ALV-J人工感染鸡病毒血症和抗体反应动态

 【目的】了解J亚群白血病病毒（ALV-J）感染鸡后抗体反应和带毒排毒的规律,以便为制定鸡群中 ALV-J感染的预防控制和净化措施提供可靠的流行病学依据。【方法】1日龄和49日龄SPF鸡分别通过注射、口服和接触感染ALV-J,对各感染组的病毒血症、泄殖腔排毒及抗体反应进行动态检测。【结果】不同日龄感染ALV-J后的病毒血症和抗体的动态有很大差异。1日龄SPF鸡注射感染ALV-J后,自2周起开始可检出泄殖腔p27抗原和病毒血症。注射感染组有71%（5/7）鸡在26周内持续带毒排毒,其中有3只鸡在感染后45周仍带毒排毒。其中一只鸡在16周时产生一过性抗体,另两只鸡完全没有抗体。口服感染组仅11%（1/9）鸡呈持续带毒排毒,44%（4/9)鸡仅呈短暂性带毒排毒,且自第8周起即可检测到较高的抗体水平。同笼接触组既不带毒排毒,也无抗体水平,表明未发生传染。49日龄SPF鸡即使注射攻毒后,在19周内的5次检测中,p27抗原和病毒血症均为阴性。攻毒后1周开始出现抗体反应并维持一段时间。49日龄SPF鸡经口服和接触感染,病毒血症和泄殖腔p27均为阴性,也无抗体反应。【结论】1日龄SPF鸡感染ALV-J很容易发生免疫耐受,感染鸡持续带毒排毒而不产生抗体;49日龄SPF鸡感染后,1周可产生高水平的抗体,但不带毒排毒。不同接种方式对ALV-J感染的动态有很大影响,注射感染诱发的病毒血症和耐受性病毒血症显著高于口服感染。     

甜菊新品系83-27的栽培技术

从国内外引进的不同来源品种群体中,选择变异植株进行糖甙含量分析,经过6年的系统选育,选育出了抗性强、产量高、含糖甙高的甜菊新品系83-27。该品系与生产用种比可增产10%～15%,含糖甙比生产种高3%以上。本文从播种育苗、苗田管理、移栽田管理及收获与采种等方面详细介绍了甜菊83-27品系的栽培技术。     

cDNA cloning of goldfish Hsp27 and its chaperone activity

A cDNA fragment encoding goldfish Hsp27 was amplified by polymerase chain reaction and its nucleotide sequence was determined. The deduced amino-acid sequence of goldfish Hsp27 showed 58–62% identity to Hsp27 from other vertebrates. Three serine residues reported to be phosphorylated in human Hsp27 were conserved in goldfish Hsp27. The Hsp27 gene was transcribed in goldfish culture cells after temperature shift from 20°C to 40°C, but not from 20°C to 35°C. Recombinant goldfish Hsp27 was examined for its in vitro chaperone activity and compared to those of Hsp30 from the same fish and human recombinant Hsp27. Goldfish Hsp27 and Hsp30 showed a similar activity at 10 μM, which was significantly lower than that of human Hsp27. At 1 and 5 μM concentrations, however, goldfish Hsp27 showed slightly lower activity than goldfish Hsp30, but the activities of both goldfish proteins still remained significantly lower than that of human Hsp27. Meanwhile, goldfish Hsp27 formed oligomer, which was slightly smaller than that of Hsp30. These results suggest that goldfish Hsp27 contributes as a molecular chaperone in association with Hsp30 to compensate for stress resulting from rapid temperature fluctuations. The nucleotide sequence reported in this paper has been registered into the DDBJ/EMBL/GenBank databases with accession number AB239443 for goldfish Hsp27.     

禽白血病病毒p27蛋白在大肠杆菌中的表达和多克隆抗体的制备

将禽白血病病毒p27基因克隆并构建重组表达质粒pET28a-p27,在大肠杆菌BL21中经IP TG诱导后产生可溶性表达蛋白。表达的蛋白用Western blot进行活性检测,其能与辣根过氧化物酶标记的兔抗p27抗体发生特异性反应;采用金属螯和层析对表达蛋白进行纯化,其纯度约为95％;用纯化的表达蛋白p27免疫家兔制备抗血清,ELISA抗体效价可达1∶25600。研究结果表明,大肠杆菌表达的p27蛋白具有良好的抗原性,可以替代纯化病毒蛋白用于禽白血病病毒检测。     

抗锈高产种质汕油27在花生育种上的应用

 汕油27是汕头市农业科学研究所育成的中抗锈病、高产稳产、适应性广的花生新品种，由该品种衍生的许多品种在不同时期成为中国南方花生产区的当家种或推广良种。为了了解该优良种质在花生新品种选育中的作用，剖析了以汕油27作为直接或间接亲本选育的后代优良品种的系谱、遗传特性以及与汕油27的遗传关系，结果表明，由汕油27作为直接亲本先后衍生出优良花生新品种5个，进而由这些品种又衍生出的通过审定（或在省级区试中表现较突出）的花生新品种17个；在这22个优良衍生品种中，通过国家审定的有6个，包括粤油223、粤油79、粤油7号、粤油9号、汕油21和湛油30；所有衍生品种都保持了汕油27高产、抗锈病等优良特性。因此，作者认为汕油27是一个一般配合力较高的优良中心亲本；选准中心亲本对提高花生育种效率具有十分重要的作用。     

抗条锈冬小麦新品种兰天27号选育报告

冬小麦品种兰天27号以法国抗源材料Fr81-1为母本,兰天10号为父本杂交选育而成.2007-2008年甘肃省陇南片山地组区试结果,10点（次）平均折合产量6235.5 kg/hm2,较对照品种中梁22号增产22.66％.该品种冬性,属弱筋小麦.接种鉴定表现对当前主要流行条锈病生理小种全部免疫,适种区为甘肃天水、陇南两...     

家蚕27、28号染色体基因的生物信息学分析

利用家蚕基因组精细图分析第27、28号染色体上的基因结构及其编码蛋白的功能结构域,可为在家蚕资源库中鉴定这两条染色体上的突变基因以及发现新的形态标记提供重要线索。基因预测及结构分析显示:家蚕第27和28号染色体是基因数目最少的两条染色体,预测基因数目分别是241个和288个,平均每个基因分别具有7.7和5.3个外显子;蛋白质全长编码区序列(CDS)长度主要分布于400-800 bp之间;CDS的GC含量分别是49.72%和46.54%。蛋白质功能结构域分析发现,家蚕第27、28号染色体上基因所编码的蛋白分别具有76和92种结构域,其中最多的3种结构域分别为Sugar_tr、MFS_1、zf-C2H2和UDPGT、DUF、Serpin。将家蚕第27、28号染色体上的预测基因与果蝇突变基因进行同源检索的结果显示,家蚕第27、28号染色体上分别有16和15个基因具有明显的可能突变表型。     

Effect of Different Test Materials on the Detected Results of Avian Leukosis Virus-p27 Antigen by ELISA

In order to study the effect of different test materials on the detected results of avian leukosis virus (ALV)-p27 antigen by ELISA, the cloacal swabs ALV-p27 antigen were detected by ELISA in 180-day local breeds hen, and the parts of the positive and negative chicken was selected to group test, the serum, egg whites and cell supernatant ALV-p27 antigen were detected by ELISA, the specific genes of ALV were detected by RT-PCR in blood.The results showed that serum, egg white and cell supernatant as ELISA test materials, the positive rate lower of than cloacal swabs, and serum ALV-p27 positive samples include all egg whites and cell supernatant positive samples in positive group.It was a significant correlation between ELISAs with serum and cell supernatant (linear equation:Y=1.8439X-0.1469, the correl was 0.937).In positive group, ALV-p27 gene positive rate lower than cloacal swabs ELISA, but higher than the serum, egg and cell culture medium, and ALV-p27 gene positive samples include serum positive samples by ELISA.ALV-J gp85 gene positive rate of 29.17%, and all positive samples were included in the serum ALV-p27 positive samples.The results suggested that the cloacal swabs as test material may occur false positive results, and egg whites and cell supernatant may occur undetected by ELISA ALV-p27 antigen assay in adult chicken, serum as test material in ELISA could more accurately reflect the state of adult chickens infected with ALV.     

MGSC27微生物肥料对土壤作用的研究

在实验室条件下对ＭＧＳＣ２７微生物肥料施用于土壤的效果进行了研究,结果表明：ＭＧＳＣ２７微生物肥料能够提高土壤的全Ｎ、速效Ｐ、速效Ｋ含量,其微生物能够较长时期地存在于土壤中。     

云南省昆明地区种鸡J亚群禽白血病血清学调查

[目的]了解云南省昆明地区种鸡场J亚群禽白血病的感染状况。[方法]从云南省昆明市疑似感染禽白血病的某种鸡场采集644份泄殖腔(公鸡)拭子和460份蛋清(母鸡)样品,采用ELISA法对种鸡J亚群禽白血病进行抗原检测。[结果]在检测的460份蛋清样品中,172份抗原呈阳性,阳性率为37.39%;在检测的644份泄殖腔拭子中,520份抗原呈阳性,阳性率为80.75%。A养殖场共检测644份样品,总阳性率为63.66%,其中蛋清样品的阳性率为39.13%,泄殖腔样品的阳性率为82.07%。B养殖场共检测460份样品,总阳性率为61.30%,其中蛋清样品的阳性率为34.78%,泄殖腔样品的阳性率为78.99%。此次检测结果表明云南省昆明地区种鸡群已严重感染J亚群禽白血病。[结论]该研究可为种鸡J亚群禽白血病的防治提供理论依据。