The rates of maize growth and lignin biosynthesis change after root-applied chalcone

The effects of root-applied chalcone at 0.15 mmol L⁻¹ on the growth and lignin biosynthesis in maize were investigated. The contents of 4-coumarate: CoA ligase (4CL, EC 6.2.1.12) substrates in maize shoots were increased more rapidly in the samples with chalcone application than in the control and the increase occurred at ≤ 3 h after the application (HAA). The lignin content was reduced by chalcone at ≤ 6 HAA. The shoot growth was suppressed by chalcone at ≤ 9 HAA. Consequently, the results suggest that chalcone suppressed maize growth by inhibiting monolignol biosynthesis.     

保护地番茄新品种苏粉8号的选育

苏粉8号系以GB9736为母本,TM9761为父本配制而成的适合保护地栽培的中熟番茄一代杂种.属无限生长型.果实高圆形,粉红色,果面光滑,果皮厚,耐贮运,品质佳,可溶性固形物含量5.0%,酸甜适中,单果质量200～250 g,产量6 000kg·(667 m2)-1以上.高抗ToMV-0,1株系、叶霉病,抗枯萎病,中抗CMV.已在江苏、山东、四川、浙江等地示范推广逾266.7 hm2.     

黄瓜新品种津优12号的选育

用优良自交系Q12作母本,F51作父本配制的一代杂种津优12号,植株生长势中等,叶色深绿,瓜条顺直,长棒状,长35 cm左右;单瓜质量200g左右,瓜色深绿,有光泽,瘤显著,密生白刺,果肉淡绿色,质脆,味甜,畸形瓜率低;春季主蔓第1雌花着生在第4节左右,雌花节率高.春露地栽培产量可达5 500kg·(667m2)-1左右,秋大棚栽培产量4 200kg·(667 m2)-1.抗枯萎病、霜霉病、白粉病和CMV.适宜华北、东北、西北地区春露地和秋大棚栽培.     

夏秋萝卜新品种丰玉一代的选育

丰玉一代是以雄性不育系4-04A为母本，S30—1为父本配制的一代杂种，属夏秋萝卜类型，生育期70～75d（天），肉质根圆柱形，长25—30cm，粗8—10cm，皮色全白，表皮光滑，含水量适中，肉质细密脆嫩，单根质量1，0—1．5kg。每667m^2产量2500kg左右，适宜山西省夏秋季种植。     

大蒜新品种成蒜早2号和成蒜早3号的选育

成蒜早2号和成蒜早3号是从温江蒜的自然变异株中,经系统选育而成的早熟新品种.成蒜早2号从播种到蒜薹始收需153～180 d(天),平均单薹质量12.7 g,单头质量10.7 g,多数蒜头由6～9瓣组成,蒜瓣紧密抱握蒜薹轴,一般蒜薹和蒜头产量分别为340和350 kg·(667 m2)-1,抽薹比温江蒜早,抗寒力比温江蒜弱;成蒜早3号从播种到蒜薹始收需153～186 d(天),平均单薹质量和单头质量分别为14.5和12.0 g,多数蒜头由5～8瓣组成.一般蒜薹和蒜头产量分别为320和410kg·(667 m2)-1,抽薹比温江蒜早,抗寒力与温江蒜相当.     

日光温室黄瓜济杂3号的选育

母本88001来源于津杂2号,父本88010来源于津研2号与新泰密刺的杂交后代。其一代杂种济杂3号,瓜条顺直,长30～35 cm,单瓜质量约180 g,瓜把短,瓜皮深绿,密瘤白刺,品质优,商品性好,对霜霉病、白粉病、枯萎病抗性强。每667m~2产量7000～14000 kg,适合日光温室越冬茬和早春茬栽培。     

Detection,cloning and expression of bone morphogenetic protein-1 from human osteosarcoma cell lines

AIM:To manufacture recombinant protein of the highly conserved domain in human bone morphogenetic protein-1(BMP-1) using gene engineering methods as antigen for making wide spectrum antibody to BMP-1.METHODS:We analyzed the gene sequences and protein structures of BMP-1 and its related proteins, and chose a highly conserved fragment as target gene. Total RNA was prepared from human osteosarcoma cell line Saos-2, then the target gene was amplified with RT-PCR. The PCR product was cloned into prokaryotic expression vector pMAL c2 to get recombinant vector BMP-1(322-588aa)-pMAL c2. After transforming the recombinant plasmid into DH5-alpha and screening, several prositive clones were got for sequencing. Finally the transformed cells was induced with IPTG to get fusion protein.RESULTS:The BMP-1 gene fragment was successfully cloned into vector pMAL c2, and was able to express efficiently with IPTG inducement. The amount of expressed fusion protein is about 66%-72% in total volume of bacterial proteins.CONCLUSIONS:The recombinant protein contains several key domains(2 CUB domains and 1 EGF domain), which are shared by BMP-1 and its related proteins. Specific wide spectrum antibody to human BMP-1 and its related proteins may be generated with this recombinant protein antigen.     

少刺型黄瓜新品种中农9号的选育

中农 9号黄瓜是用自交系 183和 185配制而成的少刺、短瓜型高产多抗一代杂种。中早熟 ,生长势强 ,耐低温弱光性好。抗黑星病、枯萎病、细菌性角斑病 ,中抗霜霉病等。每 6 6 7m2 产量 75 0 0～ 15 0 0 0kg。适宜日光温室、春棚及秋棚延后栽培 ,以日光温室越冬嫁接栽培效果最好。已在山东莱西、莱阳、青岛、寿光及江苏等地推广 10 0 0hm2 以上。     

茄子新品种蒙茄4号的选育

蒙茄 4号是以 86D0 1 1 1 1为母本 ,79B0 1 2 2 2为父本配制的一代杂种。该品种中早熟 ,始花节位第 7～ 8节。果实近圆形稍扁 ,果皮深紫色 ,光泽度强 ,耐贮运 ,果肉紧密 ,商品性好。平均单果质量 4 0 0～ 4 5 0g,露地每6 6 7m2 产量 4 5 0 0kg左右。适宜露地栽培。     

优质茄子新品种引茄1号的选育

引茄 1号为杂种一代 ,植株生长势旺盛 ,果实细长、紫红色 ,果面光滑 ,果肉细嫩 ,平均单果质量 6 0～70kg ,抗青枯病和绵疫病 ,产量 30 0 0～ 4 0 0 0kg·(6 6 7m2 ) -1,适于我国喜食长茄地区的保护地和露地栽培。