Origin of Clostridium perfringens isolates determines the ability to induce necrotic enteritis in broilers

Since the ban on growth-promoting antibiotics in animal feed in the European Union, necrotic enteritis has become a major cause of mortality in broiler chickens. Despite the importance of the disease, the pathogenesis is still not completely understood. In the current study, Clostridium perfringens strains isolated from healthy flocks and isolates from outbreaks of necrotic enteritis were evaluated for the ability to cause gut necrosis in an intestinal loop model in laying hens and in an experimental infection model in broilers. High, intermediate and low alpha toxin producing strains were chosen from each isolation source. Only the isolates from field outbreaks induced necrotic gut lesions, independent of the amount of alpha toxin produced in vitro. It was also shown that alpha toxin producing isolates from calf hemorrhagic enteritis cases were not able to induce necrotic enteritis in poultry. These results suggest the presence of host specific virulence factors in C. perfringens strains, isolated from chickens with intestinal necrotic enteritis lesions.     

Effect of the Antisense BcMF12 Driven by the BcA9 Promoter on Gene Silencing in Brassica campestris L. ssp. chinensis

The study analyzed the silencing of BcMF12 gene regulated by BcA9 promoter in the transgenic pakchoi and confirmed the effect of antisense BcMF12 gene on the pollen development. A conserved BcMF12 gene fragment was amplified from the cDNA of flower buds in pakchoi (Brassica campestris L. ssp. chinensis, syn. B. rapa L. ssp. chinensis) and was fused to the anther specific BcA9 promoter. The plant antisense expression vector was constructed and then introduced into pakchoi via Agrobacterium-mediated transformation. The transgenic plants were screened by antibiotics and molecular analysis. PCR and Southern blot revealed that the antisense BcMF12-GUS fusion gene regulated by BcA9 promoter was integrated into transgenic plants. Northern blot suggested that the expression of BcMF12 gene was down-regulated significantly. The pollen germination rate of transgenic plants with antisense BcMF12 gene decreased as compared with that of the control plants. The expression of the gene BcMF12 related to the pollen development was inhibited by the antisense BcMF12 driven by BcA9 promoter, which consequently affected the pollen development in pakchoi.     

Conus regius-Derived Conotoxins: Novel Therapeutic Opportunities from a Marine Organism

Conus regius is a marine venomous mollusk of the Conus genus that captures its prey by injecting a rich cocktail of bioactive disulfide bond rich peptides called conotoxins. These peptides selectively target a broad range of ion channels, membrane receptors, transporters, and enzymes, making them valuable pharmacological tools and potential drug leads. C. regius-derived conotoxins are particularly attractive due to their marked potency and selectivity against specific nicotinic acetylcholine receptor subtypes, whose signalling is involved in pain, cognitive disorders, drug addiction, and cancer. However, the species-specific differences in sensitivity and the low stability and bioavailability of these conotoxins limit their clinical development as novel therapeutic agents for these disorders. Here, we give an overview of the main pharmacological features of the C. regius-derived conotoxins described so far, focusing on the molecular mechanisms underlying their potential therapeutic effects. Additionally, we describe adoptable chemical engineering solutions to improve their pharmacological properties for future potential clinical translation.     

H9N2亚型禽流感流行现状

低致病性禽流感主要是由H9N2亚型禽流感病毒所引起,近几年在世界上许多国家都暴发了H9N2亚型禽流感疫情.研究表明,H9N2亚型禽流感病毒在陆禽中至少可分为北美和欧亚两个种系,该病毒在自然环境中很容易发生变异,通过混合基因组分而形成不同的病毒亚型.H9N2亚型禽流感病毒已经能传播至哺乳动物,包括猪和人类,从而引发对其是...     

高油分优质油菜盛湘油9号在长江中游种植表现及栽培技术

[目的]为了加快盛湘油9号在长江中游的推广应用.[方法]重点介绍盛湘油9号在长江中游区的种植表现及主要性状特点和栽培技术.[结果]盛湘油9号在国家中游区油菜区域试验和生产试验中,表现出具有高油、丰产特点.两年区试平均产量和产油量分别达2 347.8和1 080.45 kg/hm^2,平均比对照（中油杂2号）分别减产0.82％和增产8.25％.两年平均含油率为46.02％、芥酸含量0.25％、硫苷含量18.45 μmol/（g·饼）.生产试验中平均产量达2 119.5 kg/hm2,平均比CK中油杂2号增产0.73％,于2009年12月通过国家品种审定委员会审定.[结论]该研究可用以指导油菜大面积生产,提高产量.     

Effects of the Polish Rootstocks P2 and P22 on Vigour,Yield and Fruit Characteristics of the Apple Cultivar ‘Gravenstein’

Abstract

The performance of the Polish rootstocks P2 and P22 under Norwegian growing conditions was evaluated over 10 years (1983–92). Tree vigour of the apple cultivar ‘Gravenstein’ on P2 and P22 was 64 and 16% of that on M9, respectively. Trees on P2 had the highest yield efficiency, but less total yield per tree than M9. Owing to very dwarfing growth, trees on P22 failed to establish a sufficient canopy and produced significantly smaller apples. P2 and P22 affected apple soluble solids in a favourable way, but did not influence fruit acidity.     

少刺型黄瓜新品种中农9号的选育

中农 9号黄瓜是用自交系 183和 185配制而成的少刺、短瓜型高产多抗一代杂种。中早熟 ,生长势强 ,耐低温弱光性好。抗黑星病、枯萎病、细菌性角斑病 ,中抗霜霉病等。每 6 6 7m2 产量 75 0 0～ 15 0 0 0kg。适宜日光温室、春棚及秋棚延后栽培 ,以日光温室越冬嫁接栽培效果最好。已在山东莱西、莱阳、青岛、寿光及江苏等地推广 10 0 0hm2 以上。     

牛GDF-9基因的克隆与组织表达的分析

为了检测牛GDF-9基因mRNA在怀孕期牛生殖系统的表达情况,为GDF-9基因在雌性动物繁殖上的研究提供一定的理论依据。本试验根据GenBank中报道的牛GDF-9基因序列设计引物,从牛卵巢、输卵管、子宫、肾脏中提取RNA,采用RT-PCR技术进行cDNA扩增,扩增产物与载体pGM-T连接,转化大肠杆菌DH5α,筛选阳性克隆,测序。结果表明,已成功克隆的阳性重组子经测序鉴定其片段大小与预期大小完全一致,为264 bp。序列同源性分析比较表明,该cDNA及其推导的氨基酸序列与绵羊GDF-9cDNA和氨基酸序列同源性分别为97%和94%。以-βactin为内参照物,采用RT-PCR技术进行半定量分析,通过检测比较牛GDF-9基因在卵巢、输卵管、子宫、肾脏组织中mRNA的表达情况。结果表明,牛GDF-9基因在卵巢中表达量最高,在输卵管、子宫、肾脏中均有表达,但表达量不高。     

重庆地区叶用枸杞“宁杞9号”扦插育苗技术研究

为了探究适宜重庆地区的叶用枸杞“宁杞9号”扦插育苗技术,进行了不同扦插基地配比处理下的叶用枸杞扦插育苗技术研究。结果表明:河沙∶椰棕土∶草炭土∶有机肥(3∶3∶3∶1)的基质配比扦插效果最好,“宁杞9号”扦插成活率达到90.25%,扦插苗生根数量4.05根,扦插苗新生根长4.26 cm,扦插苗株高28.39 cm,扦插苗新生枝条粗度0.43,而新生枝条数量无显著差异。