黑龙江省鸡西地区奶牛子宫内膜炎病原菌分离鉴定及耐药性研究

从黑龙江省鸡西地区部分奶牛场选取25头临床诊断为子宫内膜炎的患牛,并从这25头患牛体内分离得到39株细菌,其中大肠杆菌17株、葡萄球菌13株、链球菌5株、其他菌4株。耐药性试验表明,大肠杆菌、葡萄球菌和链球菌都对头孢曲松钠和头孢噻呋钠敏感,对庆大霉素比较敏感,对其他几种常用抗生素存在不同程度耐药性。     

某动物医院2017—2018年犬病用药情况分析

[目的]分析某动物医院2017—2018年犬病用药情况,以便掌握周围地区的犬病流行状况及药物使用情况,为临床用药提供基础数据。[方法]采用回顾性研究法,对2017—2018年的4 562张犬病门诊处方中的药物品种、用药金额、用药频度、日均费用、药物利用指数等进行分析。[结果]抗生素药物品种多;肤多乐浴液、聚维酮碘乳膏用药金额较高;地塞米松磷酸钠注射液、复方甘草酸铵注射液、托芬那酸片等用药频度较高;双甲脒销售金额与用药次数同步性好;2017—2018年药物利用指数均小于1。[结论]基于分析结果说明该动物医院犬用药情况基本合理。     

大连地区几种食用贝类药物残留的检测

本文应用化学发光免疫法检测技术检测了大连地区几种食用贝类（海湾扇贝Argopectens irradias、文蛤Meretrix meretrix、青柳蛤Mactra chinenesis、菲律宾蛤仔Ruditapes philippinarum和魁蚶Anadara uropygimelana）体内呋喃唑酮酶、氯霉素酶、恩诺沙星和有机磷的残留。结果表明,所检测的贝类体内均有不同含量的上述几种药物残留,但其残留量均在食用卫生标准的控制下,符合食品卫生安全标准。     

常用免疫抑制剂的研究进展

综述了近年来广泛应用于,1名床的激素类、化学类、真菌类和中草药类免疫抑制剂的发现过程、作用机制、临床应用特点、毒副作用和发展前景.旨在为合理选用免疫抑制类药物和开发高效低毒的新型免疫抑制剂提供一定的参考.     

Effects of Prednisone Alone or Prednisone with Ultralow-Dose Aspirin on the Gastroduodenal Mucosa of Healthy Dogs

Background: The coadministration of prednisone and ultralow-dose aspirin has been recommended for the management of various diseases, but the safety of this combination in dogs has not been studied.
Hypotheses: The gastroduodenal lesions associated with prednisone and ultralow-dose aspirin administration will be similar to those caused by prednisone alone, but both treatments will result in more severe lesions than placebo.
Animals: Eighteen healthy adult purpose-bred dogs.
Methods: Randomized, blinded, placebo-controlled study of 3 treatment groups for 27 days: placebo, prednisone, and prednisone and aspirin. Gastroduodenoscopy was performed before and on days 5, 14, and 27 of treatment and mucosal lesions scores were assigned. Mucosal lesion scores were compared by a Kruskal-Wallis test. Clinical signs were compared by the Friedman's chi-square test (significance at P < .05).
Results: There were no significant differences in the gastroduodenal lesion scores among groups, or within groups at any time during the study. Significantly more dog-days of diarrhea occurred in the prednisone and aspirin group during treatment, compared with baseline. No significant differences in clinical signs were found among any of the groups.
Conclusion: The concurrent use of prednisone and ultralow-dose aspirin did not increase the severity of gastroduodenal lesions compared with prednisone or placebo. Coadministration of prednisone and ultralow-dose aspirin increases the frequency of mild, self-limiting diarrhea in some dogs.     

乙肝病毒X蛋白结合蛋白最新研究进展

乙肝病毒X蛋白结合蛋白（HBXIP）是肝细胞癌变过程的一个关键因素,它能在动物肌肉组织和恶性肿瘤组织中过表达。近年来研究显示：HBXIP能与人体内多种蛋白结合。本文综述了HBXIP蛋白参与细胞凋亡与增殖、细胞周期进程、中心体复制、肿瘤细胞迁移等过程,以期为以HBXIP蛋白为靶标的新的抗乙肝病毒等药物设计提供基础。     

Krempfielins N–P,New Anti-Inflammatory Eunicellins from a Taiwanese Soft Coral Cladiella krempfi

Three new eunicellin-type diterpenoids, krempfielins N–P (1–3), were isolated from a Taiwanese soft coral Cladiella krempfi. The structures of the new metabolites were elucidated by extensive spectroscopic analysis and by comparison with spectroscopic data of related known compounds. Compound 3 exhibited activity to inhibit superoxide anion generation. Both 1 and3, in particular 1, were shown to display significant anti-inflammatory activity by inhibiting the elastase release in FMLP/CB-induced human neutrophils.     

Klymollins T–X,Bioactive Eunicellin-Based Diterpenoids from the Soft Coral Klyxum molle

Five new eunicellin-based diterpenoids, klymollins T–X (1–5), along with two known compounds (6 and 7) have been isolated from the soft coral Klyxum molle. The structures of these new metabolites were elucidated by extensive spectroscopic analysis and by comparison with related known compounds. Compound 5 was found to exert significant in vitro anti-inflammatory activity against LPS-stimulated RAW264.7 macrophage cells. Furthermore, compounds 4 and 7 were shown to exhibit cytotoxicity against a limited panel of human cancer cell lines.     

Delivery of Berberine Using Chitosan/Fucoidan-Taurine Conjugate Nanoparticles for Treatment of Defective Intestinal Epithelial Tight Junction Barrier

Bacterial-derived lipopolysaccharides (LPS) can cause defective intestinal barrier function and play an important role in the development of inflammatory bowel disease. In this study, a nanocarrier based on chitosan and fucoidan was developed for oral delivery of berberine (Ber). A sulfonated fucoidan, fucoidan-taurine (FD-Tau) conjugate, was synthesized and characterized by Fourier transform infrared (FTIR) spectroscopy. The FD-Tau conjugate was self-assembled with berberine and chitosan (CS) to form Ber-loaded CS/FD-Tau complex nanoparticles with high drug loading efficiency. Berberine release from the nanoparticles had fast release in simulated intestinal fluid (SIF, pH 7.4), while the release was slow in simulated gastric fluid (SGF, pH 2.0). The effect of the berberine-loaded nanoparticles in protecting intestinal tight-junction barrier function against nitric oxide and inflammatory cytokines released from LPS-stimulated macrophage was evaluated by determining the transepithelial electrical resistance (TEER) and paracellular permeability of a model macromolecule fluorescein isothiocyanate-dextran (FITC-dextran) in a Caco-2 cells/RAW264.7 cells co-culture system. Inhibition of redistribution of tight junction ZO-1 protein by the nanoparticles was visualized using confocal laser scanning microscopy (CLSM). The results suggest that the nanoparticles may be useful for local delivery of berberine to ameliorate LPS-induced intestinal epithelia tight junction disruption, and that the released berberine can restore barrier function in inflammatory and injured intestinal epithelial.     

果蔬中食源性致病微生物的研究进展

全球食源性疾病的发生呈增长态势,由果蔬中食源性致病微生物引发的疾病也在逐年增加。因此,各国对于果蔬中食源性致病微生物的检测也越来越重视。目前,食源性致病微生物的快速检测和相关的防控技术成为研究的热点,本文综述果蔬中常见大肠杆菌、李斯特菌和沙门氏菌的种类、特性、病症、检测方法及防控技术,为保障食品安全提供参考。