不同豆类对绿豆象发育繁殖的影响

为给综合治理绿豆象提供理论支撑,在室内(28±2℃)条件下,饲养绿豆象,并观察了6种豆类对绿豆象发育和繁殖的影响。结果表明:绿豆象的卵孵化后,幼虫仅在绿豆、红小豆、大豆上可蛀入危害;在绿豆、红小豆和大豆上的世代发育历期分别为26.50、29.90、52.00天;在绿豆、红小豆、大豆上的存活率分别为52.11%、56.45%、1.60%;绿豆象成虫在不同豆种上的产卵历期差异不显著(P0.05);但雌成虫在不同豆种上的产卵量差异显著(P0.05)。综上认为绿豆象的发育和繁殖明显受储粮豆类的影响。     

Spontaneous well-differentiated pancreatic islet cell carcinoma with vascular invasion in a male F344 rat

This case report describes a case of spontaneous pancreatic islet cell carcinoma with vascular invasion in a 110-week-old male F344 rat. Histologically, a pancreatic nodule consisting of tumor cells and many blood-rich vessels, and covered with a fibrous capsule showed local invasion in the capsule and adjacent acinar tissues, encircling a large duct-like structure (DS). The tumor was composed of well-differentiated tumor cells resembling normal pancreatic islet cells, which had small round nuclei and eosinophilic cytoplasm. Mitotic figures were rare. Immunohistochemistry revealed that the tumor cells were positive for insulin. Although endothelial cells were not detected, the DS wall showed cells positive for α-smooth muscle actin and elastic fibers, suggesting that the DS is the pancreatic artery. This is a rare case of islet cell carcinoma consisting of well-differentiated tumor cells with invasion of the pancreatic artery in a rat.     

Malignant pinealoma observed in the deep cerebral parenchyma of a male Wistar rat

This report describes a case of spontaneous malignant pinealoma in a 90-week-old male Wistar rat. The tumor mass occurred in the deep cerebral parenchyma and no intact pineal gland was observed in the area between the posterior-dorsal median line of the cerebrum and the cerebellum. The tumor was characterized by a large nodular proliferation occupying the central area of the brain, extending from the dorsal surface to the base of the brain, corresponding to the thalamus. The tumor cells had round to irregular oblong nuclei approximately 5–17 μm in diameter and showed faintly or moderately eosinophilic cytoplasm and indistinct cell boundaries. Immunohistochemically, the tumor cells were positive for synaptophysin and partially positive for neuron-specific enolase (NSE). The tumor showed malignant features including cellular pleomorphism, high mitotic index, necrotic foci, and invasive and extensive growth and was, therefore, diagnosed as an extremely rare malignant pinealoma in the deep cerebral parenchyma.     

A possible critical dosing period of p-cumylphenol for development of cystic kidneys in rat neonates

In accordance with a previous report on cystic kidneys induced in rat neonates when dosed with p-cumylphenol (PCP) for 18 days from postnatal day (PND) 4, 3 rat neonates were dosed with PCP once a day for 14 days, either from PND 14, 21, 28, 35, or 42 as W2, W3, W4, W5, and W6 groups, respectively, to investigate whether dosing periods in different PNDs influenced the development of cystic renal tubules. The lesion was striking in the W2 group and at a lesser magnitude in the W3 group, whereas either kidney was unaffected when dosing was initiated beyond PND 28. These findings, together with the results from the previous study, suggested that PND 14-28 is a critical dosing period for PCP to develop cystic kidneys in rat neonates. The lining epithelium of the cystic tubules was immunohistochemically positive for AQP2. This finding and the anatomical location indicated that the cystic tubules were of collecting duct origin. Either obstruction, fluid accumulation, or reparative hyperplasia of the lining epithelium was unlikely to be involved in the formation of cystic tubules lined with a monolayer of cuboidal or columnar epithelium with a high nuclear density. Thus, the follow-up investigation on PCP suggested a critical dosing period of PND 14-28 in rat neonates for the development of cystic dilation of renal collecting ducts. This study further supports that additive hyperplasia of the lining epithelium is a fundamental basis of this unique lesion.     

大鼠发情周期各阶段的阴道细胞变化观察

为了比较准确的确定大鼠发情周期各个阶段,利用阴道组织涂片法,对14只6月龄雌性Wistar大鼠连续20 d进行阴道涂片,镜检,观察阴道涂片中细胞的变化规律。结果表明,在发情间期,阴道涂片以白细胞为主(84.6个±1.1个);发情前期以有核上皮细胞为主(72.4个±8.3个);在发情期,以角质化的上皮细胞为主(78个±5.4个);发情后期角质化上皮细胞、有核上皮细胞和白细胞3种细胞都有,而且比例相当。阴道涂片法能简便有效的区分大鼠发情周期的各个阶段。     

提高无性系茶苗定植成活率的技术措施

无性系茶苗定植后成活率低,是当前制约无性系茶园建设的主要因素.本文通过大量调查分析,找出了影响无性系茶苗定植成活率的主要因素,探讨了提高无性系茶苗移栽成活率的措施.     

利用秋水仙素喷淋加倍不同遗传基础的单倍体子粒探讨

以不同血缘及不同世代的遗传育种材料为母本、高频孤雌生殖诱导系辽诱1和CAU5等为父本杂交诱导产生的单倍体子粒为实验材料,研究秋水仙素浓度为0.625 mg/mL的条件下,用喷淋加倍设备对试验材料进行8 h喷淋的加倍效果。结果表明,喷淋秋水仙素后,郑单958的S2代的材料加倍率最高,为33.75%;第2位的是L3258×H71,是NSS种质S0代材料,其加倍率为13.02%;第3位的是D299/PH4CV,为NSS种质S1代材料,加倍率为10.34%。研究表明,喷淋秋水仙素的加倍效果与遗传背景关系紧密,喷淋秋水仙素加倍率总体由高到低的顺序为郑单958NSS种质(SS+NSS)种质SS种质欧洲杂交种,喷淋秋水仙素对全部试验材料的加倍率都有提高作用,比自然加倍提高0.83～5.08倍。利用该喷淋设备加倍单倍体子粒,具有节约用药量、操作简便、便于大规模生产应用、减少人员对秋水仙素的接触、废液回收方便、有利于保护环境等优点。     

Effects of Different Processed Products of Radix Codonopsis on Intestinal Flora of Rats with Spleen Deficiency

[Objectives]This study aimed to observe the effects of different processed products of Radix Codonopsis on intestinal flora in rats with spleen deficiency.[Meth...     

槲皮素对运动训练大鼠能源物质代谢的影响

以跑台训练建立动物模型,通过测定运动训练大鼠血糖、血浆游离脂肪酸(FFA)、血尿素氮(BUN)、血清胰高血糖素、生长激素含量和大鼠力竭运动时间,评价抗氧化剂槲皮素对运动大鼠运动能力的影响。结果表明,服用槲皮素后大鼠力竭运动时间明显延长,力竭即刻,槲皮素力竭组较力竭对照组大鼠BUN含量显著降低,而血糖、血浆FFA、血清胰高血糖素、生长激素含量显著提高。提示槲皮素可通过影响激素分泌,促进脂肪动员而保持血糖、BUN含量的相对稳定,从而提高大鼠的运动能力,槲皮素可作为一种运动保健品开发利用。     

高脂饮食对大鼠动脉血管张力的影响

目的：观察高脂饮食大鼠动脉血管张力的改变及内源性ＮＯ、ＰＧＩ２的作用。方法：ＳＤ大鼠１６只分为高脂饮食组和对照组,喂养５周后测定血清血脂浓度和胸主动脉环对云甲肾上腺素（ＮＥ,１０＾－８￣１０＾－４ｍｍｏｌ／Ｌ）、乙酰胆碱（ＡＣｈ,１０＾－８￣１０＾－４ｍｍｏｌ／Ｌ）的反应。结果：与对照组比较,高脂饮食组ＴＣ、ＬＤＬ明显升高（Ｐ〈０．０１和Ｐ〈０．０５）;胸主动脉环对ＮＥ或ＡＣｈ的反应明显减弱（Ｐ〈