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91.
Jong-Chang Tsai 《Fish physiology and biochemistry》1996,15(4):307-316
The pattern of cytokeratin proteins in the epidermal cells of loach was studied by immunotechniques and partial separation of the epidermal cells. Two monoclonal antibodies, namely 8F7 and 1C45, against the cytokeratin proteins of the loach epidermis were prepared. these two monoclonal antibodies exhibit distinctive results in immunohistochemical staining. The 8F7 monoclonal antibody stains mainly with the epithelial cells, while the 1C45 monoclonal antibody stains specifically with the club cells. The pattern of cytokeratin proteins in the club cells and the epithelial cells of various epidermal layers was further determined by partial separation of these cells. Immunoblotting analysis of the cell fractions confirms the cytokeratin proteins to be differentially expressed in the club cells and the epithelial cells. However, the cytokeratin proteins expressed in the epithelial cells of the basal, middle and outer layers are same. The results indicate that differentiation of the epithelial cells seems limited during their translocation from basal to upper layers, but in those cells that do differentiate into club cells, the cytokeratin pattern changes. 相似文献
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为了将多级旋流分离技术应用于池塘养殖尾水处理,并解决旋流器的水力停留时间短、分离效率低等问题,根据池塘养殖南美白对虾的尾水特点,结合工业标准150旋流分离器的结构参数,设计了一种串联式旋流分离器。该研究基于欧拉-拉格朗日方法,结合雷诺应力湍流模型与离散相模型,建立了串联式旋流分离器的三维两相内流场数值计算模型。在对比试验数据验证计算方法有效性和流场网格独立性的基础上,分析了进水口流量、锥角、颗粒粒径和密度等参数对旋流器的流场特征和分离效率的影响机制,并提出了多级\ 相似文献
94.
Long-term effect of chemical fertilizer,straw, and manure on labile organic matter fractions in a paddy soil 总被引:2,自引:0,他引:2
To assess the effect of long-term fertilization on labile organic matter fractions, we analyzed the C and N mineralization
and C and N content in soil, particulate organic matter (POM), light fraction organic matter (LFOM), and microbial biomass.
Results showed that fertilizer N decreased or did not affect the C and N amounts in soil fractions, except N mineralization
and soil total N. The C and N amounts in soil and its fractions increased with the application of fertilizer PK and rice straw.
Generally, there was no significant difference between fertilizer PK and rice straw. Furthermore, application of manure was
most effective in maintaining soil organic matter and labile organic matter fractions. Soils treated with manure alone had
the highest microbial biomass C and C and N mineralization. A significant correlation was observed between the C content and
N content in soil, POM, LFOM, microbial biomass, or the readily mineralized organic matter. The amounts of POM–N, LFOM–N,
POM–C, and LFOM–C closely correlated with soil organic C or total N content. Microbial biomass N was closely related to the
amounts of POM–N, LFOM–N, POM–C, and LFOM–C, while microbial biomass C was closely related to the amounts of POM–N, POM–C,
and soil total N. These results suggested that microbial biomass C and N closely correlated with POM rather than SOM. Carbon
mineralization was closely related to the amounts of POM–N, POM–C, microbial biomass C, and soil organic C, but no significant
correlation was detected between N mineralization with C or N amounts in soil and its fractions. 相似文献
95.
4LZ-0.8型水稻联合收割机清选装置气固两相分离作业机理 总被引:1,自引:4,他引:1
为解决小型水稻联合收割机脱净率和损失率问题,提高脱粒清选质量,利用两相流动力学理论,分析了4LZ-0.8型水稻联合收割机脱粒清选分流筒中气流和杂物颗粒两相流动的规律。建立了杂物颗粒流的运动微分方程,导出了分离筒中杂物漂浮速度计算的一种方法,通过比较不同粒径、密度的物料的悬浮速度,得到了杂物颗粒最高速度与气流速度之比随气流速度变化的关系曲线,气流和杂物在分流筒及吸风管中运动时的压力损失随气流速度变化呈现先降后升的规律,压力损失中以加速损失和摩擦损失为主,各约占30%和26%。压力损失曲线存在最小值,此时的气流速度定义为经济气流速度。在喂入量为0.8 kg/s,谷草比为3:1脱粒条件下的经济的清选气流速度9.2 m/s,压力损失为630 Pa。该研究为4LZ-0.8型水稻联合收割机脱粒清选部件的参数优化设计及风机的选择提供了理论依据。 相似文献
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OBJECTIVE: To evaluate the efficacy and tolerance of a treatment protocol for obsessive-compulsive disorder, separation anxiety and noise phobia in dogs. DESIGN: A study was undertaken to assess clinical responses in 24 dogs diagnosed with one or more of three behavioural disorders stated above to a treatment regimen that included clomipramine and behaviour modification. PROCEDURE: A detailed behavioural and clinical history was obtained for each dog. Obsessive-compulsive disorder was diagnosed in nine cases: primary presenting complaints were tail-chasing, shadow-chasing, circling and chewing; one case was diagnosed with concurrent separation anxiety. Separation anxiety was diagnosed in 14 cases: presenting complaints included destruction, vocalisation and escaping in the absence of the owner; four cases also exhibited noise phobia. The study also included one dog diagnosed with noise phobia only and another with inappropriate fear responses. Clomipramine was administered orally twice daily. The starting dose was 1 to 2 mg/kg bodyweight. The dose was increased incrementally to a maximum of 4 mg/kg if needed. A behaviour modification program was designed and the owner instructed on its implementation. Dogs continued medication for at least 1 month after clinical signs disappeared or were acceptably reduced, then withdrawal of medication was attempted by decreasing drug dosage at weekly intervals while behaviour modification continued. RESULTS: The presenting clinical sign was largely improved or disappeared in 16 dogs, 5 demonstrated slight to moderate improvement and the behaviour was unchanged in 3. Clomipramine withdrawal was attempted in nine cases: this was successful in five. CONCLUSION: Clomipramine was effective and well-tolerated in controlling signs of obsessive-compulsive disorder and/or separation anxiety and/or noise phobia in 16 of 24 assessable cases, when used in combination with behaviour modification, and improvement in clinical signs was noted in 5 others. 相似文献
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