The Structure and Sequence Analysis of TLR4 Gene in Cattle

Toll-like receptor 4 （TLR4） is essential for initiating the innate response to lipopolysaccharide （LPS） from Gram-negative bacteria by acting as a signal transducting receptor. In order to help in investigating TLR4 as a candidate disease-resistance gene in cows, we isolated the cDNA （GenBank accession no. DQ839566） by RT-PCR and rapid amplification of cDNA ends （RACE） experiments and analyzed the sequence characters by bioinformatics. The results showed that cattle TLR4 gene about 3 739 bp contains an open reading frame of 2 526 bp encoded 841 amino acids （aa）, 470 bp 5′ untranslated region （UTR）, and 743 bp 3′ UTR. Tissue expression profile by RT-PCR indicated that TLR4 gene expresses in mammary glands, liver, muscle, duodenum, fats, uterus, kidneys, hearts, lungs, pancreas, and ovary. TLR4 protein domain predicted by bioinformatics consists of signal peptide, transmembrane helices domain, 3 sorts of leucine-rich repeat domains （LRR, LRR-TYP, and LRRCT）, and a toll-interleukinl-resistance domain （TIR）. Leucine-rich repeat domains were related with recognizing a broad of pathogen-associated molecular patterns （PAMP） from pathogen, and TIR domain for downstream signaling transduction was most conservative （98% identify） than other domains after alignment of protein from ovine, porcine, human, and mouse. In addition, a 470 bp 5′-flanking region sequence was amplified by PCR, and 15 putative DNA binding sites were predicted, but this sequence lacks TATA box, CCAAT character, and GC-rich regions.     

牛IgG Fc受体Ⅱ胞外区在大肠杆菌中的表达及特性鉴定

从牛肺巨噬细胞cDNA文库中扩增编码牛IgGFc受体Ⅱ(boFcγRⅡ)胞外区基因,PCR产物经EcoRⅠ和HindⅢ双酶切后,插入原核表达载体pET28a的T7启动子下游,构建重组表达质粒pETboRⅡ。以pETboRⅡ转化大肠杆菌BL21(DE3),IPTG诱导表达。SDS-PAGE分析诱导表达菌体可见分子量约为27kDa的蛋白条带,该表达蛋白在细胞质中主要以不溶性包涵体形式存在。Westernblotting和Dotblot检测表明,boFcγRⅡ重组蛋白在变性条件下可与牛IgG特异结合,提示牛FcγRⅡ胞外区可能存在IgG线性结合表位。     

Boundary Element Methods with Non-overlapping Doma in Decomposition for Elastodynamics

This paper presents the numerical implementation of boundary element method incorporated into a non overlapping domain decomposition method for solving the Navier equations of linear elastodynamics problems by Fourier transformation. Several examples are presented.     

A General Program for Transforming an Arbitrary Polynominal Matrix into Smith's Canonical Form and Its Application

To analyze and design multivariable control system by modern frequency domain method, there should be a transformation of an arbitrary polynominal matrix into Smith's canonical form. This paper discusses the treating method and technique in programming and gives a N-S flow diagram of program to transform an arbitrary polynominal matrix into Smith's canonical form. Two transformed and applied examples show, that the program is correct, simple, useful and has good suitability. It is applicable to frequency-domain analysis and design of multivariable system.     

Effect of elevated carbon dioxide and water stress on gas exchange and water use efficiency in corn

CO₂ has been predicted to increase in the future, and thus leading to possible changes in precipitation patterns. The objectives of this study were to investigate water use and canopy level photosynthesis of corn plants, and to quantify water use efficiency in corn plants under two different CO₂ levels combined with four different water stress levels. Corn plants were planted in sunlit plant growth chambers and a day/night temperature of (28/18 °C) was applied. From 21 days after emergence (DAE), the eight treatments including two levels of carbon dioxide concentrations (400 and 800 μmol mol⁻¹) and four levels of water stress (well-watered control, “mild”, “moderate”, and “severe” water stress) treatments at each CO₂ level were imposed. Height, number of leaves, leaf lengths, and growth stages of corn plants were monitored from nine plants twice a week. Corn plants were separately collected, dried, and analyzed for the biomass accumulation at 21 and 60 DAE. Soil water contents were monitored by a time domain reflectometry (TDR) system (15 probes per chamber). The “breaking points” (changes from high to low rates of soil water uptake) were observed in the bottom of soil depth for the water stressed conditions, and the “breaking points” under ambient CO₂ appeared 6-9 days earlier than under elevated CO₂. Although approximately 20-49% less water was applied for the elevated CO₂ treatments than for ambient CO₂ from 21 DAE, higher soil water contents were recorded under elevated CO₂ than under ambient CO₂. However, corn growth variables such as height, leaf area, and biomass accumulation were not significantly different in CO₂ or water stressed treatments. This result may be explained by considering that significant differences in canopy level gross photosynthesis among the water stress treatments was observed only toward the end of the experiment. The higher soil water contents observed under elevated CO₂ resulted mainly from less water use than under ambient CO_2. WUE (above ground biomass per water use since 21 DAE) at the final harvest was consistently higher and varied with a smaller range under elevated CO₂ than under ambient CO₂. This study suggests that less water will be required for corn under high-CO₂ environment in the future than at present.     

基于小波变换的木材声发射驻波信号特征研究

为研究木材损伤断裂时的声发射(AE)信号所激发的驻波信号特征与木材固有特性之间的关系,采用薄木条折断的方式产生AE源,在小波变换的基础上分析驻波频率,并计算纵波传播速率,依据弹性波理论计算出木材顺纹弹性模量(MOE)。首先,在2种不同长度的木材试件一端分别加工出8根80 mm×10 mm的薄木条,通过外加冲击力折断木条以产生AE源,通过放置在试件端面的2个传感器采集原始AE信号,采样频率设定为500 kHz。然后,根据驻波特性确定原始信号的驻波阶段,进而对该阶段AE信号进行4层小波分解,依据分解后信号的时频域特征析取驻波信号波形。最后,依据驻波产生原理计算纵波传播速率,并结合弹性波理论计算试件的MOE。结果表明,拉伸试验测得樟子松和榉木试件的MOE分别为9.30 GPa和11.63 GPa, 800 mm樟子松和榉木试件通过驻波计算所得MOE分别为9.37 GPa和12.34 GPa,与实测MOE的误差分别为0.75%和5.24%;600 mm的樟子松和榉木试件通过驻波计算所得MOE分别为9.31 GPa和11.81 GPa,与实测MOE的误差分别为0.10%和1.55%。     

基于大涡模拟的射流自吸式离心泵非定常流动分析

为了研究射流自吸式离心泵的非定常流动特性,选取内置射流喷嘴的自吸式离心泵作为研究对象,在定常数值计算的基础上采用大涡模拟技术对其进行了非定常数值求解,获得了全流场的流动信息,以第4圈收敛后的流场为例,分析了1个周期内不同时刻下的流场变化规律,并监测射流器内监测点的压力脉动,通过快速傅里叶变换(FFT)分析了监测点的频域特性.结果表明:在1个旋转周期内低压区始终位于射流器直线段,高压区位于泵腔内;靠近叶轮进口位置监测点的压力脉动值要小于靠近喷嘴附近的值;设计工况下,各个监测点的压力脉动最强频率在290.06 Hz附近,监测点的数值模拟主频与理论计算所得的转频与叶频略有偏差,压力脉动集中在fn~3f_n区间的低频区域.     

Simultaneous measurement of soil water and soil hardness using a modified time domain reflectometry probe and a conventional cone penetrometer

Soil strength and water content are important indices for assessing soil resistance to root growth and soil compaction both of which affect other soil properties. Therefore, simultaneous measurement of soil penetration resistance (PR) and soil water content can aid agricultural land management. We measured PR with a conventional cone penetrometer, followed immediately by determining water content using a modified TDR probe inserted into the penetrometer hole. From the results of a field feasibility test, soil water content was measured satisfactorily and correlated well with data obtained by the gravimetric method, except for those data from near the surface owing to soil disturbance when the cone penetrometer was extracted after the PR measurements. Field results demonstrate that PR and soil water content have three‐dimensional variability, with a markedly different distribution pattern between cultivated and subsoil layers at the field scale. Overall, the variability in the PR and soil water data is similar to that reported in previous studies. We conclude that our method produces results helpful to field management of soil and water because it is based on a simple and easy technique for the simultaneous measurement of soil water content and PR.     

汽车悬架的二自由度建模方法及分析

采用二自由度1/4车辆模型对汽车悬架进行了建模分析。首先建立了微分方程和状态空间模型,其次使用了频域响应法和拉氏变换法分析了模型,并绘制了二者的频率响应曲线图,验证了模型的准确性。这为后续的位移、速度、加速度和力反馈控制奠定了数学模型基础。