磁共振常规及弥散加权成像对预测宫颈癌淋巴结转移的价值

目的 探讨磁共振常规及弥散加权成像在宫颈癌淋巴结转移诊断中的敏感性、特异性.方法 113例ⅠB1至ⅡB期宫颈癌患者行术前MRI常规及弥散加权成像检查、根治性子宫切除术加盆腔或盆腔及腹主动脉旁淋巴结清扫术.结果 94例(83.2%)经术后病理学检查证实无淋巴结转移,19例(16.8%)有淋巴结转移.MRI常规及弥散加权成...     

棉花茎枝叶形态模型研究

 在2005－2006年盆栽试验基础上,系统分析了生态因子对棉花主茎叶、果枝叶和主茎节间、果节形态发生的影响,量化了温度、氮素、水分、化控（DPC）等与棉花各器官形态建成的关系,构建了基于有效积温（GDD）、以Logistic模型为基础的棉花主茎和果枝的叶片长度、宽度、叶柄长度及主茎节间、果节长度和粗度形态发生的动态模型。利用不同氮素水平、不同品种、水分、化控试验资料对模型进行了检验。结果表明,棉花主茎和果枝的叶片长度和宽度、叶柄长度及主茎节间、果节长度和粗度的模拟值与观察值之间均方差根（RMSE）分别为0.66 cm、0.87 cm、0.77 cm、0.57 cm、0.77 mm、0.43 cm、0.55 cm、0.43 cm、0.73 cm、0.56 mm,棉花器官形态发生的模拟值与观测值具有较好的吻合度,说明模型具有较好的预测性和准确性     

有限元法在材料力学中的应用

有限元法在工程技术的领域得到了广泛的应用,它是一种利用固体理论基础的数值分析方法,本文主要探讨有限元法在材料力学中的应用。     

基于引力模型的丝绸之路经济带甘肃段节点城市空间发展战略

推进丝绸之路经济带建设是中国西北地区开发开放、转型发展的重大机遇,明确各省、市空间发展定位,避免同质化竞争是国家战略稳步实施的关键。以丝绸之路经济带甘肃段各节点城市为研究样本,通过主成分分析测算样本城市综合质量,在明确核心节点城市的基础上修正研究模型计算得出的各节点城市间的引力值和作用场强。结果显示:丝绸之路经济带甘肃段各节点城市在发展过程中组团化与空间分异化特征并存,兰州是丝绸之路经济带甘肃段的核心城市,同周边城市联系紧密、融合互动发展能力强。嘉峪关城市发展存在偏心化,发展重心向酒泉转移。酒泉城市综合质量较高,具备带动周边城市发展的能力。张掖经济发展模式单一,区域协同发展基础薄弱,亟需提高城市引力,与武威、金昌等城市实现联动。庆阳、平凉具备实现孤岛城市和孤点城市向龙头和龙尾城市转变的基础。陇南城市综合质量低,城市引力与场强不足,需要联系周边城市协同发展。临夏、甘南作为少数民族地区,需提升城市发展水平,摆脱孤点城市困局。最后,针对研究结果就丝绸之路经济带甘肃段节点城市空间差异进行梳理,并就各自发展策略提出相关建议。     

Seaweed polysaccharide mitigates intestinal barrier dysfunction induced by enterotoxigenic Escherichia coli through NF-κB pathway suppression in porcine intestinal epithelial cells

This study aimed to investigate the protective effects and underlying mechanism of seaweed polysaccharide (SWP) on intestinal epithelial barrier dysfunction induced by E. coli in an IPEC-J2 model. A preliminary study was done to screen optimum SWP concentrations by cell viability, cytotoxicity, apoptosis and proliferation evaluation. The regular study was conducted to evaluate the protective effects of SWP against E. coli challenge via the analysis of transepithelial electrical resistance (TEER), tight junction proteins, NF-κB signalling pathway, proinflammatory cytokines and the E. coli adhesion and invasion. Our results show that 4 h E. coli challenge down-regulated tight junction proteins expression, decreased TEER, activated NF-κB signalling pathway and increased proinflammatory response, which indicates that the E. coli infection model was well-established. Pre-treatment with 240 μg/ml SWP for 24 h alleviated the 4 h E. coli -induced intestinal epithelial barrier dysfunction, as evidenced by the up-regulated expression of Occludin, Claudin-1 and ZO-1 at both mRNA and protein level and the increased TEER of IPEC-J2 cells. Pre-incubation with 240 μg/ml SWP for 24 h inhibited the activation of the NF-κB signalling pathway by 4 h E. coli challenge, including the decreased mRNA expression of TLR-4, MyD88, IκBα, p-65, as well as the reduced ratio of protein expression of p-p65/p65. Also, pre-treatment with 240 μg/ml SWP for 24 h decreased proinflammatory response (IL-6 and TNF-α) induced by 4 h E. coli challenge and decreased the E. coli adhesion and invasion. In conclusion, SWP mitigated intestinal barrier dysfunction caused by E. coli through NF-κB pathway in IPEC-J2 cells and 240 μg/ml SWP exhibited better effect. Our results also provide a fundamental basis for SWP in reducing post-weaning diarrhoea of weaned piglets, especially under E. coli -infected or in-feed antibiotic-free conditions.     

毛竹节间材与节部材的构造与强度差异研究

竹节是竹子为适应其生存环境长期择优演化而形成的特殊构造,它对提高细长竹子的刚度与稳定性至关重要。但在竹的利用中,竹节对竹材的力学强度是否会有影响,说法不一。现以毛竹为研究对象,在比较了竹节间材和竹节之间的构造差异的基础上,以竹青、竹黄、竹节未处理和已刨平处理的两种试件形式,分析研究了毛竹的含节材与不含节的节间材在强度上的差异。结果表明,无论是自然状态下的竹材试件,还是刨去刨平青、黄、节的竹材试件,竹节对弯曲强度、顺纹剪切强度、抗压强度等力学性能均未见有降低的作用,相反还有着不同程度的增强作用。但竹节对竹材顺纹抗拉强度有着不可忽视的降低影响。     

留瓜节位对厚皮甜瓜植株生理特性、果重及可溶性固形物含量的影响

以塑料大棚栽培的厚皮甜瓜品种‘翠雪5号’为试验材料,探讨不同留瓜节位对不同节位叶片的生理生化指标、单果重以及可溶性固形物含量的影响。结果表明,‘翠雪5号’在第16节位留瓜时,可溶性蛋白（Pr）含量和叶绿素（Chl）高于第13节位留瓜,丙二醛含量（MDA）和愈创木酚过氧化物酶（POD）活性低于第13节位留瓜。然而,不同留瓜节位还会影响甜瓜植株衰老进程,对果实产量和品质有着重要的影响。一般来说,同一个品种春季栽培比秋季栽培的坐瓜节位要高2个节位左右,这样结出的瓜不仅果形好且大、产量高,糖度也高。     

Influence of ischemia-reperfusion on apoptosis of sinoatrial node cells in rabbits in vivo

AIM:To study influence of ischemia-reperfusion(IR) on apoptosis and expression of apoptosis-related genes Fas-L, Bax and Bcl-2 of sinoatrial node(SAN) cells in rabbits in vivo. METHODS:Ninety healthy adult rabbits were divided randomly into control group, ischemia groups (I_{10 min}, I_{30 min}, I_{60 min} and I_{120 min}) and IR groups (I_{10 min}R_4h, I_{30 min}R_4h, I_{60 min}R_4hand I_{120 min}R_4h). IR injury model of SAN was established by occluding and loosening the start section of right coronary artery. The apoptosis of SAN cells was detected by TUNEL staining. The expression of Fas-L, Bax and Bcl-2 of SAN cells was detected by immunohistochemistry. RESULTS:①No obvious apoptosis of SAN cells was observed in control group, I_{10 min} and I_{30 min} groups. Apoptosis of different degrees in SAN cells were found in 68.3%(41/60) rabbits in I_{60 min}, I_{120 min} and 4 subgroups of IR. ②The highest expression of Fas-L and Bax was observed in I_{120 min} group and that of Bcl-2 was in I_{60 min} group. ③The highest expression of Fas-L and Bax was observed in I_{60 min}R_4h group. The peak level of Bcl-2 was observed in I_{30 min}R_4h group. ④The expression of Fas-L and Bax was significant higher in IR group than that in ischemic group at the same time point. CONCLUSION:Ischemia and IR induced apoptosis of SAN cells in rabbit in vivo. Fas-L、Bax、Bcl-2 may participate in the regulation of apoptosis and the injury during IR aggravates the apoptosis of SAN cells.