长期施肥对红壤磷组分及活性酸的影响

以12年的红壤长期肥力监测定位试验不同处理土壤为材料,研究了连续施肥对土壤磷组分、土壤对磷吸附解吸、土壤活性酸铝的影响。连续施用化学磷肥和化肥加有机肥,均可提高土壤全磷、无机磷数量。施用有机肥料,土壤中的磷以Ca-P和Al-P积累为主要表现形式,化学磷肥的施用能够提高土壤的全磷,并以Al-P增幅为最大,在所有处理中均表现为土壤O-P相对稳定。有机肥料处理土壤对外源磷的吸附强度明显少于施用化学磷肥和不施用磷肥的处理,有机肥料能够显著提高吸附磷的再利用,在NPKM处理中解吸磷占吸附磷的47.72%,M处理中占42.89%,其它处理中解吸磷占吸附磷数量一般少于8%。MNPK、M处理的PFI为2.51、2.69比N、NP处理4.53、4.37明显降低。在红壤旱地长期施用化学肥料,土壤交换性酸铝成倍增加,土壤酸化严重;施用有机肥料、有机肥料与化学肥料配合施用,土壤交换酸铝表现稳定。     

提高套袋红富士苹果成果率的技术

套袋是当前生产优质高档苹果的重要措施,然而一些潜在病虫害伴随着套袋而加重发生,导致套袋红富士苹果成果率普遍较低。针对影响套袋成果率的主要因素,结合近几年的实践经验,我们提出了"三选、一紧、三推迟"的措施。     

小熊猫源大肠杆菌的药敏特性及致病性

为探明死亡圈养小熊猫(Ailurus fulgens)体内分离大肠杆菌(Escherichia coli,MT820501)的耐药情况及致病性,对分离菌株进行药敏特性分析,同时以小鼠感染实验检测其致病性。结果显示:该分离菌株对哌拉西林、头孢西丁、头孢曲松、头孢吡肟、美罗培南、哌拉西林-他唑巴坦、氨苄青霉素-舒巴坦、卡那霉素、丁胺卡那、新霉素、米诺环素、左氧氟沙星、呋喃唑酮、多黏菌素B敏感,对大部分β-内酰胺类药物及少部分氨基糖苷类药物耐药;小鼠感染实验结果显示,分离菌株半数致死量为8.42×10⁷cfu/m L,死亡小鼠体内分离出与小熊猫体内一致的菌株,病理表现为各实质脏器出现不同程度水肿、充血及炎性细胞浸润,与死亡小熊猫病理损伤特点高度相似,但不完全相同,这可能与感染方式、种属差异、耐受性等相关。     

鱼粉质量对中国对虾、真鲷、饲料转化率和蛋白质消化率的影响

IntroductionInherent to the practice of intensive aquaculture is the generation of wastes having immediate and verybroad effects on the aquatic environment.There is a growing consensus about the need to reduce waste pro-duction in aquaculture to minimize the negative impacts on the environment.Nitrogen(N) and phosphorus(P) wastes are major concerns for many aquaculture operations.In the past,formulation of diets in intensive aquaculture was often aimed at meeting requirements formaximum growt…     

真鲷虹彩病毒自然感染杂交石斑鱼“褐龙斑”的组织病理分析

褐龙斑是雌性褐石斑鱼(Epinephelus bruneus)和雄性鞍带石斑鱼(E. lanceolatus)杂交产生的子代。作为杂交石斑鱼的新品种,国内外尚没有褐龙斑疾病的报道。2017年7月,某养殖场褐龙斑出现急性死亡,10 d内累积死亡率高达80%。现场调查发现,病鱼外观无明显异常,但反应迟钝,伏底死亡。临床检查和剖检可见脾和肾严重肿大、易碎。组织病理切片观察发现,各组织中存在数量不等的嗜碱性、细胞质均一、直径为10~15 µm的肿大细胞。超薄组织切片中发现,肿大细胞胞质内存在大量直径为130~150 nm的虹彩病毒样颗粒。使用特异性的PCR引物,从病鱼脾、头肾等组织中均检测到真鲷虹彩病毒(Red seabream iridovirus, RSIV)的高强度感染。测定了该病毒主要衣壳蛋白(Major capsid protein, MCP)基因1362 bp的全长编码区,构建了19种(株)虹彩病毒系统发育树,结果显示,该病毒属于虹彩病毒科肿大细胞病毒属RSIV类群。本研究首次描述了褐龙斑虹彩病毒病的组织病理特征,揭示了褐龙斑是RSIV新的敏感宿主,为杂交石斑鱼病毒病的诊断与防治提供了重要的参考依据。     

长期施肥对红壤磷组分及活性酸的影响

以12年的红壤长期肥力监测定位试验不同处理土壤为材料,研究了连续施肥对土壤磷组分、土壤对磷吸附解吸、土壤活性酸铝的影响。连续施用化学磷肥和化肥加有机肥,均可提高土壤全磷、无机磷数量。施用有机肥料,土壤中的磷以Ca-P和Al-P积累为主要表现形式,化学磷肥的施用能够提高土壤的全磷,并以Al-P增幅为最大,在所有处理中均表现为土壤O-P相对稳定。有机肥料处理土壤对外源磷的吸附强度明显少于施用化学磷肥和不施用磷肥的处理,有机肥料能够显著提高吸附磷的再利用,在NPKM处理中解吸磷占吸附磷的47.72%,M处理中占42.89%,其它处理中解吸磷占吸附磷数量一般少于8%。MNPK、M处理的PFI为2.51、2.69比N、NP处理4.53、4.37明显降低。在红壤旱地长期施用化学肥料,土壤交换性酸铝成倍增加,土壤酸化严重;施用有机肥料、有机肥料与化学肥料配合施用,土壤交换酸铝表现稳定。     

红壤旱地施用保水剂和稻草覆盖对花生生长和产量的效应

在江西丘陵红壤坡地上进行的试验结果表明:施用保水剂和稻草覆盖均可促进花生的生长发育,明显提高花生的产量,但对花生的出苗率影响不大.施用保水剂、覆盖稻草以及两者叠加处理分别比空白对照增加花生产量26.6%、7.69%和29.1%,其中以单施保水剂处理的经济效益最佳,比对照增加纯收入6330.60元/hm2.     

Continuous Monitoring of Visible and Near-Infrared Band Reflectance from a Rice Paddy for Determining Nitrogen Uptake Using Digital Cameras

Abstract

A two-band digital imaging system —one band for the visible red band (RED, 630?670 nm) and the other for the near infrared band (NIR, 820?900 nm)— was devised and positioned at a height of 12 m above a rice field of 300 m² in area during the 2007 growing season. The imaging system automatically logged bird’seye view images at 10-min intervals from 0800?1600 every day. Radiometric corrections for the pairs of two-band images were done using solar irradiance sensors and preceding calibrations to calculate daily band-reflectance and the normalized difference vegetation index (NDVI) values for 9 plots of rice plants, with 3 levels of planting density and basal fertilization. The daily- averaged reflectance values in the RED and the NIR bands showed different but smooth seasonal changing patterns according to the growth of plants. At the maximum tiller number and the panicle formation stages, the RED and NIR reflectance values had correlation coefficients (r) of 0.79 and 0.81 with above-ground nitrogen absorption per unit land area (NA, g m-²), respectively, whereas the NDVI using the two band reflectance values showed r-value of -0.13. An empirically derived equation for the NA using two band reflectance values showed r-value of 0.96 and a root mean square of error (RMSE) 0.5 g m^–2 (10% of the mean observed NA) in the estimation for the original (not validated) data set acquired at the maximum tiller number and the panicle formation stages. The results indicated that reflectance observation in the RED and NIR bands using the digital imaging system was potentially effective for assessing rice growth.     

外源铅在红壤中的迁移和形态转化

[目的]为污染土壤的控制和修复治理提供科学依据。[方法]采用实验室土柱法,研究外源铅在红壤中的迁移和形态转化。[结果]在水分不饱和条件下,外源水溶性铅进入红壤90 d后并未向15～30 cm土层迁移,而是集中在0～15 cm层次;外源水溶性铅进入红壤(0～15 cm)30 d后水溶态迅速向其他形态转化,其形态分布主要以交换态为主,其次为铁锰氧化物态、残渣态、碳酸盐结合态、有机结合态(未检出)。[结论]外源铅进入红壤90 d后绝大部分还未转化为相对稳定的形态,对土壤—植物系统的污染存在着潜在的危害。红壤(0～15 cm)对外源铅的吸附主要通过离子交换和络合(或螯合)作用。     

Predicting the severity of Dothistroma on Pinus radiata under current climate in New Zealand

Despite being a damaging foliar disease of Pinus species little research has characterised spatial variation in disease severity of Dothistroma needle blight at a macroscale. Using an extensive dataset describing Dothistroma needle blight severity (S_sev) on plantation grown Pinus radiata stands distributed widely across New Zealand the objectives of this research were to (i) develop a regression model describing S_sev, (ii) use this model to identify key drivers of S_sev, their functional form and relative importance, and (iii) develop spatial predictions of S_sev for New Zealand P. radiata under current climate. Using an independent validation dataset, the final model accounted for 72% of the variance in S_sev using four significant (P < 0.001) explanatory variables and an isotrophic exponential model to account for the spatial covariance in the data. S_sev was most sensitive to mean air temperature from November to April (T_Nov-Apr), followed by mean relative humidity from October to April (RH_Oct-Apr), mean total November rainfall (P_Nov), and then stand age (A). There was a quadratic correlation between A and S_sev with S_sev increasing to a maxima at 12 years before declining. S_sev exponentially increased to a threshold with increases in both P_Nov and RH_Oct-Apr. The relationship between S_sev and T_Nov-Apr was quadratic with S_sev increasing to a maximum at T_Nov-Apr of 15.5 °C before declining at higher values of T_Nov-Apr. Spatial predictions of S_sev varied widely throughout New Zealand. Values of S_sev were highest in moderately warm wet environments in the North Island, and on the west coast of the South Island. In contrast, relatively low values of S_sev were predicted in drier eastern and southern regions of New Zealand.