish stocking rate. A simplified stocking problem is formulated where temperature, market price and/or market demand change sinusoidally over the annual cycle. The fish biomass sustaining-capacity is limited by the water treatment equipment, and is expressed in terms of maximum feeding rate per unit volume of culture tanks.It is demonstrated that under such conditions, sinusoidal stocking rates produce good sub-optimal solutions. A critical element of the solution is the time delay (phase-shift) between the constraining (restricting) conditions (temperature and market), and the stocking cycle. Furthermore, if the solutions for two individual conditions have very different time delays, a combination of the two conflicting restrictions is likely to produce poor returns.