HPLC法测定复方罗布麻颗粒中槲皮素的含量

[目的]测定复方罗布麻颗粒中槲皮素的含量。[方法]采用反相高效液相色谱法测定,色谱条件为:色谱柱为Zorbax-C18(250 mm×4.6 mm,5μm);流动相为无水甲醇-浓度0.5%磷酸溶液(50∶50,V/V);流速为1.0 ml/min;检测波长为370 nm,柱温30℃;进样量10μl。[结果]槲皮素的平均回收率为100.2%,RSD为1.89%(n=5);含糖型的复方罗布麻颗粒中槲皮素的含量为0.729 5 mg/g,无糖型的复方罗布麻颗粒中槲皮素的含量为0.955 mg/g。[结论]该方法简便、准确、重复性好,可用于复方罗布麻颗粒中槲皮素的含量测定。     

基于RP-HPLC法的金花茶叶中槲皮素含量测定

[目的]建立金花茶叶中槲皮素含量的测定方法。[方法]采用RP—HPLC法测定药材中槲皮素含量。[结果]在3．0—16．7μg/ml的浓度范围内线性关系良好（r=0．9994）,平均回收率为98．27％,RSD=1．18％。[结论]所用方法简便、准确,可用于金花茶药材的质量控制。     

不同炮制方法对虎耳草有效成分含量的影响

[目的]考察不同炮制方法对虎耳草（Saxifraga stolonifera Meerb.）中有效成分含量的影响。[方法]用酒制、酒炒、醋制、醋蒸、清炒、盐制、蜜制和姜制等15种炮制方法炮制虎耳草,并设对照。采用HPLC法分别测定不同炮制方法炮制后虎耳草中有效成分的含量。[结果]与对照相比,醋制1（100 g药材中加入30 g米醋,加热至80℃,炮制时间15 min）炮制的虎耳草样品中岩白菜素的含量最高;醋制2（100 g药材中加入40 g米醋,加热至100℃,炮制时间30 min）炮制的虎耳草样品中原儿茶酸的含量最高;醋制3（100 g药材中加入20 g米醋,加热至80℃,炮制时间15 min）炮制的虎耳草样品中槲皮素的含量最高。综合各种不同的炮制方法,醋制优于其他炮制方法,且以醋制1的效果最好。[结论]该研究可为稳定虎耳草的炮制工艺提供依据。     

Effect of quercetin on proliferation of human ovarian cancer SKOV-3 cells

AIM：To study the inhibitory effect of quercetin on the proliferation and apoptosis of human ovarian cancer SKOV-3 cells. METHODS：SKOV-3 cells were treated with different doses of quercetin. The inhibitory effect of quercetin on the proliferation was detected by MTT assay. The cell apoptosis was determined by immunocytochemistry. The cell cycle and apoptosis were assessed by flow cytometry. RESULTS：Quercetin inhibited the proliferation of ovarian cancer SKOV-3 cells in a time- and dose-dependent manner. Quercetin induced apoptosis of SKOV-3 cells. The proportion of S phase and the apoptotic rate were significantly increased, and the proportion of G2/M phase was reduced after treatment with quercetin. CONCLUSION：Quercetin inhibits the growth of ovarian cancer SKOV-3 cells in vitro, and promotes apoptosis through S phase arrest.     

柠檬桉叶化学成分研究(二报)——黄酮类化合物的分离鉴定

从柠檬桉(Eucalyptus citriodora)叶的醇提取物乙酸乙酯组分中分离提纯了五个黄酮类化合物,经物理和光谱方法鉴定为:槲皮素,杨梅树皮素以及它们的糖苷—槲皮索-3-O-葡萄糖苷,杨梅树皮素的3-O-鼠李糖苷和3-O-葡萄糖苷。此外从乙酸乙酯组分的水解液中,经纸层析检测有槲皮素和杨梅树皮素以及花青定和翠雀定存在,表明该组分中含上述黄酮醇及花青素的苷类化合物。     

荷叶中槲皮素提取工艺研究

[目的]优化荷叶提取槲皮素的工艺。[方法]研究了3种提取方法对槲皮素转移率的影响;通过对荷叶中槲皮素的回流提取条件进行4因素3水平正交试验,确定了荷叶中槲皮素的最佳提取工艺。[结果]采用乙醇回流提取法提取荷叶中槲皮素效果最佳,槲皮素转移率均高于60%,明显优于乙醇温浸法(<16.2%)和碱温浸法(<20.0%)。4因素对于槲皮素转移率的影响程度依次为固液比>溶剂浓度>提取时间>提取温度。荷叶中槲皮素最佳提取工艺条件为:乙醇回流提取,乙醇溶液浓度60%,提取温度100℃,料液比12:0,提取时间1 h。[结论]经试验验证,该研究筛选优化的工艺条件基本稳定、可行。在此工艺条件下,槲皮素的转移率在70%以上。     

RP-HPLC法测定细叶杜香叶中4种活性成分的质量分数

为了建立测定细叶杜香叶中秦皮乙素、秦皮素、金丝桃苷和槲皮素质量分数的方法,采用反相高效液相色谱法(色谱条件:色谱柱为Inertsil ODS-SP(5 μm,4.6mm×250 mm,柱号:9K192609)、流动相为V甲醇)∶V(0.2%醋酸)=43∶57、流速1.0 mL·min-1、检测波长350 nm),测得秦...     

HPLC测定鹿根药材中槲皮素的含量

[目的]建立鹿根药材中槲皮素HPLC含量测定的方法.[方法]以槲皮素为对照品,采用高效液相色谱法,对鹿根药材中槲皮素含量进行测定.流动相:乙腈-1;冰醋酸,色谱柱为 Waters XTerra RP C18(250 mm×4.6 mm,5 μm)色谱柱,流速:1.0 mL/min,检测波长:360 nm,柱温:25℃.[结果]槲皮素在0.086 6～0.866 μg范围内呈良好的线性关系(r=0.999 9),加样回收率为100.68;,RSD为1.18;.[结论]该方法简便、准确、灵敏度高、重现性好,能有效测定鹿根药材中槲皮素含量,可为鹿根药材的质量评价提供科学依据.     

Oral Bioavailability of Quercetin in Horses

Quercetin, one of the most abundant flavonoids in plants, is discussed with respect to health-promoting effects like antioxidative and anti-inflammatory properties. Although most claims regarding biological effects of flavonoids are based on in vitro and ex vivo studies, the use of flavonoid-containing supplements in humans and companion animals has increased in recent years. Flavonoid-containing supplements are also offered for pet and livestock nutrition. However, any systemic effect of a substance within a living subject depends on its bioavailability. Therefore, the aim of the present study was to gain information on the oral bioavailability of quercetin in horses. Four Icelandic horses with a mean body weight (BW) of 315 ± 25 kg (mean ± standard error [SEM]) were fed a test meal (crimped oats 1 g/kg BW) with the addition of quercetin (20 mg/kg BW). Blood samples were collected directly from the jugular vein before and after ingestion of the test meal for 24 hours, and flavonoid content was analyzed by high-performance liquid chromatography. Quercetin was the main metabolite in plasma with intact flavonol structure after β-glucuronidase/sulfatase treatment of blood samples. The area under the plasma concentration–time curve of quercetin accounted for 88% of total flavonols. Forty-seven percent of the quercetin detected in plasma after ingestion of the test meal was not conjugated. In addition to quercetin, the quercetin derivatives isorhamnetin (methylated) and kaempferol were detected in plasma. Although quercetin is orally bioavailable in horses, similar to other monogastric species, the plasma metabolite pattern differs from those found in species investigated previously (rat, dog, pig, and human).