INTRAVERTEBRAL DISC HERNIATION (SCHMORL'S NODE) IN FIVE DOGS

The herniation of intervertebral disc material into the vertebral body, known as Schmorl's nodes, is a well described disease process in man. Schmorl's nodes have not until now been described in the dog. This paper describes intravertebral disc herniation in the dog based on five patients taken from a retrospective study of dogs suspected of having cauda equina syndrome. The significance of intravertebral disc herniation in the dog at this time is that they indicate a disturbance in the vertebral endplate, they present definite radiographic signs, should be considered in patients with ostecohondrosis, and remain a possibls etiology for fibrocartilaginous emboli. Back pain is a common sign in people when Schmorl's nodes were the only pathologic findings. The common signs of palpatory pain in the lumbosacral region of the dogs in this report is noted but cannot be directly correlated at this time to the intravertebral disc herniation; however, it is suspected in two of the cases.     

长白猪肝脏、肾脏和淋巴结的显微形态及糖原PAS反应研究

[目的]对商品长白猪的组织形态学进行研究,了解商品长白猪宰前的生理状态。[方法]采集长白猪的肝脏、肾脏和淋巴结3种组织器官并制成石蜡切片,采用H.E染色方法对其组织结构进行研究。[结果]肾脏和肝脏的组织结构形态正常,肾脏和肝脏的细胞核染色明显,淋巴结的皮质和髓质分界不清晰,周围组织没有明显的淋巴索,淋巴窦较小。糖原PAS染色结果表明肝脏中的糖原颗粒较少,分布不均匀,而肾脏中糖原或糖蛋白多集中于血管球和肾小囊壁上。[结论]该研究可为今后进一步的商品长白猪理论研究奠定基础。     

定植和打顶及品种对雾培马铃薯器官建成的影响

该试验采用L9(34)正交试验设计,研究了不同定植节数、打顶次数及品种对雾培马铃薯植株茎粗、根系、LAI,LOX活性及结薯能力的影响.结果表明:雾培马铃薯定植3节及打顶2次处理均能促进LOX活性增强、匍匐茎发生,增加块茎数量和质量;结薯能力与品种差异相关密切,品种费乌瑞它结薯能力最佳;同时发现LOX活性增强利于雾培马铃薯结薯.品种费乌瑞它结合定植3节和打顶2次为雾培结薯最优组合.     

酷暑对兔体温和猪瘟定型热比率的影响

选用无猪瘟抗体原料兔作试验,研究酷暑对兔体温变化及注射猪瘟兔化弱毒脾淋乳浆液后产生定型热比率的影响。结果表明,当兔体周围气温达38℃时,100%的兔体温超过41℃;当环境温度达35.5℃时,100%的兔体温超过40℃。这些试验兔群表现出不同程度的中暑症状。发生中暑的试验兔群,在22℃的GMP生产动物房内饲养3 d后,每只注射含4 000个发热单位的猪瘟兔化弱毒脾淋乳浆液,5个试验组所产生的定型热比率分别为47%、65%、73%、54%、63%;而饲养于17~25℃舒适气温中的无猪瘟抗体兔群,转移到22℃的GMP生产动物房,注射相同病毒量后,产生定型热比率为98%、96%。上述结果表明在猪瘟兔化弱毒脾淋疫苗生产中,GMP生产动物房必须给兔子提供最舒适的室温,原料兔在生产后期和运输过程中也应避免中暑。但中暑过的试验兔,若能产生定型热,其脾淋毒价仍符合制造活疫苗的要求。     

管网方程Jacobi矩阵的一维压缩存贮与管网图节点标号的优化

利用大型稀疏矩阵的处理技术,用一维数组压缩存贮管网方程F(H)=0的Jacobi矩阵J(H),并应用图论方法进行管网图节点标号的优化,以节省管网分析计算所需的计算机存贮量及计算时间.     

转hrpZpsta抗病基因大豆的研究

【目的】将具有广谱抗病性的hrpZpsta基因导入大豆,为培育抗灰斑病的转基因大豆新品系奠定基础。【方法】采用农杆菌介导法,以大豆子叶节为受体,将具有广谱抗性的hrpZpsta基因转入大豆品种"吉林30"中,以耐盐基因badh作为筛选标记性基因,经过抗性筛选,对转基因植株进行PCR检测、Southern杂交和RT-PCR检测分析。【结果】确定的NaCl筛选浓度为200mmol/L。对T1、T2和T3代转基因植株进行PCR检测,得到T1代阳性植株30株,T2代45株,T3代284株,说明外源hrpZpsta基因在转基因后代中能够遗传。Southern杂交结果表明,外源目的基因hrpZpsta已经整合进大豆基因组中,且整合位点不尽相同。RT-PCR结果表明,hrpZpsta基因在受体大豆中获得表达。【结论】获得了hrpZpsta基因遗传表达的T3代转基因大豆株系。     

Toll-like receptor signaling is changed in ovine lymph node during early pregnancy

Toll-like receptors (TLRs) participate in regulation of adaptive immune responses, and lymph nodes play key roles in the initiation of immune responses. There is a tolerance to the allogenic fetus during pregnancy, but it is unclear that expression of TLR signaling is in ovine lymph node during early pregnancy. In this study, lymph nodes were sampled from day 16 of nonpregnant ewes and days 13, 16, and 25 of pregnant ewes, and the expressions of TLR family (TLR2, TLR3, TLR4, TLR5 and TLR9), adaptor proteins, including myeloid differentiation primary-response protein 88 (MyD88), tumor necrosis factor receptor associated factor 6 (TRAF6), and interleukin-1-receptor-associated kinase 1 (IRAK1), were analyzed through real-time quantitative polymerase chain reaction, Western blot, and immunohistochemistry analysis. The results showed that mRNA and protein levels of TLR2, TLR3, TLR4, TRAF6, and MyD88 were upregulated in the maternal lymph node, but TLR5, TLR9, and IRAK1 were downregulated during early pregnancy. In addition, MyD88 protein was located in the subcapsular sinus and lymph sinuses. Therefore, it is suggested that early pregnancy induces changes in TLR signaling in maternal lymph node, which may be involved in regulation of maternal immune responses in sheep.     

不同灌水条件下地下渗灌试验研究

地下渗灌是一种理想滴灌方式,由于关键技术没有很好解决,至今发展面积很小。针对这一问题,提出本试验研究。该试验是在实验室内的一个50cm×30cm×60cm的有机玻璃槽中进行,所用的土都是黏壤土。试验采用地下节点渗灌的方法。对试验所得的数据进行分析,得出地下渗灌的湿润锋运移基本规律、土壤含水率的分布规律,并在不同灌水量条件下对湿润锋的影响等进行了初步探讨。     

几种造纸用丛生竹秆节育苗试验

在福建省邵武市对椽竹、孝顺竹、坭竹等几种造纸用丛生竹进行秆节育苗试验表明，用平埋方法育苗显著优于斜埋和直插，竹蔸平埋效果更佳；不同竹种秆节育苗成活率存在明显差异；不同年龄的秆节育苗成活率在不同竹种间表现不同；不同育苗时间（３月或５月）的成活率差异不显著。竹秆不同粗细、不同部位、单节段或双节段育苗对比试验，结果以细秆（胸径２．５ｃｍ）、上部节段、双节段育苗成活率较粗秆（胸径４．６ｃｍ）、下部节段、单节段的为高。椽竹秆节径用不同激素处理后，处理间成苗率差异显著，以２，４－Ｄ２０ｍｇ／ｋｇ处理５ｈ和硼酸２０ｍｇ／ｋｇ处理２４ｈ的效果最佳。几种造纸用丛生竹秆节育苗试验＊张文燕周道三马乃训叶长青曹德友张华明     

抗生素对根癌农杆菌抑制作用及对大豆子叶节再生的影响分析

以周豆12大豆子叶节为材料,研究了头孢呋辛钠、头孢曲松钠、头孢噻肟钠和头孢唑林钠4种抑菌抗生素对农杆菌的抑制作用和对大豆子叶节再生的影响,以及卡那霉素对大豆子叶节再生的影响。结果表明:随着卡那霉素浓度的增加,子叶节分化率逐渐降低,当浓度达到45 mg/L时,分化芽的生长几乎完全受到抑制,植株出现白化现象;4种抑菌抗生素中,头孢曲松钠对农杆菌的抑制作用最好,头孢呋辛钠和噻肟钠抑菌效果次之,结合抑菌抗生素对子叶节再生的影响作用,确定在大豆子叶节的遗传转化中,使用头孢曲松钠或头孢噻肟钠作为抑菌抗生素,临界浓度分别为200 mg/L和300 mg/L。而卡那霉素使用的临界浓度为45 mg/L。