Effects of Prostaglandin,N-methyl-D-aspartate and Letrozole on Ewes Plasma Levels of Reproductive Hormones were Evaluated on the Basis of Medroxyprogesterone Acetate Supplementation

This study was aimed to investigate the effects of the different compatibility of medroxyprogesterone acetate (MPA) with prostaglandin(PG), N-methyl-D-aspartate (NMDA) and letrozole (LE) on plasma related hormones levels of reproductive ewes. Thirty-nine and 3-year-old multiparity Suffolk ewes with average weight (75.51+11.55)kg were selected,and assigned into 3 groups,test group Ⅰ:Basis diet+34 mg/d MPA+1 mL/ewe PG, test group Ⅱ:Basis diet+34 mg/d MPA+600 mg/d NMDA, test group Ⅲ:Basis diet+34 mg/d MPA+ 14 mg/d LE, respectively, the trial period lasted for 18 days. The results showed as follows:On the basis of supplemental feeding MPA with PG treated,the levels of FSH in plasma of ewes were extremely significantly increased (P<0.01); Treated with NMDA, the levels of E₂, MLT,FSH and LH in plasma of ewes were extremely significantly increased (P<0.01), respectively;Treated with LE, the levels of MLT,LH and T in plasma of ewes were extremely significantly increased (P<0.01), respectively. This study suggested that MPA+NMDA and MPA+LE could be used as medicines for regulate and control oestrus of ewes, and the effect of MPA+NMDA was the best.     

高产抗病杂交水稻新组合菲优600的选育与应用

菲优600是中国水稻研究所与四川内江杂交水稻科技开发中心联合用菲改A与抗病、高配合力恢复系R600配组而成的籼型高产、抗病杂交水稻新组合.该组合表现优势强、丰产性好、抗稻瘟病、适应性广、制种产量高等特点,于2004年5月通过浙江省农作物品种审定委员会审定.介绍了其选育过程、特征特性、栽培及制种技术要点.     

哈密瓜细菌性果斑病菌悬液浓度计测及离体叶接种方法的研究

本实验通过研究得出哈密瓜细菌性果斑病菌(Acidovorax avenae subsp. Citrulli)菌悬液平板菌落计数浓度(CFU)与分光光度计测的混浊度(OD600)成直线关系,回归方程为y=4×10-10X 0.1128,相关系数R2=0.996。对供试的13个哈密瓜不同品种分别用离体叶人工接种和常规的苗期人工接种鉴定了其对果斑病的抗性。结果表明,离体叶人工接种同样可以反应哈密瓜品种的固有抗性。品种的抗病性采用病斑面积占叶面积的百分数(F)来衡量,计算公式:F=((N×d2)/(L×W))×100%其中N为病斑数,d为病斑直径,L为叶长,W为叶宽。与传统的抗性评价标准病情指数     

橡胶树热研7-20-59、PR107、RRIM600品种生理特性比较

[目的]研究橡胶树高产品种热研7-20-59的生理特性,为该品种生产应用时设计适宜的割胶制度提供理论依据,保证其高产稳产。[方法]以热研7-20-59、PR107、RRIM600等3个橡胶品种为试验材料,采用胶乳生理诊断方法,比较不同品种的干胶产量及各项生理指标。[结果]3个品种的年株干胶产量、胶乳干胶含量、总固形物含量、蔗糖含量、硫醇含量从大到小顺序均为PR107、热研7-20-59、RRIM600,无机磷含量、镁离子含量从大到小顺序为热研7-20-59、PR107、RRIM600。[结论]热研7-20-59胶乳再生能力强,蔗糖利用率高,乳管代谢活性强,是一个综合性状优良的高产品种,可适当采用乙烯利刺激。     

基于LC/MS的伊犁马3600 m速度赛赛前、赛后血浆代谢组学差异变化

【目的】研究伊犁马3 600 m速度赛赛前赛后血浆中代谢物的差异变化。【方法】根据马场提供的遗传系谱,挑选5匹(3♂+2♀)无亲缘关系、体重(384.40±34.18)kg、年龄为2岁的速度赛用伊犁马,每日进行等强度训练。第25 d晨饲后4 h进行3 600 m速度赛,试验马以(11.75±0.87)m/s的平均速度完成比赛。赛前安稳状态下和赛后即刻测定心率和呼吸频率,采集血液、制备血浆样品,采集血浆的质谱,采用非靶向性代谢组学方法测定代谢物。【结果】与赛前心率(39.80±0.40)bpm、呼吸频率(14.00±1.80)bpm相比,3 600 m速度赛赛后即刻试验马心率(80.80±7.70)bpm、呼吸频率(78.80±3.90)bpm显著升高(P<0.05)。与赛前相比,试验马赛后即刻部分血浆代谢物产生显著变化,差异代谢物主要涉及柠檬酸循环、丙氨酸、天冬氨酸和谷氨酸代谢、五糖和葡萄糖醛酸间转化、半胱氨酸与蛋氨酸代谢、氧化磷酸化、脂肪酸降解、鞘脂代谢等生化代谢途径(P<0.05)。【结论】伊犁马3 600 m速度赛前、赛后血浆样本的LC/MS代谢谱存在差异,差异代谢物...     

Relationship Between Ca2+-CaM and Ethylene-Induced PG Activity in Tomato Fruit

Polygalacturonase (PG) was studied during ripening and senescence of postharvest tomatofruit at pink stage at low and normal temperature. The results showed that the PG activity increased, thendecreased during ripening and senescence of tomato. Low temperature inhibited but ethylene enhanced PGactivity. Ethylene also enhanced caimodulin content, which was dependent on Ca2+ concentration in cell.When EGTA(Ca2+ chelator), verapamil (Vp) and LaCl3 (Ca2+ channel blockers), trifluoperazine and chloro-promaize (two CaM antagonisms) were used to treat tomato fruit at green mature stage with ethylene, theycould reverse ethylene-induced increase in PG activity, but Vp, chloropromaize (CPZ), trifluoperazine(TFP) could not directly influence PG activity, which indirectly indicated that influx of Ca2+ from the ex-tracellular space including the cell wall via the Ca2+ channel localized in plasma membrane and CaM were re-quired for ethylene-induced PG activity increase and that ethylene signal transduction may be related to Ca2+- CaM messenger system.     

甜瓜多聚半乳糖醛酸酶反义基因植物表达载体的构建

选育耐贮运品种是解决甜瓜贮运难题的有效途径之一.用特异引物从含甜瓜多聚半乳糖醛酸酶(PG)cDNA全序列的pCMPG质粒中克隆出PG基因,将该基因连接至pGEM-T Easy载体,获得重组质粒pGEM-PG.先用SacI和XbaI双酶切pGEM-PG,再用SacI和XbaI消化pBI121,得到除去了GUS基因的线状载体.从pGEM-PG质粒上切下的PG1 cDNA片段的粘性末端和pBI121线状载体的粘性末端恰好反向匹配,构建了甜瓜PG1 cDNA反义序列植物表达载体.     

反义ACS转基因乙烯缺陷型番茄的生理特性

反义 ACC合成酶 (ACS)基因番茄的采后生理性状与普通番茄不同 ,该番茄的果实和叶片乙烯以及果实呼吸强度受到抑制 ,果实乙烯释放量为 0 ,没有出现呼吸高峰 ,呼吸强度在 10～ 14 mg.kg-1.h-1之间波动 ,极显著低于对照 (高峰时为 2 4 .55mg.kg-1.h-1)。反义番茄的叶绿素降解和番茄红素的合成亦受阻 ,果实在采后和贮藏期间逐渐变为黄色或桔黄色而番茄红素的合成很少 ,到采后 30 d仅有 0 .0 4 A.g-1(fw)。乙烯催熟采后 30 d的反义番茄 ,番茄红素增加 6 0倍。反义番茄 PG酶活性在采后缓慢上升 ,到采收后的第 4 5天达到最高为 4 5.16μmol.g-1.h-1,但其活性也仅为普通番茄的一半。乙烯利催熟前后 ,反义番茄的 PG酶活性没有显著性变化     

不同处理对水蜜桃果实PG酶动力学影响的研究

[目的]探讨不同处理对PG酶的影响,为预测水蜜桃贮藏时间奠定基础。[方法]采用硫代硫酸钠滴定法测定PG酶活性,采用LIPS软件进行相关分析和无重复双因子方差分析。[结果]在整个贮藏过程中,PG酶活性呈上升趋势,PG酶活性随时间变化的方程为：VPG=（k）^1/1＋b·t^-b/1＋b;t=0时刻为采果前528h。冷藏条件下PG酶活性变化方程为：VPG=0．026×（528＋T）^1.0974。;不同的处理影响了反应常数k和反应级数b,各处理的k、b值发生了改变。[结论]通过不同处理证明了水蜜桃果实软化受PG酶影响,建立了水蜜桃果实的PC酶动力学模型。     

不同树龄橡胶RRIM600养分含量比较研究

以不同年龄阶段的橡胶无性系RRIM600为研究材料,对各器官(树叶、鲜茎、树根、树皮、树干、胶乳)中五大养分元素N、P、K、Ca、Mg的含量进行研究。结果表明：(1)各养分元素在各器官中的高低顺序,树叶为：N＞K＞Ca＞Mg＞P;树枝为：K＞Ca＞N＞P＞Mg;树根为：Ca＞N＞K＞Mg＞P;树皮为：Ca＞K＞N＞Mg＞P;树干为：N＞K＞Ca＞Mg＞P;胶乳为：N＞K＞P＞Mg＞Ca。橡胶树各养分的高低顺序为：N＞Ca＞K＞Mg＞P。(2)不同器官所含养分的高低顺序,N含量高低顺序为：树叶＞树根＞树枝＞胶乳＞树皮＞树干;P为：树叶＞树枝＞树根＞胶乳＞树干＞树皮;K为：树叶＞树枝＞树根＞树皮＞胶乳＞树干;Ca为：树皮＞树根＞树枝＞树叶＞树干＞胶乳;Mg为：树叶＞树根＞树皮＞树枝＞树干＞胶乳。不同器官养分总含量高低顺序为：树叶＞树皮＞树根＞树枝＞胶乳＞树干。(3)各器官养分含量与月份和树龄之间建立的直线回归均达显著差异,橡胶RRIM600树叶、树枝、树皮、树干和胶乳养分含量主要受月份影响,树根主要受树龄影响;N、P、K、Ca、Mg均主要受月份影响。