Effects of dietary inclusion of Rhus coriaria on internal milieu of rabbits

The aim of the present work was to investigate the effect of Rhus coriaria L. inclusion to the diet on some biochemical, haematological parameters and the level of antioxidant status of male rabbits. Adult rabbits were divided into five groups: one control (C) and four experimental groups. Experimental animals received sumac per os in feed in various doses (0.50%, 0.75%, 1.00% and 1.50%) for 90 days. Significant increase in PDWc (platelet distribution width) in E3 group when compared with control group was recorded. Sumac administration resulted in decreased cholesterol levels in all experimental groups vs. control group. Significantly lower level of cholesterol was found in E4 group with highest dose of sumac (1.50%). Higher values of total antioxidant status (TAS) and albumins were observed in all experimental groups in comparison with control group. A significant increase in TAS was detected in group E1 and E4. Concentrations of albumins were significantly higher in groups E3 and E4 vs. control group. Sumac administration had no significant effect on bilirubin content. In conclusion, these results show a positive effect of sumac consumption on antioxidant status and cholesterol level in adult male rabbits.     

温度和季节对四川白獭兔部分繁殖性状的影响分析

环境因素特别是温度、光照对家兔的繁殖力影响很大。为评估亚热带地区温度和季节对四川白獭兔繁殖性状的影响,对四川成都邛崃市某兔场2008-2009年的四川白獭兔种兔部分繁殖资料进行了相关性分析。结果表明:温度和季节对四川白獭兔的窝平均产仔数、初生仔兔死亡率的影响明显,夏季高温条件下四川白獭兔的窝平均产仔数最低为6.00只;而冬春季节较高,分别为6.19只和6.16只。夏季初生兔死亡率最高为0.07%,而秋季最低为0.03%。     

低蛋白质饲粮中添加尿素对肉兔消化代谢的影响

本试验旨在研究低蛋白质饲粮中添加尿素对肉兔消化代谢的影响。采用单因子随机区组试验设计,选择 ９０只 ５０日龄体重相近的健康新西兰兔,随机分为 ３组,１个对照组和 ２个试验组,每组 １５个重复,每个重复 ２只。对照组饲喂粗蛋白质含量为 １１．１１％的基础饲粮,２个试验组分别饲喂在基础饲粮中以糊化淀粉缓释尿素的方式添加 １％、２％和 ３％尿素的试验饲粮。预试期为 １０ｄ,正试期为 ３０ｄ。正试期的第 １３～１８天收集试验兔排出的全部粪便和尿液。结果表明：与对照组相比,１％尿素组试验兔的有机物表观消化率、总能表观消化率及利用率、沉积氮、氮利用率和消化氮利用率显著或极显著升高（Ｐ＜０．０５或 Ｐ＜０．０１）,而 ２％尿素组试验兔的消化能、代谢能、沉积氮、氮利用率和消化氮利用率显著或极显著降低（Ｐ＜０．０５或 Ｐ＜０．０１）。对照组试验兔盲肠内容物脲酶活性和微生物蛋白浓度显著或极显著低于其余 ２组（Ｐ＜０．０５或 Ｐ＜０．０１）,而 ２％尿素组试验兔盲肠内容物氨氮和尿素氮浓度显著或极显著高于其余 ２组（Ｐ＜０．０５或 Ｐ＜０．０１）。由此得出,低蛋白质饲粮中添加 １％尿素可改善肉兔有机物、总能和氮的利用效率,提高盲肠内容物脲酶活性和微生物蛋白浓度;添加 ２％尿素则会降低肉兔氮的利用效率,提高盲肠内容物脲酶活性及微生物蛋白、氨氮和尿素氮浓度。     

鸡蛋提取物角蛋白及鸡蛋黄对兔伤口愈合的临床研究

为了探讨鸡蛋提取物角蛋白及鸡蛋黄对伤口愈合影响的临床研究,取健康家兔12只,随机分为A、B两组,每组6只,在每只家兔脊背两侧设试验伤口和对照伤口各1个,即同体不同部位对照试验;A组试验处伤口涂抹鸡蛋提取物角蛋白;B组试验处伤口涂抹生鸡蛋黄;A、B两组对照处伤口均未作任何处理。结果表明:A组和B组涂抹鸡蛋提取物角蛋白和生鸡蛋黄的伤口愈合时间比未做任何处理的伤口愈合快3～4 d,无论是愈合速度还是愈合效果均非常明显且伤口感染率减少,伤口愈合的疤痕减淡,外部更加美观自然,且能达到祛疤美容效果。     

圆叶决明草粉替代不同比例精料喂兔效果研究

利用豆科牧草圆叶决明Chamaecrista routundifolia CPI34721草粉分别以10%,15%,20%不同比例替代精料进行肉兔喂养试验.试验结果表明:与对照相比较,在兔只平均日增重、饲料利用率方面,各处理与对照差异不显著.替代10%和15%精料处理可显著提高兔肉品质,替代20%精料的处理在经济效益方面最佳,可提高经济效益5.15%.     

Effects of conditioned media from human amniotic epithelial cells on corneal alkali injuries in rabbits

This study was performed to evaluate the effects of conditioned media (CM) from human amniotic epithelial cells (HAECs) on the corneal wound healing process. Eighteen rabbits (36 eyes) were used and randomly assigned to three groups according treatment: CM from HAECs (group 1), vehicle alone (group 2), and saline (group 3). Corneal alkali injuries were induced with 1 N sodium hydroxide. Each reagent used for treatment evaluation was injected into the dorsal bulbar subconjunctiva and the area of the corneal epithelial defect was measured every other day. Two animals from each group were euthanized at a time on days 3, 7, and 15, and the cornea was removed for histological examination. The sum of the epithelial defect areas measured on day 0 to day 6 as well as day 0 to day 14 in group 1 was significantly smaller than those of other groups. Histological examination revealed that the group 1 corneas had less inflammatory cell infiltration and showed more intact epithelial features compared to the other groups. These results suggest that CM from HAECs promote corneal wound healing in rabbits.     

发酵苹果渣对獭兔生长性能、养分消化率、小肠黏膜形态及分泌型免疫球蛋白A浓度的影响

本试验旨在研究发酵苹果渣对獭兔生长性能、养分消化率、小肠黏膜形态及分泌型免疫球蛋白A(SIgA)浓度的影响。试验共选用96只、45日龄的健康獭兔,随机分为4组,每组4个重复,每个重复6只(公母各占1/2)。4组獭兔分别饲喂含0(对照,CK组)、9.6%(A组)、19.2%(B组)和28.8%(C组)发酵苹果渣的试验饲粮。预试期15 d,正试期50 d。结果显示:与CK组相比,C组獭兔平均日增重提高了14.69%(P <0.05),料重比降低了6. 63%(P <0. 05),腹泻率降低了19.64%(P<0.05); C组獭兔饲粮干物质、有机物、粗蛋白质和粗纤维消化率分别提高了6.11%(P<0.05)、5.19%(P<0.05)、7.26%(P<0.05)和13.10%(P<0.05); C组獭兔空肠的绒毛长度和肠壁厚度分别增加了59.82%(P<0.05)和41.83%(P<0.05),同时十二指肠的隐窝深度降低了30.52%(P<0.05)。添加不同水平的发酵苹果渣对獭兔小肠中SIgA浓度无显著影响(P>0.05)。综上所述,在饲粮中添加28.8%的发酵苹果渣可以通过提高养分消化率,改善小肠黏膜形态来提高獭兔的生长性能,并可降低腹泻率,且对小肠中SIgA浓度无不良影响。     

Evaluation of the carryover effect of antibiotic,bee pollen and propolis on growth performance,carcass traits and splenic and hepatic histology of growing rabbits

Sixty‐four nulliparous female rabbits were distributed among eight groups (eight animals/group). Group one was the unsupplemented control group; the other seven groups were supplemented with zinc bacitracin (ZnB) at 100 mg, or bee pollen (BP) and/or propolis (Pro) at 150 and 300 mg in a capsulated form, three times a week, day after day, continuously all over the experimental period. The experiment was run for eight parties; at each parity, 28 kids of each doe group (a total of 224 rabbits) were divided into two subgroups weaned, respectively, at 24 and 30 days of age. Thus, for each parity, there were 16 groups (eight does treatments × two weaning age, 14 rabbits per group). The growing rabbits fed the standard diets without supplements. The growth performance, the carcass traits, the liver and the spleen histology of rabbits were checked up to 90 days of age to find possible carryover effects of the supplements. The supplements had no significant effect on most of the growth performance at 90 days of age, but BP150 and BP+Pro300 increased the growth rate in comparison with ZnB group. The liver weight in the control, BP300 and Pro300 groups was higher than the ZnB one. The spleen weight was higher in the groups ZnB, BP150, Pro300 and BP+Pro300, followed by the control, BP300 and BP+Pro150 and thus Pro150. The heart % in the BP150 and Pro300 groups was higher than ZnB and BP+Pro150 groups. A lymphoid hyperplasia of splenic white pulp was observed in the BP+Pro groups, while propolis alone showed a mild activation of lymphobiosis. The Pro and BP groups showed the same picture of the control group exhibiting a hydropic degeneration of mostly hepatic cells, while the ZnB group exhibited adverse effect on the bile ducts featuring portal periductal inflammatory cells infiltration with epithelial hyperplasia reflecting chronic cholangitis.     

Effect of the β‐carotene oxygenase 2 genotype on the content of carotenoids,retinol and α‐tocopherol in the liver,fat and milk of rabbit does,reproduction parameters and kitten growth

Mutations in the β‐carotene oxygenase 2 (BCO2) gene can impair the function of the enzyme that breaks down carotenoids. As a result, gradual accumulation of unoxidized carotenoids in animal tissues gives them a yellow colour. The aim of the study was to determine the content of carotenoids, retinol and α‐tocopherol in the liver, fat and milk of rabbit does with three different genotypes determined by AAT‐deletion mutation at codon 248 of the BCO2 gene and to find out whether differences in the concentrations of the above compounds in the tissues and milk of the does affect reproduction parameters and the rearing rate of kittens. The experimental materials comprised 36 does, 12 of each genotype of the BCO2 gene, with their litters. Females with their litters were placed in individual cages, on deep litter. Between days 7 and 13 of lactation, samples of milk were collected from the does. The kittens stayed with their mothers until 35 days of age. After weaning, the does were sacrificed. Tissue samples of liver and perirenal fat were collected for chemical analyses. Additionally, based on samples taken from one female, RNA expression levels were determined from the mammary gland and liver, adipose tissue and skin. It was found that homozygous does with deletion at codon 248 of the BCO2 gene were characterized by considerably higher concentrations of xanthophylls and beta‐carotene in the liver, adipose tissue and milk than does with the remaining genotypes. However, the differences in the content of the above compounds in milk had no influence on litter weight or the number and rearing rate of kittens. Additionally, RNA expression of the BCO2 gene was found in the mammary tissue of lactating doe and its level was similar to those noted in the liver and adipose tissue.