腐殖酸水溶肥配施枯草芽孢杆菌对基质青菜生长的影响

为探究腐殖酸水溶肥、枯草芽孢杆菌对基质青菜生长的影响 本研究以抗热605青菜为试验材料,共设计6组施肥试验,分别为CK1(有机肥2.5g/盆)、CK2(有机肥2.5g/盆+枯草芽孢杆菌0.5g/盆)、T1(有机肥2.5g/盆+枯草芽孢杆菌0.5g/盆+腐殖酸水溶肥100倍液)、T2(有机肥2.5g/盆+枯草芽孢杆菌0.5g/盆+腐殖酸水溶肥200倍液)、T3(有机肥2.5g/盆+枯草芽孢杆菌0.5g/盆+腐殖酸水溶肥400倍液)、T4(有机肥2.5g/盆+枯草芽孢杆菌0.5g/盆+腐殖酸水溶肥800倍液),研究结过表明:腐殖酸水溶肥配施枯草芽孢杆菌可在一定程度上增加基质青菜的株高、叶宽、叶长、冠幅及地上鲜重,其中腐殖酸水溶肥起主导作用,枯草芽孢杆菌作用较为微弱,综合各生长指标考量以T2处理效果最佳。     

对羟基苯甲酸和肉桂酸对巨尾桉9号苗木生长及生理指标的影响

采用基质栽培试验,研究对羟基苯甲酸、肉桂酸对3月生巨尾桉9号苗木生长及叶片抗氧化酶活性的影响,结果表明：对羟基苯甲酸各处理浓度（10、50、100、500 mg/L）下的苗高增长量、地径增长量均显著高于对照,且在10 mg/L时,苗高增长量最大,为52.33 cm;肉桂酸10 mg/L时巨尾桉苗木苗高增长量较对照高,且随着其浓度的增加,苗高增长量和地径增长量均逐渐降低。对羟基苯甲酸、肉桂酸各浓度下巨尾桉9号苗木叶片叶绿素总含量均极显著低于对照,丙二醛含量略高于对照;对羟基苯甲酸、肉桂酸各浓度对巨尾桉9号叶片超氧化物歧化酶（SOD）、过氧化物酶（POD）、多酚氧化酶（PPO）活性均具有一定的影响。对羟基苯甲酸10、50、100 mg/L及肉桂酸10 mg/L处理下的综合化感效应指数为正,对羟基苯甲酸500 mg/L及肉桂酸50、100、500 mg/L处理下的综合化感效应指数为负。可见,对羟基苯甲酸、肉桂酸对巨尾桉9号苗木的生长具有“低浓度促进、高浓度抑制”的规律,拐点浓度分别为100、10 mg/L;且在处理浓度相同时,肉桂酸的化感作用较对羟基苯甲酸强。     

Proliferation-inhibitory and apoptosis-inducing effects of cinnamic acid combined with cisplatin on human hepatocellular carcinoma cell line MHCC97

AIM：To investigate the effects of cinnamic acid (CA) combined with cisplatin on the proliferation and apoptosis of human hepatocellular carcinoma cell line MHCC97. METHODS：Human hepatocellular carcinoma cell line MHCC97 was culured and divided into CA group, cisplatin group, CA+cisplatin group and control group. MTT assay, inverted microscopy, annexin V-FITC/PI staining and flow cytometry were applied to identify the viability, morphology and apoptosis of the cells. The apoptosis-related signaling protein caspase-3 was detected by Western blotting. RESULTS：CA and cisplatin either alone or in combination significantly inhibited the proliferation and induced obvious apoptosis of MHCC97 cells, while CA alone or combined with cisplatin had no significant inhibitory effect on normal human liver L-02 cells. The rates of mid-and late apoptosis or necrosis were higher in cisplatin group than that in CA group or combination group, but the early apoptotic rate was just the opposite. Pro-apoptotic activity in combination group was much stronger than that in CA group or cisplatin group at lower concentration, and combination group promoted apoptosis and decreased the cytotoxic side effects of cisplatin. CA and cisplatin either alone or in combination also up-regulated the cleaved caspase-3 expression in a time-dependent manner, and the effects in CA group and combination group were higher than that in cisplatin group. CONCLUSION：CA and cisplatin either alone or in combination inhibit the growth of MHCC97 cells by inducing apoptosis, and the activation of caspase-3 may play important roles in these processes.     

Biochemical and histological effects of gibberellic acid on Locusta migratoria migratoria fifth instar larvae

Experiments were conducted to assess the effect of gibberellic acid (GA₃), a plant growth regulator, on Locusta migratoria migratoria fifth instar larvae. Newly emerged larvae were exposed to various concentrations of GA₃ administered by topical application or by forced ingestion. Results showed that treated insects exhibited toxic symptoms with a dose-dependent mortality. GA₃ toxicity was also demonstrated by perturbation of the moult processes. In fact, we noted that treated insects present exuviations difficulties due to the impossibility to reject the old integuments causing mortality in the 5th instar larvae. Histological study of proventriculus revealed alterations in the epithelial cells and absence of apolysis phenomenon. Data also showed that GA₃ induced significant quantitative variation of haemolymph metabolites. These changes result in a significant decrease in the total concentration of proteins and carbohydrates and an increase in the total concentration of haemolymph lipids.     

视黄酸及干扰素对胃癌细胞系MKN45中p16,p21,c-myc基因表达的影响

目的探讨全反式视黄酸及干扰素两种因子对胃癌MKN45细胞的影响.方法将视黄酸及干扰素同时加入胃癌细胞系MKN45中进行细胞培养,用MTT法测定细胞的生长状况,并通过Northern blot和免疫组化测定p16,p21及c-myc的表达情况.结果干扰素（IFN）协同全反式视黄酸（ATRA）可有效地抑制MKN45细胞生长,癌细胞经联合用药诱导后p16和p21基因的表达水平提高,c-myc基因表达水平下降.视黄酸受体RARα基因在MKN45细胞中呈低水平表达,经ATRA和IFN诱导后表达水平提高.结论 ATRA联合IFN诱导可调节p16和p21基因的表达水平,抑制胃癌MKN45细胞生长,这可能与细胞中RARα基因高水平表达有一定关系.     

Study on some functional and compositional properties of yak butter lipid

The present experiment was performed to determine some functional properties of yak butter lipids such as lipid class composition; conjugated linoleic acid (CLA) composition, differential scanning calorimetric (DSC) analysis, tyrosinase inhibition activity and antioxidant property. Yak butter lipids composition contained 98% triacylglycerols, 0.9% free fatty acids, 0.32% free sterols and 0.27% phospholipids. The CLA content in yak butter was 2.5% and the major portion was of cis‐9 and trans‐11 (90%). The DSC analysis of yak butter lipids showed a similar path for transition temperature as cow butter lipids, although the enthalpy of yak lipid was higher (40.0 mJ/mg) compared to cow butter lipids (32.0 mJ/mg). Melting point of yak butter was observed at 41°C. Yak butter with lactic acid, NaCl, citric acid and ascorbic acid showed pronounced tyrosinase inhibition activity. Vegetable oils blended with yak butter have extended the oxidation induction time.     

A rapid method for early generation selection of dwarf genotypes in wheat

Summary In the early generations of a breeding programme to incorporate the successful Norin 10 semidwarfing genes into adapted tall or standard lines, the bread and durum wheat breeder must be able to recognise and discard those lines that are not homozygous for the dwarfing gene. A method which exploits the gibberellin insensitive reaction of lines carrying the genes combined with a single seed descent technique is described in which most unwanted genotypes may be removed as F2 seedlings.     

黑麦草叶蛋白提取工艺研究

采用酸化和加热相结合的方法，研究了从黑麦草中提取叶蛋白的最佳工艺条件，并分析了黑麦草叶蛋白中的氨基酸组成。结果表明黑麦草叶蛋白提取的最佳工艺条件为pH4.0，料水比1：2，凝聚温度为70℃，加热时间5min。该条件下提取的叶蛋白含量可高达70.48%。氨基酸分析显示黑麦草叶蛋白中人体必需氨基酸、鲜味氨基酸和药效氨基酸分别占总氨基酸的40.3247%、22.2439%和77.5609%。黑麦草叶蛋白具有很好的营养、保健和药用价值。     

青霉酸毒性研究进展

青霉酸是圆弧青霉代谢产物的主要成分。现已证明,青霉酸对各种动物具有毒性,主要引起心脏、肝脏和肾脏等器官的损伤,并具有潜在致癌性。青霉酸在饲料中的含量很高,而且污染极其广泛。因此,研究青霉酸的毒性、中毒机理及与其他毒素的联合作用,对保证饲料卫生和动物健康具有很重要的意义,该文就青霉酸的器官毒性、细胞毒性、分子毒性及联合毒性的研究进行综述。