浅谈园林施工过程中大树移植——技术要点

大树移植在当今的园林绿化建设中已经较为普遍,但大树移植过程中还存在着各种值得注意的问题,如大树移植的时间、大树移植前的准备工作、起苗包装的方法、大树的吊运、大树的定植等。本文正是通过对上述问题的综合分析,结合自身多年的工作经验,总结大树移植的技术要点。     

Effect of SnoN protein on high-glucose-induced expression of fibronectin in primary cultured rat renal tubular cells

AIM: To investigate the effect of Ski-related novel protein N(SnoN) on high-glucose-induced expression of fibronetin (FN) in primary cultured rat renal tubular cells (RTECs). METHODS: The primary renal cells were cultured, and the cell types were indentified to be RTECs. The cells were divided into 3 groups: normal-glucose group (DMEM+2% FBS), high-glucose group (19.5 mmol/L D-glucose+DMEM+2% FBS) and high-osmotic group (19.5 mmol/L D-mannitol+DMEM+2% FBS). The cells were harvested at 30 min, 2 h, 12 h, 24 h, 48 h, 72 h and 96 h. SnoN expression in primary cultured RTECs was knocked down by RNA interference, then the cells were divided into 4 groups: normal-glucose group, high-glucose group, control siRNA group and SnoN siRNA group. The protein expressions of SnoN and FN in RTECs was examined by the methods of Western blotting, immunocytochemistry staining and immunofluorescence cytochemistry. RT-PCR was used to examine the mRNA expression of FN and SnoN. RESULTS: The RTECs constituted the major cell type of cultured cells. SnoN protein was decreased in a time-dependent manner in RTECs under high-glucose condition. The FN protein and mRNA levels raised in high-glucose group and sustained through entire experiment. Moderate reduction of SnoN in RTECs was observed by RNAi strategy, which greatly up-regulated the expression of FN (P<0.05). CONCLUSION: The down-regulation of SnoN participates high-glucose-induced expression of FN in RTECs.     

Change of serum CXC chemokine ligand 16 in gout patients and its clinical significance

AIM: To explore the relationship between the changes of serum CXC chemokine ligand 16 (CXCL16) and the loss of kidney functions in chronic gout patients and its clinical significance. METHODS: Twenty gout patients with chronic kidney disease (CKD), 22 gout patients without CKD and 22 CKD subjects were recruited into the present study, while 20 normal age-and sex-matched subjects were assigned into the control group. Serum level of CXCL16 and other relevant clinical and biochemical parameters in all subjects were obtained upon standard clinical examinations. Ceatinine clearance rate (CCR) and estimated glomerular filtration rate (eGFR) were calculated based on the clinical parameters. To analyze the clinical data, student's unpaired t-test was used for the comparison between 2 groups. One-way ANOVA assay and multiple stepwise regression were used for multiple groups.RESULTS: Serum level of CXCL16 was significantly increased in gout subjects compared with the healthy control and CKD subjects (P<0.05). Serum level of CXCL16 in gout patients with CKD was significantly higher than that in gout patients without CKD (P<0.05). Furthermore, the mRNA expression of CXCL16 in peripheral blood mononuclear cells (PBMC) of gout patients was significantly higher than that in healthy subjects. Multiple stepwise regression analysis indicated that serum CXCL16 was independently associated with 24 h urine protein, CCR and C-reactive protein (P<0.05). CONCLUSION: Serum CXCL16 level in gout patients is associated with the change of renal functions. Elucidating the pathophysiologcial mechanism of CXCL16 in gout patients requires further study.     

Effect of angiotensin-(1-7) on angiotensin II-induced rat renal interstitial fibroblast activation

AIM: To explore the influence of angiotensin-(1-7) on angiotension II (Ang II)-induced activation and extracellular matrix secretion in rat renal interstitial fibroblasts (NRK-49F cells). METHODS: The NRK-49F cells were maintained and sub-cultured, then the cells were divided into control group, Ang II group, Ang-(1-7) group and Ang II+Ang-(1-7) group. The expression of α-smooth muscle actin(α-SMA),transforming growth factor β₁ (TGF-β₁) and insulin-like growth factor I(IGF-I) was detected by the method of immunocytochemistry when the cells were cultured for 72 h. The content of TGF-β₁, IGF-I and collagen type I(Col I) in the cultured supernatants were measured by ELISA. RESULTS: In control group and Ang-(1-7) group, only basic expression of α-SMA and almost no expression of TGF-β₁, IGF-I and Col I were observed. Compared with control group, the expression of α-SMA, TGF-β₁, IGF-I and Col I was increased in Ang II group. Compared with Ang II group, the expression of α-SMA, TGF-β₁, IGF-I and Col I was significantly decreased in Ang II+Ang-(1-7) group.CONCLUSION: Ang-(1-7) inhibits the activation of renal interstitial fibroblasts and decreases the Ang II induced secretion of Col I by suppressing TGF-β₁ and IGF-I expression.     

高寒区地膜覆盖育苗移栽技术对鲜食玉米生长及鲜穗产量的影响

为了探究覆膜育苗移栽技术对鲜食玉米生长及鲜穗产量的影响,以覆膜直播为对照,分析比较了2种栽培方式对鲜食玉米出苗(成活)率、生育进程、株高、穗位高及穗部性状、鲜穗产量的影响。试验结果表明:覆膜育苗移栽的有效苗率较直播提高15.8%,且生育进程明显早于直播,鲜穗收获期提前;覆膜育苗移栽可使株高和穗位高降低,对穗长及鲜穗产量的影响不明显,但对秃尖的影响极显著。对鲜穗产量影响不明显的主要原因是覆膜育苗移栽使生育进程提前,有效灌浆期延长,百粒重增加。随着相关配套技术的应用,鲜食玉米覆膜育苗移栽技术在晋北高寒地区的优势突显,很好的推广这一技术将是该地区鲜食玉米产业发展的一次革命性突破。     

2ZBX-4型吊杯式蔬菜移栽机的研究与设计

针对我国当前蔬菜移栽机存在的栽植质量差的问题,设计了2ZBX-4型吊杯式蔬菜移栽机,阐述了该机工作原理并分析了工作过程。使用时由拖拉机牵引,人工投苗,可实现半自动化移栽,通过更换从动链轮可对株距进行调节。对2ZBX-4型吊杯式蔬菜移栽机进行了田间试验,结果表明:栽植株距和移栽深度稳定,可以进行多种蔬菜的移栽,损伤率、漏栽率低,直立度好,工作效率高,能满足不同地区、不同蔬菜的移栽农艺要求。     

兽医眼表移植学及植片材料的研究进展

角膜疾病是动物最常见的眼科疾病之一,治疗不当将引起视力减退、致盲甚至眼球摘除,给动物带来巨大的痛苦与生活障碍.兽医眼表移植手术经历了多年的研究探索与改良,成为治疗动物角膜损伤、挽救眼球甚至恢复视力的有效方法.不同的手术方式与移植材料对于角膜愈合、免疫排斥、光学性以及眼球形态产生深远的意义和影响.现就各种眼表移植技术以及...     

SONOGRAPHIC AND SCINTIGRAPHIC EVALUATION OF ACUTE RENAL ALLOGRAFT REJECTION IN CATS

The sonographic features of acute renal allograft rejection in humans and dogs are manifested by increase in renal cross-sectional area and reduction in renal cortical blood flow. These changes have not been investigated in cats. The objectives of this study were to evaluate sonographic and scintigraphic changes during acute renal allograft rejection in cats. Eight SPF, intact, adult, male cats received heterotopic renal allotransplantations. Immunosuppressive doses of cyclosporine and prednisolone were administered for 14 days and then discontinued to allow acute allograft rejection to occur. Serial measurements of renal cross-sectional area, resistive index (RI), echogenicity, and glomerular filtration rate (GFR) were performed to evaluate changes during acute rejection. Upon sonographic confirmation of absent diastolic blood flow or a 20% increase in cross-sectional area of the allograft, a nephrectomy and histopathologic evaluation were performed. Acute allograft rejection was confirmed histologically in all cats. Significant increases in renal cross-sectional area (P < 0.001) occurred postoperatively and during rejection. There were no significant changes in RI (P = 0.43) at any time. A subjective increase in medullary echogenicity and a decrease in corticomedullary demarcation were observed in the rejection period. While GFR decreased significantly in the immediate postoperative period (P < 0.001), no further change occurred during rejection (P = 0.42). Changes in RI and GFR do not appear to be sensitive indicators of acute renal allograft rejection in cats. Serial measurements of renal cross-sectional area appear to be a sensitive method for the early diagnosis of allograft rejection in feline renal transplant recipients.