大孔树脂吸附及超滤膜技术提纯蓝莓花色苷中试研究

比较了4种大孔吸附树脂的吸附和解析能力,确定生产最适合的树脂;分析了温度、pH值、洗脱剂浓度对树脂吸附的影响,确定最有利于产业化的工艺参数;通过不同压力、截留分子量比较超滤结果,选择最优化的超滤工艺参数。试验结果表明：在4种树脂中,D101树脂具有最佳的吸附性能;采用D101大孔树脂和6000道尔顿分子量的超滤膜纯化技术对野生蓝莓花青素纯化效果最佳,花青素纯度可达35％以上。     

Samenkonservierung und hormonelle Beeinflussung der Samenqualitat beim Kamel

Inhalt: Nach kuner Beschreibung des Eintritts der Geschlechtsrege beim Kamel wird auf die Samengewinnung, sowie die Flüssig- und Tiefgefrierkonservierung des Samens einge-gangen. Das männliche Reproduktionsverhalten sowie der Effect einer pulsatilen Verabreichung von GnRH mittels einer Hormonpumpe wird dargelegt.     

种公牛血清和精清睾酮与精液品质相关关系的研究

选取12头黑白花公牛,采用~(125)I标记的睾酮放射免疫测定法,对公牛血清和精清睾酮含量以及与精液品质的关系进行了研究。结果表明,血清和精清睾酮含量在个体间均存在着显著差异,睾酮含量与精液品质呈正相关。     

Effect of 131I-induced hypothyroidism on indices of reproductive function in adult male dogs

Hypothyroidism has been cited as a cause of infertility, abnormal semen quality, and poor libido in people and animals. The purpose of this study was to evaluate the effect of hypothyroidism on variables indicative of reproductive function in adult male dogs. Nine normal dogs were randomly assigned to 2 groups. Hypothyroidism was induced with 131I in 6 dogs. Three dogs remained untreated, normal, and euthyroid. Thyroid hormone concentrations, body weight, clinical signs, and reproductive function were determined for each dog every 3 months for 2 years. Reproductive function was assessed by determining daily sperm output, total scrotal width, spermatozoal motility and morphology, libido, and serum testosterone and luteinizing hormone concentration responses to exogenous gonadotropin-releasing hormone. The 131I-treated dogs developed clinical and laboratory signs of hypothyroidism. In the hypothyroid dogs, serum concentrations of thyroid hormones were consistently below the reference range and were significantly lower than that in the euthyroid dogs. There was no difference in reproductive function between the hypothyroid and euthyroid dogs. The results of this study show that 131I-induced hypothyroidism does not affect indices of reproductive function in adult male dogs.     

Review of present collection and research on forestry contracts of Qing Dynasty in Jinping County,Guizhou Province

Forestry contracts of the Qing Dynasty in Jinping County, southwest China‘s Guizhou Province, are a unique historical legacy of China. It came into being with the development of society and economy of Jinping. Research on these contracts is becoming widespread domestically and abroad as well. This paper is intended to make a review of the collection and research of forestry contracts in Jinping according to the chronological sequence.     

“三农”丛书出版要注意细节问题

论述了目前"三农"丛书出版要注意的细节问题,即丛书体例的统一,量和单位的规范统一,名词术语的使用,附件的核对等。     

中国粳稻核心种质耐低磷性的鉴定与筛选

鉴定粳稻耐低磷种质资源对我国粳稻耐低磷育种和耐低磷遗传研究具有重要意义.在田间低磷和正常施磷条件下,以单株相对籽粒产量为指标,对来自中国东北、华北、西北、华中、西南稻区的356份粳稻核心种质进行了耐低磷性鉴定.结果表明:东北稻区的平均相对籽粒产量最高,达0.81;华北稻区的相对籽粒产量最低.筛选出76个耐低磷材料、44个低磷敏感材料和236个中间类型材料;东北稻区耐低磷材料所占比例最高,西北稻区最低.     

The Spanish barley core collection

Spanish barleys constitute a germplasm group of particular interest for breeding purposes, as Spain has been proposed as a possible centre of origin of the crop. The Spanish National Germplasm Bank (Banco Nacional de Germoplasma, BNG), holds a collection of about 2000 barley accessions, mostly landraces collected in Spain prior to extensive introduction of modern varieties. The objective of this work is to create a core collection of barleys representative of old barley genotypes grown in Spain. The core collection will be constituted by three groups of germplasm: successful old varieties (15); entries in common with previously existing barley core collections (15); and 2-row (8) and 6-row (122) entries from the BNG, for a total of 160 entries. Entries were allocated by stratified sampling in agro-ecological uniform zones of barley cultivation in Spain. Classification of agro-ecological regions for barley was based on historical yield records for Spanish provinces. The number of entries for each region was determined in proportion to the logarithm of historical barley acreage. Final choice of accessions within provinces tried to maximize the diversity and avoid duplications by looking at passport data, and to agronomic evaluation data available for a group of about 900 accessions.     

Assessment of methods for the determination of digestibilities of feed ingredients for Tra catfish,Pangasinodon hypothalamus

In this study, we undertook a series of experiments to assess different faecal‐collection methods and also the digestibilities of a range of feed ingredients when fed to Tra catfish (Pangasinodon hypothalamus). Apparent digestibility coefficients for dry matter, energy and nitrogen for a reference diet were also determined from faeces collected by way of settlement at 2‐h intervals from 2 to 24 h. No significant effect of sample collection time on the digestibility values was observed. Stripping was attempted, but was not considered a suitable method for collecting digesta from Tra catfish. Dissection collection was also evaluated. Values determined from dissection collection were lower than those from using settlement. In a second experiment, a suite of test ingredients was combined with a reference diet component in a 30 : 70 ratio to determine the ingredients for dry matter, energy and nitrogen digestibilities. Ingredients included were trash‐fish, defatted rice bran, wet full‐fat rice bran, dried full‐fat rice bran, broken rice, cassava, soybean and fish meals. Collection of faeces by settlement was used for determining the digestibility of each of the ingredients. The results showed that Tra catfish can efficiently digest protein and energy from a wide range of feed ingredients and nutrient sources.     

牛精液冷冻技术应用研究进展

在简要介绍牛精液冷冻技术发展历史的基础上,分析了牛冷冻精液的现状和影响牛冷冻精液受胎率的主要因素,指出了我国牛冷冻精液存在的问题,应加强精液冷冻机理、精液冷冻保护剂、精子质量评估和分离精子的冷冻保存等方面的研究,最后展望了牛冷冻精液的发展前景。