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101.
Liver lesions were found in 6/6 pigs 7 months after oral inoculation with 5000 or 35,000 Echinococcus multilocularis eggs. However, lesion morphology differed considerably among the animals. The largest lesions (3–8 mm in diameter) were found in a single pig and smaller lesions (1.5–3 mm) in three pigs. These lesions were clearly circumscribed and had pronounced central necroses and dystrophic calcifications. In contrast, most of the smallest (usually <1.5 mm in diameter) found in two other pigs, had small compact fibrotic areas and blurred borders with obvious fibrous infiltrations into the interlobular tissues. E. multilocularis specific DNA was detected by PCR in all lesion types, but metacestode viability, as assessed by in vivo intraperitoneal inoculations in jirds, could not be demonstrated. Within 1 month post inoculation, all pigs developed specific IgG antibody responses against a battery of different antigens (metacestode, cyst fluid, and protoscoleces-derived native E. multilocularis and E. granulosus antigens, affinity purified Em2G11 antigen, antigen B, recombinant Em II/3–10 antigen). Two different reaction patterns were recorded. In the two pigs with the small lesions, pronounced reactions against all crude antigens with peaks 3–5 months p.i. and clearly elevated levels until the end of the experiment were noted. In all other pigs, antibody reactions remained low in all cases. In conclusion, we demonstrated two types of E. multilocularis metacestode development in pigs with distinct immunological response patterns. 相似文献
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蠡玉16玉米受旱减产规律及产量与水关系模型研究 总被引:1,自引:0,他引:1
以淮北平原地区常用玉米品种蠡玉16为试验材料,采用测坑法进行作物的受旱试验,研究主要农作物不同受旱条件下的减产规律,构建产量与水关系模型。结果表明,蠡玉16玉米关键期受旱对其产量的影响十分显著,轻旱、中旱、重旱的减产率分别为4.3%、36.8%、51.1%,且随着受旱程度的增大减产率剧增;蠡玉16玉米在抽雄灌浆期对水分亏缺最为敏感,敏感指数达0.925 1;其次是拔节期、分蘖期和乳熟期,敏感指数分别为0.126 2、0.066 2和0.003 2。水分亏缺对蠡玉16玉米产量影响最大阶段为抽雄灌浆期,同时也是需水强度最大期。 相似文献
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This paper presents the development of a simple and generally applicable hydrodynamic method for the estimation of production carrying capacity (PCC) of coastal finfish cage aquaculture. Dimensional analysis was used to find significant and general interdependencies between the hydrodynamics at fish farm locations and particulate wastes deposited on the seafloor by fish farms. Modeled ratios of deposition to emission of particulate wastes underneath fish farms were found to be primarily a function of the flow Reynolds numbers at the farming locations and the non-dimensional settling velocity of emitted wastes. In the non-dimensional model, farming conditions include daily feed rate, proportion of unconsumed feed, and carbon content in feed and fish feces. The relationship can be used to estimate the PCC of floating net cages imposing a threshold value for deposition. Results of in-situ assessments of the benthic impacts of several fish farms in an aquaculture site in the northwest of Bali, Indonesia were used to validate and demonstrate the effectiveness of the method. Predicted results were able to clearly identify fish farms operating beyond ecologically sustainable carrying capacity. The proposed method has broad applicability and could help make decisions regarding the estimation of production potential of individual farms in pristine areas, for providing first estimates in sites that have scarce data, and for assessment, expansion, and optimization of the currently operating aquaculture sites in Southeast Asia, China and potentially other data-poor island nations. As the method relies on dynamic models, it enables straightforward assessments over the entire aquaculture region. 相似文献
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