利用HCG放射免疫测试药盒测定牛血浆LH含量

利用人绒毛膜促性腺激素(HCG)双抗体放免药盒直接检测牛血浆促黄体素(bLH)含量,毋需制备受体,仅将说明书介绍的方法略加修改,就可经济、简便地进行测定。用该法测定无情黄牛血浆LH含量为2.3568±1.3570毫微克/毫升(n=11),发情周期第16—17天的母水牛血浆LH含量为3.3283±1.4963毫微克/毫升(n=8)。黄牛注射三合激素、孕马血清促性腺激素(PMSG)和PMSG与三合激素联用后第6小时,血浆LH含量均较处理前显著增高(P<0.05),分别达11.6800±8.6368(n=3),7.1481±5.5316(n=6)和5.4270±4.4053毫微克/毫升(n=4)。水牛注射PMSG后第24小时和48小时,血浆LH含量分别为6.1940±3.3377和5.5483±2.6501毫微克/毫升(n=4)。     

幼龄绵羊血清中T_3含量的季节和昼夜动态变化

幼龄绵羊血清中T_3含量的RIA测定表明,年平均含量1.246±0.49ng/ml。冬季昼夜平均含量最高为1.748±0.13ng/ml,极明显地高于其它三个季度。春季昼夜平均值1.567±0.20ng/ml,也极明显地高于秋、夏季。秋、夏季的昼夜平均值较接近,为0.851±0.06ng/ml和0.816±0.07ng/ml,并无明显差异,但昼夜升降变化,恰好相反。年平均值的昼夜变化及春季含量的昼夜变化,仅见最高值显著高于最低值。其余季度的昼夜含量变化,无明显差异。     

RIA技术在区域自动气象站WEB平台中的应用

从自动气象站的业务实际出发,提出利用RIA技术设计并实现区域自动气象站信息平台（系统）的基本思路。在对RIA技术及其客户端开发技术FLEX详细阐述的基础上,通过引入ArcGIS API for Flex设计实现具有无刷新操作、拥有良好用户体验和数据处理能力的＂区域自动气象站信息平台＂,充分体现了RIA的技术优势。     

Development of hemolymph prostaglandins assay systems and their concentration variations during ovarian maturation in the kuruma prawn, Penaeus japonicus

Hemolymph prostaglandin (PGF₂ and PGE₂) measurement systems for the kuruma prawn Penaeus japonicus were developed and validated. In these systems, both PGF₂ and PGE₂ in hemolymph obtained from a single prawn were measured. PGs were extracted through reverse-phase C18 cartridge column, separated for isomers of each PG by high-performance liquid chromatography (HPLC), and quantified with a radioimmunoassay (RIA). Detectable range was 6.5–200 and 26–400 pg/tube for the PGF₂ and PGE₂ assays, respectively. Serial dilution of extracted and separated hemolymph PGs (RIA sample) produced a curve parallel to the standard curves in both assays. These assays were able to measure PGs in the RIA samples spiked with the authentic standard. Overall, results indicate that the presently established assay systems can accurately measure hemolymph PGs in female kuruma prawns.

When hemolymph PGF₂ and PGE₂ levels were determined and plotted against the gonado-somatic index (GSI), no correlation was found in either PGs. In terms of ovarian developmental stages, however, the concentration of PGF₂ significantly increased from 66.7 pg/ml at stage I (previtellogenic stage) to 303 pg/ml at stage II (primary vitellogenic stage) and decreased gradually thereafter. The concentration of PGE₂ also increased markedly (4037.7 pg/ml) at stage II and decreased at stage III (secondary vitellogenesis stage). These results indicate that PGs vary in concentration during ovarian development in kuruma prawns.     

基于RIA-GIS的农业蓟马害虫分布可视化系统开发

基于全国蓟马分布普查数据,采用RIA-GIS和Web Service技术构建了中国农业蓟马害虫分析可视化系统,实现了蓟马全球分布、普查分布、潜在分布和自定义区域分布等查询功能,为开展农业蓟马害虫分布与防控研究提供了技术支持。     

Biological potency and radioimmunoassay of canine calcitonin

Calcitonin (CT) is a major calcitropic hormone. Because of low cross reactivity of canine CT (cCT) in radioimmunoassays (RIA) developed for other species, a homologous RIA is needed. Synthesis of cCT allowed study of its biologic potency using a rat bioassay and its plasma half-life in dogs. The availability of cCT also made possible the development of a homologous RIA for measurement of basal and stimulated plasma CT concentrations in dogs. The biologic potency of the synthesized cCT in rats is 24 IU/mg of peptide, which is low in comparison with the 4,000 IU/mg of the salmon CT standard. In the dog, an even lower potency of 4.4 IU/mg of cCT was found. Measurement of the disappearance of iv-injected radioiodinated or nonradioiodinated cCT revealed a short biologic half-life of less than 3 min, followed by a long half-life of 20 min. A polyclonal antiserum against synthetic cCT was raised in a goat. Using a final antiserum dilution of 1:12,000 and 125I-labeled synthetic cCT, the RIA had a detection limit of 6.5 ng/l. The antibody did not crossreact with standard human CT and had <0.1% cross reactivity with porcine CT. For measurement of plasma cCT concentrations, an extraction procedure was developed using ethanol. Dilutions of synthetic cCT and canine plasma extracts revealed parallelism over a wide range of concentrations. Size exclusion chromatography of canine plasma extracts on Biogel P-10 revealed a single cCT peak at the same position as [125I]-cCT, showing that there was little interference by other proteins or cCT prohormone. Basal plasma CT concentrations were 12-80 ng/l, and there was an 8- and 20-fold increase after calcium (1 and 2.5 mg/kg body weight) bolus infusion.     

二甲苯胺噻唑对家兔血液、脑脊液、垂体及下丘脑中β－内啡肽浓度的影响

用放射免疫分析法（ＲＩＡ）研究二甲苯胺噻唑（静松灵）对家兔血液、脑脊淮、垂体及下丘脑中β－内啡肽（β－Ｅｐ）浓度的影响。给家兔肌注静松灵（３４ｍｇ／ｋｇ）后，家兔血液及脑脊液中β－Ｅｐ在用药后１．５ｈ增至最高值，即由用药前的７２．０８±４．０５ｐｇ／ｍＬ增至２８９．５４±１０．０４（Ｘ±ＳＥ，ｎ＝５，Ｐ＜０．０１），升高了７５．１１％；脑脊液中β－Ｅｐ在注药后１ｈ增至最高值，即由５３３．４９±１６．５５ｐｇ／ｍＬ增至１９０８．８０±２８．３６ｐｇ／ｍＬ（Ｘ±ＳＥ，ｎ＝５，Ｐ＜０．０１）增高了７２．０８％，经统计分析差异极显著，而对照组，注射前后家兔血液及脑脊液中β－Ｅｐ浓度未见明显变化。给药后家兔垂体及下丘脑中β－Ｅｐ浓度与给药前比较是动态变化规律，垂体中β－Ｅｐ在给药后３０ｍｉｎ降至最低值，即由２４６．６５±４．４２ｐｇ／ｍｇ降至１２０．５０±８．０９ｐｇ／ｍｇ（Ｘ±ＳＥ，ｎ＝３，Ｐ＜０．０１）降低了５１．１５％，以后是回开趋势，给药后３ｈ增至最高值：３３８．６０±１４．７８ｐｇ／ｍｇ，增加了２７．２６％，经统计分析，差异极显著；下丘脑中β－Ｅｐ浓度在给药后３０ｍｉｎ降至最低值，即由３２．１４±１．３０ｐ?     

应用RIA测定奶中孕酮含量诊断奶牛卵巢机能性疾病

正常奶牛一个发情周期由卵泡期、黄体期、黄体生长期和黄体消解期所组成。根据30头正常奶牛的临床观察和测定各个时期的奶中孕酮含量表明,卵泡期奶中孕酮含量为≤5ng/ml,持续时间为4.5±1.5天;黄体期奶中孕酮含量为≥11ng/ml,持续时间为13±2天;黄体生长期奶中孕酮含量为>5ng/ml—<11ng/ml,持续时间为2±1天;黄体消解期奶中孕酮含量为<11ng/ml—75ng/ml,持续时间为1±0.5天.根据这些指标并结合奶牛的临床表现对奶牛的卵巢机能性疾病进行了诊断,获得了较好的结果,提高了诊断的准确率。     

鸵鸟不同繁殖状态下血浆17β-E2和P4水平变化的研究

用RIA法对鸵鸟不同繁殖状态下血浆17β-E2,P4的水平变化规律进行了研究。结果表明,连续产蛋鸵鸟在排卵周期内血浆17β-E2峰值〔(0.932±0.049)ng/mL〕出现在产蛋前10～2h,若在此期间无峰值则表明鸵鸟即将停产;不产蛋鸵鸟血浆17β-E2无显著峰值;3个繁殖状态下鸵鸟血浆17β-E2基础值差异不显著(P>0.05)。连续产蛋鸵鸟在排卵周期内血浆P4峰值〔(8.82±0.49)ng/mL〕出现在产蛋前16～10h,且峰值有较长的持续期,从峰值出现到产蛋期间,血浆P4水平是缓慢下降的;产蛋前血浆P4无峰值表明鸵鸟不再排卵产蛋;不产蛋鸵鸟血浆P4无显著峰值出现,且其基础值比产蛋鸵鸟低,但差异不显著(P>0.05)。     

奶牛分娩前后血浆类固醇激素水平变化与胎衣不下的关系

应用放射免疫分析法（ＲＩＡ）测定了２０头怀孕奶牛（其中胎衣不下（ＲＦＭ）牛和正常（ＮＲＦＭ）牛各１０头］产前１０天到产后２天外周血浆孕酮（Ｐ４）、１７β－雌二醇（１７β－Ｅ２）和雌酮（Ｅ１）的水平。结果表明，产前６～３天，ＲＦＭ牛血浆Ｐ４水平略低于ＮＲＦＭ牛（Ｐ＞０．０５），而于产前２天突然跃升并显著高于ＮＲＦＭ牛（Ｐ＜０．０５）。血浆１７β－Ｅ２水平，ＲＦＭ牛于产前７天到分娩当天显著低于ＮＲＦＭ牛（Ｐ＜０．０５），尤其是产前７天和５天。血浆Ｅ１水平，产前１０～１天ＲＦＭ牛显著低于ＮＲＦＭ牛（Ｐ＜０．０５），尤其是产前８天和４天极显著低于ＮＲＦＭ牛（Ｐ＜０．０１）；而分娩当天，ＲＦＭ牛却显著高于ＮＲＦＭ牛（Ｐ＜０．０５）。此外，Ｐ４／１７β－Ｅ２的比值，在分娩前６天和２天ＲＦＭ牛极显著和显著高于ＮＲＦＭ牛（Ｐ＜０．０１和Ｐ＜０．０５）。上述结果表明，分娩前６天时，雌酮水平显著下降和Ｐ４／１７β－Ｅ２比值显著升高可以作为奶牛胎衣不下的指征。