全文获取类型
收费全文 | 275篇 |
免费 | 6篇 |
国内免费 | 15篇 |
专业分类
林业 | 6篇 |
农学 | 12篇 |
8篇 | |
综合类 | 59篇 |
农作物 | 16篇 |
水产渔业 | 8篇 |
畜牧兽医 | 118篇 |
园艺 | 26篇 |
植物保护 | 43篇 |
出版年
2023年 | 1篇 |
2022年 | 4篇 |
2021年 | 3篇 |
2020年 | 7篇 |
2019年 | 1篇 |
2018年 | 2篇 |
2017年 | 4篇 |
2016年 | 6篇 |
2015年 | 4篇 |
2014年 | 6篇 |
2013年 | 12篇 |
2012年 | 30篇 |
2011年 | 24篇 |
2010年 | 21篇 |
2009年 | 23篇 |
2008年 | 24篇 |
2007年 | 30篇 |
2006年 | 15篇 |
2005年 | 9篇 |
2004年 | 8篇 |
2003年 | 8篇 |
2002年 | 9篇 |
2001年 | 9篇 |
2000年 | 5篇 |
1999年 | 4篇 |
1998年 | 5篇 |
1997年 | 2篇 |
1996年 | 4篇 |
1995年 | 6篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1986年 | 1篇 |
排序方式: 共有296条查询结果,搜索用时 15 毫秒
21.
AIM: To investigate whether gold nanoparticles (GNPs) reverses adriamycin (ADM), resistance of human hepatocellular carcinoma drug-resistant cell line HepG2/ADM and to explore the potential mechanism. METHODS: The sensitivities of HepG2 cells and HepG2/ADM cells to ADM were tested by MTT assay before and after GNPs pretreatment. The apoptotic rate was examined by flow cytometry. The concentration of ADM in HepG2/ADM or HepG2 cells was determined by ultraviolet-visible spectrophotometer. The content of glutathione (GSH) in HepG2/ADM or HepG2 cells by DTNB method. RESULTS: The half maximal inhibitory concentrations (IC50) of ADM for HepG2/ADM cells were(29.46±1.73) mg/L and (15.18±0.85) mg/L before and after GNPs pretreatment,respectively. The IC50 of ADM for HepG2 cells was (9.16±2.03) mg/L before pretreatment. The apoptotic rate in GNPs+ADM group was higher than that in ADM group (P<0.05). The concentration of ADM in HepG2/ADM group was lower than that in HepG2 group (P<0.01). After GNPs pretreatment, the concentration of ADM in HepG2/ADM cells was higher than that before pretreatment. The content of GSH in HepG2/ADM group was higher than that in HepG2 group (P<0.01). After GNPs pretreatment, the content of GSH in the HepG2/ADM cells was lower than that before pretreatment. CONCLUSION: Gold nanoparticles can reverse ADM resistance of human hepatocellular carcinoma drug-resistant cell line HepG2/ADM, reduce the content of GSH and increase the concentration of ADM in HepG2/ADM cells. 相似文献
22.
The effects of schisandrin B (Sch B) and its analogs on solar irradiation-induced oxidative injury were examined in BJ human fibroblasts. Sch B and schisandrin C (Sch C) increased cellular reduced glutathione (GSH) level and protected against solar irradiation-induced oxidative injury. The photoprotection was paralleled by decreases in the elastases-type protease activity and matrix-metalloproteinases-1 expression in solar-irradiated fibroblasts. The cytochrome P-450-mediated metabolism of Sch B or Sch C caused ROS production. The results suggest that by virtue of its pro-oxidant action and the subsequent glutathione antioxidant response, Sch B or Sch C may offer the prospect of preventing skin photo-aging. 相似文献
23.
抗日本血吸虫单克隆抗体的建立及特性测定 总被引:3,自引:1,他引:3
用感染日本血吸虫尾蚴的小鼠脾脏,制备单克隆抗体(McAb),获得24株抗日本血吸虫单抗,选择12株进行Ig亚型测定,对血吸虫抗原敏感性和特异性的测定,组织定位,免疫印迹,体外ADCC对血吸虫童虫的杀伤效应和体内被动免疫转移试验等方面的研究。其中一株IgM单抗在Balb/c小鼠体内诱导了38.41%的减虫率;另一株IgG#-1单抗在昆明系小鼠体内诱导了32.23%的减虫率,并观察到有抑制宿主肝脏肉芽肿形成的现象。 相似文献
24.
为了观察核酸疫苗SjCA/pc和SjMfl/pc在小鼠体内的表达,取昆明鼠,于其后腿外侧肌注免疫SjCA/pc和SjMfl/pc,2d后剖杀动物.取注射部位肌肉作石蜡切片,免疫组化分析,荧光显微镜下镜检。结果表明,SjCA/pc和SjMfl/pc在小鼠注射部位肌肉可见黄绿色荧光,而对照组无此现象。可见,SjCA/pc和SjMfl/pc可在小鼠体内表达。 相似文献
25.
镉对白菜活性氧代谢及H2O2清除系统的影响 总被引:10,自引:1,他引:10
研究不同浓度镉对白菜(Brassica campestris L.ssp.chinensis)叶片活性氧产生及H2O2清除酶活性影响。结果表明,培养液中加镉增加了白菜叶片中超氧自由基(O2-)的产生速率和H2O2含量,过氧化产物丙二醛(MDA)含量随培养液中镉浓度和镉处理时间增加而增加。在加镉条件下,抗坏血酸过氧化物酶(APX)活性、脱氢抗坏血酸还原酶(DR)和谷胱甘肽还原酶(GR)活性升高,抗坏血酸(AsA)和谷胱甘肽(GSH)含量增加。 相似文献
26.
AIM: To investigate the potential mechanisms of renoprotective effect of grape seed proanthocyanidin (GSP) on diabetic nephropathy.METHODS: Male Wistar rats were injected with 1% streptozotocin (STZ) intravenously to induce diabetes mellitus (DM). The diabetic rats were randomly divided into 2 groups: diabetes group (DM group) and GSP treatment group (GSP group, GSP 250 mg·kg-1·d-1). The normal Wistar rats served as control (C group). Body weight (BW), systolic pressure, kidney weight/body weight (KW/BW), fasting plasma glucose (FPG), blood urea nitrogen (BUN), serum creatinine (SCr), glycosylated hemoglobin (HbA1c) and 24 h urine protein were determined 24 weeks after STZ intervention. The pathological changes of the renal tissues were observed. The protein levels of glutathione S-transferase mu (GSTM) and nuclear factor-erythroid 2-related factor 2 (Nrf2) in the renal tissues were determined by Western blotting and immunohistochemistry. RESULTS: Compared with C group, BW in diabetic rats decreased (P<0.01). The levels of systolic pressure, FPG, HbA1c, KW/BW, 24 h urine protein, BUN and SCr in DM group were higher than those in C group (P<0.01). After treated with GSP, the levels of systolic pressure, KW/BW, 24 h urine protein, BUN and SCr in DM rats were lower than those in DM rats without treatment (P<0.01 or P<0.05). The pathological changes were ameliorated in GSP group. The expression of GSTM and Nrf2 was up-regulated in the kidneys of diabetic rats and down-regulated to the normal levels after GSP treatment. CONCLUSION: The renoprotective effect of GSP is associated with the down-regulation of GSTM through modulating the expression of Nrf2. 相似文献
27.
Smita JainRafat S. Ahmed Vinod Kumar AroraBasu Dev Banerjee 《Pesticide biochemistry and physiology》2011,100(2):182-186
Triazophos, O,O-diethyl-1-H-1,2,4-triazol-3-yl phosphorothioate, (TZ) is an organophosphorus pesticide which is extensively used in agriculture for controlling insect pests. Except a FAO/WHO report no study has investigated its short-term toxicity with respect to its potential to cause biochemical and histopathological alterations. The present study was designed to identify the effect of TZ at different doses (1.64, 3.2 and 8.2 mg/kg) on the oxidative stress parameters in blood as well as organs involved in xenobiotic metabolism (liver and brain) following chronic exposure for 90 days. Moreover, the study also delineates the effect of TZ on the histo-architecture of these organs. The results indicated a dose dependent induction (p < 0.001) of oxidative stress, as evident by increased malondialdehyde (MDA) level and compromised antioxidant defense including glutathione S transferase (GST) activity, glutathione (GSH) content and ferric reducing ability of plasma (FRAP) in blood, and increased MDA level with concomitantly decreased GSH content in tissues, following chronic exposure to TZ. The ratio of MDA: FRAP in blood was found to be increased following chronic exposure to TZ and may serve as a suitable indicator of severity of oxidative damage. Onset of such biochemical alterations is one of the early adaptive responses to TZ exposure which leads to histopathological alterations in terms of diffuse fatty changes expanding from mid-zonal area to whole lobule in liver. However, increased oxidative stress did not bring any morphological alteration in brain. The present study concludes that induction of oxidative stress, leading to subsequent histopathological alterations in liver, is an important mechanism underlying the TZ induced chronic toxicity. 相似文献
28.
本文报告了日本血吸虫童虫经液氮冻藏复苏后的生活史循环的情况。童虫冻藏36天和50天后腹腔注射感染小鼠,亲代成虫回收率为9.0和8.9%。感染鼠肝内的子代虫卵孵出的子1代毛蚴感染钉螺,感染率为59和67%;平均每个钉螺第1次逸出的子1代尾蚴数为800~900条。子1代尾蚴感染小鼠后,其子1代成虫回收率为55和56%。合抱雌、雄成虫的体长、性腺的发育和虫体对宿主的致病力与正常途径感染者无明显差异。 相似文献
29.
30.
The tarnished plant bug (TPB) has increasingly become an economically important pest of cotton. Heavy dependence on insecticides, particularly organophosphates and pyrethroids, for TPB control facilitated resistance development to multiple classes of insecticides. To better understand resistance and explore ways to monitor resistance in field populations, this study examined acephate susceptibility and the activities of two major detoxification enzymes in nine field populations collected in the Delta region of Mississippi and Arkansas in 2010. Two Arkansas populations from Reed and Backgate had 3.5- and 4.3-fold resistance to acephate, as compared to a susceptible laboratory strain. Extensive planting of cotton and heavy chemical sprays is a major driving force for resistance development to acephate in Mid-south cotton growing areas. Reduced susceptibility to acephate was highly correlated with elevated esterase activities. The acephate-resistant populations from Backgate, Lula, and Reed consistently had higher (up to 5.3-fold) esterase activities than susceptible populations. Regression analysis of LC50s with kinetic esterase activities revealed a significant polynomial quadratic relationship with R2 up to 0.89. Glutathione S-transferase (GST) also had elevated activity in most populations, but the variations of GST activities were not significantly correlated with changes of acephate susceptibility. Finally, examination of esterase and GST inhibitors indicated that suppression rates (up to 70%) by two esterase inhibitors in 2010 were slightly lower than those detected in 2006, and ethacrynic acid (EA) inhibited GST effectively in both years. Two other GST inhibitors (sulfobromophthalein and diethyl maleate) displayed significantly lower suppression rates in 2010 than those detected in 2006, suggesting a potential genetic shift in pest populations and a necessity of continued monitoring for insecticide resistance with both bioassay and biochemical approaches. Results indicated that using major detoxification enzyme activities for resistance monitoring may provide insight into acephate resistance in field populations of TPB. 相似文献