民族“口碑文献信息”之传承与搜集

从图书馆开展民族＂口碑文献信息＂搜集之文化背景;民族＂口碑文献信息＂传承模式之依赖以及对民族＂口碑文献信息＂传承模式之分析等三方面开展理性思考,期望对民族＂口碑文献信息＂的搜集工作有所裨益。     

口含烟中主要成分和游离烟碱含量的多元分析

为考察口含烟中主要成分对游离烟碱含量的影响,以41个Moist Snuff类型口含烟为实验材料,采用相关分析、回归分析和通径分析法探讨口含烟中含水率、pH值、烟碱对游离烟碱含量的影响.得到如下结果:(1)相关性分析表明,pH值、烟碱与游离态烟碱呈正相关,且相关系数分别达5％显著和1％极显著性水平,而含水率与游离烟碱相关...     

基于农村生源高职生英语口语教学的创新攻略

论述了提高英语口语能力的必要性与迫切性,以及目前高职非英语专业农村生源学生的口语能力相对低下的问题,并提出了提高非英语专业农村高职生英语口语能力的若干创新举措。     

Feed intake and oral behaviour of dairy calves housed individually or in groups in warm or cold buildings

The influence of environmental temperature, housing, social company and age on the feed intake and oral behaviours was studied by housing male dairy calves for seven weeks individually (INDIV) or 12 weeks in groups of four; indoors (INGROUP) or outdoors either with (OUTWARM) or without a heated shelter (OUTCOLD). During the milk-feeding period (1–7 experimental weeks), the mean daily milk, hay and total dry matter intakes of INDIV calves were lower than of INGROUP calves (P < 0.05). No differences in the feed intake between OUTWARM, OUTCOLD or INGROUP were found. Nor was found any differences in the feed efficiency (growth kg^− 1 DM) between the treatments. After weaning off milk, during experimental weeks 8 to 11, the total dry matter intake of INGROUP tended to be greater than that of OUTWARM (P = 0.05). There were no other differences in the mean daily feed intakes, nor in the feed efficiency. During the milk-feeding period, INGROUP calves ate more often concentrates (P = 0.05) and ruminated more (P = 0.01) than INDIV calves. During the 12-week experiment, INGROUP calves licked themselves more often than OUTCOLD calves (P = 0.04) and tended to lick more often than OUTWARM calves (P = 0.08). INGROUP calves licked more often penmates or structures and bit more often structures than OUTWARM or OUTCOLD calves (P < 0.05 for all). Overall, with a decreasing temperature, the calves in outside groups spent less time performing oral behaviours. We conclude that group rearing may facilitate calves to start eating and ruminating earlier than individual housing. The effect of low temperatures on the time spent eating solid foods is discussed in relation to housing design.     

农业类高职院校英语口语教学现状分析及对策研究

通过分析农业类高职院校英语口语教学的现状和存在的问题,提出相应的解决对策,从而激发学生的学习主动性,提高教师的教学质量和学生的学习效果.     

表达猪细小病毒VP2蛋白的重组干酪乳杆菌诱导小鼠产生特异性抗体

 【目的】研究以干酪乳杆菌作为传递抗原活载体表达猪细小病毒主要免疫保护性抗原VP2蛋白的免疫特性。【方法】将构建的重组猪细小病毒（PPV）VP2基因的细胞表面表达型载体pPG-VP2电转化干酪乳杆菌L.casei 393,获得阳性重组菌pPG-VP2/L.casei 393。重组菌以2%乳糖为诱导物,在MRS培养基中进行诱导,经SDS-PAGE、Western blot及间接免疫荧光分析,目的蛋白获得表达。将重组菌及空质粒菌株分别口服接种Balb/c小鼠,收集粪便及肠黏液样品测定小鼠产生抗VP2的特异性sIgA,采集血液样品测定小鼠产生抗VP2的特异性IgG,并对获得的抗体进行PPV中和活性的测定。【结果】重组干酪乳杆菌pPG-VP2/L.casei393免疫小鼠能够产生明显的抗PPV的sIgA和IgG抗体水平,其对PPV的中和效价分别为1﹕24和1﹕128。【结论】为PPV重组乳酸菌口服疫苗的研制提供了重要的物质基础。