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排序方式: 共有184条查询结果,搜索用时 15 毫秒
81.
82.
为验证新药清营口服液的安全性和有效性,进行了Wistar大鼠的安全性试验和SPF鸡临床疗效试验。在40~10 g/kg b.w.剂量范围内,经口给予清营口服液30 d后,检查Wistar大鼠的一般生理状态及血液生理、生化指标,并进行病理解剖及病理组织学检查。结果表明清营口服液各剂量组所有检测指标,与阴性对照组无显著差异(P0.05),且对Wistar大鼠的生长发育未产生影响,较长期重复应用无亚急性毒性,说明该药安全性较强:通过对比清营口服液试验组和氟苯尼考对照组对SPF鸡的疗效发现清营口服液组能够对体内注射的大肠杆菌起到较好的抑菌和杀菌效果,从试验鸡的死亡率和分菌结果可以看出清营口服液总体预防和治疗效果好于氟苯尼考,说明清营口服液有较好的临床抗菌效果。 相似文献
83.
D.D. Miceli M.F. Cabrera Blatter M.F. Gallelli O.P. Pignataro V.A. Castillo 《Research in veterinary science》2014
The incretin glucagon-like peptide 1 (GLP-1) enhances insulin secretion. The aim of this study was to assess GLP-1, glucose and insulin concentrations, Homeostatic Model Assessment (HOMAinsulin sensitivity and HOMAβ-cell function) in dogs with pituitary-dependent hyperadrenocorticism (PDH), and compare these values with those in normal and obese dogs. The Oral Glucose Tolerance Test was performed and the glucose, GLP-1 and insulin concentrations were evaluated at baseline, and after 15, 30, 60 and 120 minutes. Both basal concentration and those corresponding to the subsequent times, for glucose, GLP-1 and insulin, were statistically elevated in PDH dogs compared to the other groups. Insulin followed a similar behaviour together with variations of GLP-1. HOMAinsulin sensitivity was statistically decreased and HOMAβ-cell function increased in dogs with PDH. The higher concentrations of GLP-1 in PDH could play an important role in the impairment of pancreatic β-cells thus predisposing to diabetes mellitus. 相似文献
84.
85.
The present study examines the responses of broiler chickens to oral administration of Clostridium perfringens freshly isolated from field cases of necrotic enteritis (NE). The challenge studies included long-term exposure and short-term exposure, factored in with dietary and management variables including high levels of dietary components such as fish meal, meat meal, abrupt change of feed, and fasting. In the long-term exposure trials, the birds were orally inoculated daily, with 1 ml (1.0 or 2 x 10(8) CFU/ml) of an overnight culture of C. perfringens for 7 days. Short-term exposure trials involved challenge with 1 ml (3 x 10(10) CFU/ml) administered as a single dose. The responses of broilers to orally administered C. perfingens under laboratory controlled conditions are presented and discussed in the context of authentic field cases of necrotic enteritis. None of the challenge trials produced overt clinical signs of NE and there were no mortalities associated with oral exposure to high doses of C. perfringens. However, many of the challenged birds showed distinctly pronounced pathological changes in the intestinal tissue. On gross examination the responses in birds challenged orally with C. perfringens could be placed into two categories: (1) no apparent pathological changes in the intestinal tissue and (2) sub-clinical inflammatory responses with focal, multi-focal, locally extensive, or disseminated distribution throughout various sections of duodenum, jejunum, ileum, and ceca. In birds that responded with intestinal lesions, hyperemia and occasional hemorrhages were the main gross changes. In some birds, the mucosa was covered with a brownish material, but typically, the mucosa was lined by yellow or greenish, loosely adherent material. Mild gross changes were seen in some control birds, but both qualitatively and quantitatively, the lesions were distinctly more pronounced in the challenged birds. Upon histological examination, none of the experimentally exposed birds showed overt mucosal necrosis typical of field cases of NE, but typically the lamina propria was hyperemic and infiltrated with numerous inflammatory cells. Most significant changes were seen at the interface of the basal domain of enterocytes and lamina propria. Multifocally, these areas were extensively edematous, allowing for the substantial disturbance of the structural integrity between the lamina propria and the enterocytes. The lesions observed in the present study were consistently reproduced in all of our challenge trials, hence these responses may signify newly emerging patterns of sub-clinical enteric disorders in contemporary strains of poultry. The pathological changes observed in broilers challenged orally with C. perfringens in the present study, differ significantly from those reported previously, and must be clearly differentiated from those described in cases of NE or ulcerative enteritis. Although no overt necrosis of the intestinal mucosa typical of field cases of NE were observed in the present study, the birds challenged with C. perfringens showed strong inflammatory reaction to the introduced pathogens. The distinct features of the microscopic lesions were changes involving apparently normal enterocytes at the interface of the basal domain of villar epithelia and lamina propria. Although the pathological changes in the intestinal tissues observed in our trials appear to be rather subtle when compared to field cases of NE, the nature of these lesions suggest a significant negative effect on the digestive physiology of intestinal mucosa. 相似文献
86.
Wilson M Moshitzky P Laor E Ghanim M Horowitz AR Morin S 《Pest management science》2007,63(8):761-768
Pyriproxyfen, a juvenile hormone (JH) mimic, is a biorational insecticide that disrupts insect development. It is one of the principal insecticides being used to control Bemisia tabaci (Gennadius) on cotton, and has many environmentally positive attributes that make it compatible with integrated pest management (IPM) programs. In Israel, a high level of resistance to pyriproxyfen has been observed in several isolated regions. Here, tests were conducted to establish whether temporal refuges from exposure to pyriproxyfen could be useful for restoring the effectiveness of the compound. Resistance was found to decrease by a factor of 8 when exposure to pyriproxyfen was ceased for 13 generations. Reversal of resistance was accompanied with increased biotic fitness of the revertant colony. By incorporating experimental estimates of nymph survival, sex ratio, fecundity, egg hatching rate and developmental time, the seasonal cost per generation for resistant insects was estimated to be 25%. A genetic simulation model, optimized by empirical data from bioassays, predicted fitness cost per generation of 19% for resistant homozygous (RR) females and hemizygous (R) males, and produced rates of reversal similar to the experimental results. The model also predicted that, even after 5 years ( approximately 55 generations) without pyriproxyfen treatments, the frequency of the resistance allele (R) will still remain high (0.02). It is therefore concluded, on the basis of experimental and modeling results, that the effectiveness of temporal refuges for reversing development of resistance to pyriproxyfen in B. tabaci may be limited. 相似文献
87.
Thompson HM Wilkins S Battersby AH Waite RJ Wilkinson D 《Pest management science》2007,63(11):1081-1084
Systems have been developed to monitor the direct effects of insect growth regulator (IGR) pesticide exposure on honey bee development, but there has been little work on the longer-term impact of exposure on the colony. A honey bee population model provided the opportunity to investigate the effects of short-term mortality of brood and of sublethal changes in behaviour of the surviving adults on honey bee populations. The model showed that brood mortality alone has limited effect on colony size. There were two mechanisms that could have greater influence on productivity. Precocious foraging in affected adult bees, and hence early loss of brood-rearing (nurse) capabilities, had a much larger effect than expected. Increasing mortality rates by 30% to simulate sublethal effects on lifespan, rather than reduced brood-rearing capability, gave a significantly smaller effect. In order to simulate an effect with the 'shortened lifespan' mechanism as large as that with the 'premature ageing' mechanism, the mortality rate of affected adults had to be increased by 500%. A significant finding from the model is that application of IGRs in spring and early summer could have substantial effects on colony size and viability. Sublethal effects such as precocious foraging can have worse effects than massive brood mortality, as it severely reduces the ability to rear the next generation of nurse bees. 相似文献
88.
Vaccination of chickens using raw rice coated with novel trehalose nano-organogels containing Newcastle disease (strain I-2) vaccine 总被引:1,自引:1,他引:0
P. N. Wambura 《Tropical animal health and production》2009,41(5):797-802
The formulation and evaluation of trehalose nano-organogels for storage and oral delivery of Newcastle disease (ND) strain
I-2 vaccine to chickens were carried out in this study. Trehalose sugar was blended with vegetable oil to form nano-organogels
where trehalose also acted as a stabilizer against thermal inactivation of I-2 ND virus. Results from infectivity titration
assay indicated that the titre of 107.5 EID50/0.1 mL was maintained after 12 weeks of storage of nano-organogel I-2 vaccine at ambient room temperature. Serology results
showed that 33% chickens which were vaccinated with nano-organogel I-2 vaccine after 14 days had HI antibody titres of ≥ 3.0 log2 with GMT of 2.3. Moreover, results showed 100% of chickens vaccinated with nano-organogel I-2 vaccine had the mean antibody
titres of 3.4 and 3.7 log2 at 21 and 28 days after vaccination, respectively. All vaccinated chickens (100%) survived the challenge of virulent ND virus
whereas all unvaccinated chickens succumbed to challenge and died of signs consistent with ND. The findings from this study
showed that the nano-organogel I-2 vaccine was stable at room temperature, safe and produced protective antibody response
in vaccinated chickens. Moreover the nano-organogel I-2 vaccine was used for oral administration and hence is suitable for
mass vaccination. However, optimization of the formulation of trehalose nano-organogel vaccine is required in order to achieve
its application potentials. 相似文献
89.
牛小迎 《青海畜牧兽医杂志》2009,39(3):9-10
用EM口服液在果洛地区的牦牛犊中进行了增重试验,同时与消维康口服液、畜痢灵口服液的增重效果进行了比较。结果:EM组、消维康、畜痢灵和对比照组牦牛犊分别比实验前增重31.10±4.0864kg,29.94±4.6441kg,25.98±3.7498ks和19.78±4.2584ks:其中:EM组比对照组平均增重多11.32kg(P〈0.01);比畜痢灵组平均增重多5.12ks(P〈0.05);比消维康组平均增重1.63kg(P〉0.05)。 相似文献
90.
目的建立HPLC法测定喘舒口服液中盐酸麻黄碱的含量。方法色谱柱HypersilC18(4.6mm×250m,5μm),乙腈-0.1%磷酸溶液(含0.1%三乙胺)(5:95)为流动相,流速为1.0ml·min^-1,检测波长为210nm,柱温为30℃。结果实验表明盐酸麻黄碱的含量在9.576—38.304μg.mL。范围内与峰面积呈良好的线性关系(r=0.9990),平均回收率为98.02%,RSD=0.59%(n=6)。结论该方法简便快速,重现性好,可用于喘舒口服液的质量控制。 相似文献