硝基咪唑类药物检测技术研究进展

本文介绍了硝基咪唑类药物的理化性质、作用、应用现状及检测方法,重点论述了动物组织,产品及排泄物中药物残留和饲料中原药的检测技术。     

牛磺酸的生物学功能及其在蛋鸡生产中的研究应用

牛磺酸是一种具有广泛生物学功能的营养物质,它在畜牧业生产中的应用已引起人们的密切关注,成为当今研究的一个亮点。本文概述了牛磺酸的结构、性质、分布、合成代谢、生物学功能及其在蛋鸡生产中的研究应用进展,并指出了今后需解决的问题和发展前景。     

AR regulates porcine immature Sertoli cell growth via binding to RNF4 and miR-124a

Sertoli cells are the only somatic cells in the seminiferous epithelium which directly contact with germ cells. Sertoli cells exhibit polarized alignment at the basal membrane of seminiferous tubules to maintain the microenvironment for growth and development of germ cells, and therefore play a crucial role in spermatogenesis. Androgens exert their action through androgen receptor (AR) and AR signalling in the testis is essential for maintenance of spermatogonial numbers, blood–testis barrier integrity, completion of meiosis, adhesion of spermatids and spermiation. In the present study, we demonstrated that AR gene could promote the proliferation of immature porcine Sertoli cells (ST cells) and the cell cycle procession, and accelerate the transition from G1 phase into S phase in ST cells. Meanwhile, miR-124a could affect the proliferation and cell cycle procession of ST cells by targeting 3′-UTR of AR gene. Furthermore, AR bound to the RNF4 via AR DNA-binding domain (DBD) and we verified that RNF4 was necessary for AR to regulate the growth of ST cells. Above all, this study suggests that AR regulates ST cell growth via binding to RNF4 and miR-124a, which may help us to further understand the function of AR in spermatogenesis.     

牛星状病毒EvaGreen实时荧光定量PCR检测方法的建立及应用

牛星状病毒(BAstV)是我国新发的犊牛腹泻病原,本试验的目的是建立检测BAstV的Real-time PCR方法.根据BAstV流行株的ORFla基因序列设计引物,通过优化反应条件和体系,成功建立基于EvaGreen检测BAstV的Real-time PCR方法.结果表明,该检测方法的Ct值与标准品模板在1.36×1...     

Trade-off expression of melatonin receptor subtypes (Mel1a and Mel1b) and androgen receptor in lung of a tropical bird,Perdicula asiatica

The role of circulatory steroid hormone along with melatonin in lung of any seasonally breeding bird has never been explored so far. This could be interesting because steroid hormones are immunosuppressive while melatonin is immunostimulatory in nature. In our present study, we report the effect of exogenous melatonin and testosterone on expression of melatonin receptor subtypes (Mel_1a and Mel_1b) and androgen receptor in lung of a tropical bird Perdicula asiatica. Birds were collected from vicinity of Varanasi and acclimatized in laboratory with sufficient food and water. The birds were treated with melatonin and testosterone at dose of 25 µg/100 g B.wt./day and 1 mg/100 g B.wt./day, respectively, for 28 days. At the end of the experiment, the birds were sacrificed and lung tissue and blood sample were collected for immunohistochemistry, Western blot analysis and hormonal assay. Testosterone treatment increased circulatory testosterone and upregulated expression of androgen receptors whereas downregulated expression of melatonin receptor subtypes Mel_1a and Mel_1b. Melatonin administration increased peripheral melatonin and upregulated expression of melatonin receptor subtypes Mel_1a and Mel_1b while downregulated androgen receptor. Thus, our results suggest that a trade-off relationship between melatonin and testosterone exists in regulation of their receptors in lung of Perdicula asiatica.     

甜菜碱对生长肥育猪胴体组成的影响及其机理研究进展

甜菜碱是一种广泛存在于动植物体内的天然化合物,无毒、无害、无残留、无污染,性质稳定。在动物体内,甜菜碱是高效的甲基供体物质,参与蛋白质和脂肪代谢。日粮中添加甜菜碱可提高猪的生长性能、改善胴体组成和肉质。本文就甜菜碱对生长肥育猪胴体组成的影响及其机理研究作一综述。     

鸡毒支原体强弱毒株荧光定量PCR鉴别检测方法的建立

为建立一种能鉴别鸡毒支原体(MG)强、弱毒株的快速检测方法,本研究根据GenBank中MG强毒株和弱毒株的基因组序列,选取特异性保守区序列设计了2对引物和2奈探针,分别用于强弱毒株和弱毒株的检测,优化反应条件,建立了能区分MG强、弱毒株的荧光定量PCR检测方法.该法特异性强,对鸡常见呼吸道病原体的反应均为阴性;灵敏度高,可检测到100拷贝/μL的模板;稳定性好,批内和批间试验Ct值的变异系数小.本研究建立的MG强、弱毒鉴别检测方法简便、快捷,为该病的防控与净化提供新方法、新思路.     

黄鳝的营养需求研究及其配合饲料质量评价

文章概述了黄鳝(Monopteras albus)的营养需求研究及其配合饲料质量评价的进展,主要包括黄鳝的蛋白质、脂肪、碳水化合物、维生素等营养需求研究及其配合饲料质量评价体系,并对今后开展黄鳝营养需求研究及构建完善的黄鳝配合饲料质量评价体系提出了若干建议。     

精氨酸营养生理功能研究新进展

精氨酸作为一种条件性必需氨基酸,不仅是合成蛋白质的重要原料,而且在生理调节方面具有重要的功能。近来年有关精氨酸营养生理功能的研究又取得了许多突破性的进展,本文对精氨酸在促进孕期及新生期胎儿生长、特异性减少脂肪含量、调控肿瘤细胞生长、改善心血管系统疾病、免疫调理等方面的最新研究成果以及过量添加精氨酸对动物机体的影响进行了综述,以促进精氨酸在相关领域的应用及其研究的深入。