Sequence analysis and prokaryotic expression system construction of PIA genes isolated from Neisseria gonorrhoeae

AIM: To analyze the nucleotide and putative amino acid sequences of PIA genes isolated from N.gonorrhoeae and to construct the prokaryotic expression system of PIA gene.METHODS: The entire PIA genes from 9 strains of N.gonorrhoeae were amplified by using high fidelity PCR.The target amplification fragments were sequenced after T-A cloning.Homology comparison of the nucleotide and putative amino acid sequences of PIA genes from the isolates with the reported sequences in GenBank was then performed.A prokaryotic expression system of PIA gene was constructed.Different dosages of IPTG were applied to induce the expression of the target recombinant protein (rPIA) and 10% SDS-PAGE plus Bio-Rad Agarose Image Analysor was used to determine the expression level of rPIA.rPIA was extracted using Ni-NTA affinity chromatography and the purified effect was detected by SDS-PAGE.RESULTS: In comparison with the reported PIA gene sequences (GenBank No: L19962),the homologies of nucleotide and putative amino acid sequences of PIA genes from the isolates were 99.6%-100% and 99.1%-100%,respectively,which indicated that all the isolates were belonging to serovars IA6.Output of rPIA was as high as 50.1% of the total bacterial proteins.The purified rPIA only showed a single target protein fragment in gel.CONCLUSION: Serovar IA6 is dominant in the local N.gonorrhoeae isolates and sequences of the encoding gene are relatively conserved.The constructed prokaryotic expression system is able to express rPIA with high efficiency,which may lay a foundation for further development of serological detection kit and vaccine of N.gonorrhoeae.     

Genetic Variance and Covariance Patterns of Growth and Survival in Northern Pinus sylvestris

The amount of additive genetic and environmental variance for tree height and field survival, and the genetic relationship between the traits were estimated using data from half-sib progenies of Swedish and Finnish Scots pine (Pinus sylvestris L.) plus-trees, assessed in 18 single-tree plot progeny trials. The progeny trials were established in northern Sweden and comprised 9-13-yr-old, Finnish polycross progenies or Swedish open-pollinated progenies. In total across the trials, 71?630 individual trees from 888 families were included in the study. At the overall level, the additive genetic coefficient of variation ranged between 3.1 and 16.3% for height and between 0 and 27.9% for survival, with averages of 9.5% and 14.2%, respectively. Narrow-sense heritabilities were moderate to low, with averages across trials of 0.11 for height and 0.06 for survival. At the within-population level, estimates of genetic correlation between height and survival were mostly large and positive, with an arithmetic mean and standard error across trials of 0.47±0.39.     

杉木树干材积分析

就杉木人工林立木树干材积的估测，材积按树高分布，材积的组成等有关问题进行了探讨。     

基于村民委员会候选人产生方式的调查与分析

文章以L村和X村村民委员会换届选举中村委会候选人的产生方式为例,指出在村民委员会候选人确立方式上存在着行政权力与自治权之间的冲突,分析原因并提出解决路径,即在立法方面明确村民自治的内函与地位,行政部门应充分为村民自治创造空间,尝试无候选人选举等。     

晋大62×诱处4号杂交后代群体遗传多样性分析

选用晋大62×诱处4号杂交亲本和后代群体为试验材料,通过微卫星(SSR)标记分析,利用SPSS软件对其进行了聚类分析,探讨了亲本及后代群体间的遗传特性和亲缘关系,研究了大豆群体的遗传多样性,为大豆杂交育种亲本的选配、杂交后代的筛选、种质创新及大豆遗传多样性研究提供了理论和实践依据。结果表明,以亲本的基因组DNA为模板,选用40对不同的引物进行电泳共筛选出23对位于多条染色体上的差异引物,其多态性比例为55.0%。通过聚类分析所有材料可聚类为以下几类:母本晋大62(CK1)和后代6~13号材料聚为第一大类,父本诱处4号(CK2)和后代25~31号材料聚为第二大类,而1~5、14~20聚为第一小类,21~24、32~35聚为第二小类,这两小类材料与亲本的亲缘关系较远。     

新型城镇化战略下生活体育设施建设的思考——基于CiteSpace可视化分析

新型城镇化包含了以人为本以及通过不断改善民生提高城镇居民生活满意度两方面的战略思想。生 活体育作为新型城镇化中精神文明建设的一个方面，不仅是促进新型城镇化发展的重要手段，也体现着新 型城镇化发展的重要价值。通过发展生活体育，新型城镇化将以提高居民生活质量为目标，提高居民身体 素质的同时提高居民生活满意度。通过CiteSpace可视化分析发现，国内对生活体育研究还处于主题分散 ，框架不明晰的阶段。将在新型城镇化战略指导下，通过搭建生活体育设施建设框架，不仅可以弥补体育 手段和生活体育理论联系的不足，而且可以弥补生活体育设施建设研究领域中缺乏的相关文献回顾，同时 为未来生活体育设施建设提供了理论指导模型。     

华东监测区营造林与人工林质量状况评价

选取了华东监测区近三年营造林综合核查和全国第六次森林资源连续清查的相关数据。对华东监测区八省（市）营造林质量主要指标进行了分析。运用SPSS软件对华东监测区营造林质量与人工林质量的有关指标进行了聚类分析。按照聚类分析结果对华东监测区八省（市）营造林质量、人工造林与人工林质量分成三种类型。     

桔梗单倍体染色体核型分析

首次分析了桔梗单倍体染色体核型，并与已报道的二倍体桔梗染色体核型分析资料进行比较，对它们的关系进行了初步探讨。它全由中部和近中部着丝点染色体组成，第6号染色体具随体，核型公式为K（n）=X=7m（1SAT）＋2sm属“1A”类型。     

CNMARC著录中1XX字段的著录分析

结合实例就IXX字段著录过程中容易出现混淆的代码进行分析，提高CNMARC记录中1XX字段的录入质量。     

动物家谱分析系统的研制与开发

本文论述了动物家族系谱分析系统的组成及其功能，采用线条图的形式来描绘动物各代之间的血缘关系，通过对动物个体在家族中的特性作用的统计分析，将为动物养殖繁育提供重要的参考价值。