Comparative development of mouse tooth germs transplanted in subrenal capsule and oral submucosa

AIM: To compare 2 environments, the subrenal capsule and oral submucosa, for producing well-formed teeth from mouse tooth germs and for exploring the ideal environment for tooth regeneration. METHODS: Two groups were set up. Group A was transplanted with the mouse embronic day (ED) 14.5 first mandibular molar tooth germs into the subrenal capsule, while group B was transplanted with the ED14.5 first mandibular molar tooth germs into the oral submucosa. After 3 weeks and 4 weeks, the host mice were sacrificed, and the transplanted explants were evaluated with morphologic observation, histological structures, hardness and elastictic modulus tests, and chemical compositions. RESULTS: (1) The explants isolated from both environments showed the tooth-like structures, but as to the group B, the crown was smaller, and the shape of the cusps was not significant. (2) HE staining showed that the dentin and enamel in group A were thicker than those in group B in which the ameloblasts and odontoblasts were differentiated not very well. (3) In the test of enamel hardness, only the hardness of 4 weeks in group B was lower than normal mouse tooth. In the test of enamel modulus, the elastic modulus of enamel in 3 weeks of group A was slightly lower than normal mouse tooth, but the difference was not significant.The elastic modulus of enamel in 4 weeks of group A and group B was significantly lower than normal mouse tooth and 3 weeks of group B. The hardness and elastic modulus of dentin in 3 groups was not significant. (4) Raman spectroscopy showed 2 groups grew in harmony in general, they all had the largest peak in the point of 961 cm^-1, but the 3 weeks of group B had an obvious peak in the point of 2 947 cm^-1. CONCLUSION: For the development of ED14.5 tooth germs, we obtain almost the whole tooth in subrenal capsule transplantation after 3 or 4 weeks. The buccal submucosa environment still has a certain influence on the tooth germ development, although there are some differences about the tooth development between this environment and subrenal capsule environment.     

道路检测数据处理软件的开发

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考试是教学工作中的一个极其重要的环节。为了加强对考试环节的监督,更好的发挥考试的指导作用、反馈作用,必须制定科学合理的试卷标准评估体系。通过对试卷标准评估体系的指标构建、实施过程进行分析,找出在考试过程中存在的问题及薄弱环节,使教师在教学过程中有针对性的进行改进,促进教学质量不断提高。     

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流行性蚜霉菌的安全性研究

流行性蚜霉菌是棉蚜等蚜虫的流行性病原体。为了给扩大生产和推广使用本菌防治蚜虫提供依据,本研究对其田间应用的流行性蚜霉菌-258型菌剂进行了安全性试验。用小白鼠、豚鼠和雏鸡经不同途径接种不同剂量的孢子作急性、亚急性和慢性试验,结果表明:试验动物均未表现有临床症状,实验前后增重和红、白细胞变化均无显著性差异(P>0.05),各组织器官经病理学检查和扫描电镜观察也无异常。由此证明E.f-258对哺乳动物和禽类是安全的。     

钻孔压浆桩的应用及效果

阐述了钻孔压浆桩的施工及特点，单桩承栽力的取值，结合工程应用实例，分析了桩基检测的结果，并提出了应注意的问题。     

学生考试分数的几种分析方法

对学生考试分数的分析方法有图形分析法、项目分析法和整体分析法。图形分析法是通过作考试分数的频率分布图来完成；项目分析法则是针对试题和试卷的难度而言；整体分析法是在建立数学模型的基础上，从信度和效度两方面加以测量，一般要求信度在0．9以上，效度在0．8以上。