鹦鹉常见细菌病的病原菌分离与鉴定

鹦鹉饲养过程中常见的细菌病主要包括大肠杆菌病、传染性鼻炎、禽霍乱、传染性眼炎等。通过对鹦鹉常见疫病的病原菌进行分离鉴定,探讨了鹦鹉常见细菌病的主要致病菌的类型以及行之有效的防治方案。试验结果表明,引起鹦鹉常见细菌病的主要致病菌有大肠杆菌、鸡副嗜血杆菌、多杀性巴氏杆菌、金黄色葡萄球菌和耐药性链球菌。     

小孢拟盘多毛孢PM-1菌株的液体发酵及除草活性物质的分离

前期研究发现分离自毛竹的小孢拟盘多毛孢Pestalotiopsis microspora PM-1菌株具有较强的除草活性,为了明确其产生除草活性物质的最佳液体发酵条件,对培养基种类及成分、培养温度、培养时间和培养方式进行了探讨,并利用薄层层析(thin-layer chromatography, TLC)和高效液相色谱(high performance liquid chromatography, HPLC)对除草活性物质进行了分离。小孢拟盘多毛孢在含硫酸亚铁的PD培养基中,30 ℃黑暗静止培养15天,得到的粗提物除草活性最强;除草活性物质经TLC分离获得7个条带,其中条带6具有较强的除草活性,HPLC分析发现该条带含有7个组分,其中组分3对马唐的活性较强,其保留时间为46.3 min。研究结果表明,利用最佳液体发酵条件对PM-1菌株进行发酵培养,可分离获得除草活性化合物——组分3。     

PAN树脂的制备及PAN树脂-微色谱柱分离富集ICP-AES法测定镉的研究

研究了PAN树脂的制备和PAN树脂—微色谱柱对镉的分离富集 ,然后用ICP -AES法测定镉。试验结果表明在pH10的缓冲溶液 (NH3 ·H2 O -NH4Cl)介质中 ,镉被树脂吸附 ,而样品中碱金属、碱土金属不被吸附 ,可用蒸馏水淋洗除去。再用 5mLφ =10 %热盐酸可完全解吸镉等元素 ,用ICP -AES法无干扰地测定ng·g-1级镉元素。     

磁处理棕壤对磷吸附-解吸特性的影响

探讨了磁处理棕壤对磷吸附-解吸的影响,结果表明:外加磁场处理棕壤后磷的吸附量降低,促进了磷的解吸。棕壤不同粒级磁化后(0.002~0.005mm除外)吸附量基本都减少,而<0.002mm粒级和0.005~0.01mm粒级对吸附量减少起主要作用。     

Carbohydrate dynamics in particle-size fractions of sandy spodosols following forest conversion to maize cropping

In southwest France, much of the forested land on sandy spodosols has been converted to continuous maize cropping in the last few decades. To evaluate the impacts of this change on soil organic matter properties, we compared total organic C and neutral and amino sugars content in whole soil and particle size separates of two forested, and five related sites that had been either clear-cut for 12 and 18 mo, or cultivated to maize for 4-22 yr. Soil carbohydrates accounted for 4-7% of the total organic C across all sites. Soil organic C contents of clear-cut and cultivated sites were only 57-79% of the average value measured in forested sites. Accordingly, carbohydrate content of clear-cut and cultivated sites were only 35-66% of the value in forested sites. Ordering the sites in a chronosequence indicated that both total organic C and carbohydrate contents decreased with an increase in time elapsed since clear-cutting and maize cultivation. The only exception was a partial recovery of carbohydrate content in the site that had been under continuous maize for 22 yr. The clay+silt fraction (0-50 μm) was enriched in carbohydrates, mainly of microbial origin, whereas the sand size fractions (50-200 and 200-2000 μm) contained fewer carbohydrates which were mainly of plant origin. Monosaccharide analysis of particle size separates revealed significant differences in carbohydrate composition between sites. Relative to forested sites, the coarse and fine sand fractions in clear-cut and cultivated sites were depleted in carbohydrates and were relatively enriched in plant-derived carbohydrates. Carbohydrate content of the clay+silt fraction drastically decreased upon clear-cutting. Amino sugar content was consistently lower in clear-cut and cultivated sites than in forested sites, indicating that microbial populations were negatively affected by clear-cutting and cultivation. The fungal population appeared more sensitive than bacteria to these land-use changes as indicated by a greater decline in glucosamine than in muramic acid contents.     

散粒农业物料孔口出流成拱机理分析

本文对散粒农业物料孔口出流落粒拱形成的影响因素进行了分析讨论。根据微观力学原理,建立了颗粒成拱的力学模型;并计算了颗粒物料在孔口出流时形成落粒拱的临界条件。导出了粒径与临界孔口直径、拱高之间的关系。在试验的基础上,找出了物料粒径、含水率与临界孔口直径之间的变化规律。对农产品及食品加工机械的设计提供理论依据以及对今后进一步研究有一定意义。     

Sedimentary aggregates in the Peoria Loess of Nebraska, USA

Loess grain size data used to infer transport direction or wind strength are generally derived from vigorously disaggregated samples. However, these data may not adequately represent the effective particle size distribution during loess transport, if the transported dust contained aggregates of fine-grained material. Thin sections of minimally altered C and BC horizons in the late Pleistocene Peoria Loess of Nebraska, USA, indicate the presence of aggregates with diameters of 30–1000 μm. The larger aggregates (>250 μm) are unlikely to have been transported, and are interpreted as the result of soil faunal activity and other pedogenic processes after deposition. Aggregates smaller than 250 μm could have a similar origin, but laser diffraction particle size analysis suggests that many are sedimentary particles. Comparison of minimally and fully dispersed particle size distributions from each sampling site was used to estimate the modal diameter of aggregates. The aggregate modal diameter becomes finer with decreasing loess thickness, representing increasing distance from the source. A similar trend was observed in the modal diameter of fully dispersed particle size distributions, which represents the mode of sand and silt transported as individual grains. We interpret both trends as the result of sorting during transport, supporting the interpretation that many of the aggregates were transported rather than formed in place. Aggregate content appears to increase with distance from the source, explaining a much more rapid downwind increase in clay content than would be expected if clay were transported as particles smaller than 2 μm diameter. Although the Peoria Loess of Nebraska contains sedimentary aggregates, many of the coarse silt and sand grains in this loess were transported as primary particles, were thoroughly exposed to sunlight and are potentially well suited for luminescence dating.     

河北省表层土壤可侵蚀性K值评估与分析

利用河北土系调查成果中的土壤颗粒组成、土地利用及土壤化学性质等资料,利用EPIC模型中土壤可蚀性K值算法以及结合地统计学方法,研究了不同土壤类型、不同质地及不同土地利用类型的土壤可侵蚀性K值和土壤可侵蚀性K值的空间变异特征。结果表明:1河北土壤可侵蚀性K值平均为0.27,其变化范围为0.12~0.40,土壤可蚀性K值在0.30~0.35之间易蚀性土壤面积占总土地面积的63.71%,土壤可蚀性K值在0.25~0.3之间较易蚀性土壤面积占总土地面积的21.52%,这说明该省易蚀性土壤面积较大。2不同质地的K值之间显著性差异,粉砂黏壤质的可侵蚀性K值最大,为0.37;壤砂质的可侵蚀性K值最小,为0.13。而在不同的土地利用类型之间的K值差异性不显著,耕地的K值最大,为0.33;草地的K值最小,为0.22。3河北土壤可侵蚀性K值存在较强的变异性,其变异系数为29%。因此,在土壤侵蚀定量监测、评价水土流失时,应考虑土壤可蚀性K值的这种空间变异状况。块金值/基台值为37.3%,表明在变程内具有中等强度的空间相关性。步长为23 km,变程为440 km,变程远大于步长,表明在小流域尺度下有较好的空间相关性,进行Kriging插值能得到较准确的结果。4河北土壤可蚀性K值大体呈现南高北低的空间分布特征,南部主要是耕作栽培区,北部主要是自然植被区。该研究结果为宏观大尺度土壤资源可持续利用与制定水土保持规划提供科学依据。     

大孔树脂分离纯化柚皮黄酮的研究

该文以总黄酮含量和总黄酮回收率为考察指标，研究了大孔树脂分离纯化柚皮黄酮的工艺。结果表明：AB-8型树脂对柚皮总黄酮有较好的吸附分离性能，是分离纯化柚皮黄酮的适宜大孔树脂；AB-8型大孔树脂分离纯化柚皮黄酮的最佳工艺条件为：柱体积为160 mL，柚皮提取物上样量为62.5 mg/mL(以湿树脂体积计)，先用pH 4的水淋洗，再用30%的乙醇洗脱，洗脱剂用量为2.5～3倍湿树脂体积。AB-8大孔树脂按上述确定的吸附洗脱条件可重复使用3次。柚皮黄酮经上述工艺纯化后总黄酮含量达到39.67%，总黄酮回收率为62.48%。对经AB-8大孔树脂纯化后的柚皮黄酮进行高效液相色谱(HPLC)分析发现，柚皮黄酮主要为黄酮甙类。     

水流驱动下不同灌草格局对泥沙分选特征的影响

泥沙的颗粒大小分布体现了土壤侵蚀的基本信息,分析泥沙分选特征有助于解释坡面土壤的侵蚀过程及其作用机理。通过野外冲刷试验(流量为15,20,30L/min),以砒砂岩区灌丛位于坡上(SU)、坡中(SM)、坡下(SL)的灌草地和无灌丛草地(GL)为研究对象,裸地(BL)为对照,探讨水流驱动条件不同灌草格局泥沙分选特征及其作用机理。结果表明:(1)植被可以通过影响水动力参数来影响泥沙颗粒的分布,不同格局泥沙颗粒随冲刷时间及流量的增大呈粗化趋势,粉、砂粒是该区侵蚀泥沙的主要颗粒。(2)水流剪切力、水流功率和单位水流功率均与泥沙DV呈负相关关系,与D0、D1呈正相关关系;3种冲刷流量下,泥沙DV均值分别为2.422,2.381和2.348,不同灌草格局下DV表现为GL相似文献