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91.
应用酶联免疫吸附试验法鉴定几种主要马铃薯病毒 总被引:19,自引:2,他引:19
制备了PVX,PVY_o,PVY~n,PVA,PVS,PVM,PLRV和TRV等8种主要马铃薯病毒抗体的辣根过氧化物酶结合物,在检测黑龙江省马铃薯主栽品种克新一、二、三、四号等品种的植株样品1155份和800余份正在脱毒中的试管苗样品中,发现上述8种马铃薯病毒在几个主栽品种中的侵染率较高,而且大多数感病植株是由3至7种病毒复合侵染,并首次查明本省存在PVY~n和PVM病毒。对不带上述8种病毒的样品又用双向反向聚丙烯酰胺凝胶电泳鉴定带马铃薯纺锤块茎类病毒(Potato spindle Tuber Viroid)状况,现已获得无上述8种病毒和类病毒的种植材料。 相似文献
92.
93.
Olsson, S.-O., S. Viring, U. Emanuelson and S.-O. Jacobsson: Calf diseases and mortality in Swedish dairy herds. Acta vet. Scand. 1993,34, 263-269.– A survey of the mortality and morbidity affecting calves during the first 3 months of life was carried out. Results are reported from 131 herds with altogether 5,050 calvings. These herds kept individual calf cards for all calves born. The incidences of abortions and stillborn calves were 0.6% and 3.6%, respectively. During the period 0-90 days the mortality and morbidity were 2.6% and 11.0%, respectively. The frequencies of enteritis and pneumonia in calves were 7.2% and 0.8%. Mortality and morbidity were influenced by breed, season, age of the dam and time of first colostrum. The frequencies of abortions, stillborn calves, mortality and morbidity varied considerably between herds. Herd factors influencing mortality and morbidity during different periods of time were: herd size, yield, zero-grazing, whether the calf was allowed to feed by suckling, the design of the calf pens, and previous incidence of infectious enteritis in the herd. On average, both mortality and morbidity in calves were low, though in certain herds, temporarily high frequencies were registered. 相似文献
94.
Jung K Kang BK Kim JY Shin KS Lee CS Song DS 《Veterinary journal (London, England : 1997)》2008,177(2):231-235
Epidermal growth factor (EGF) promotes gastrointestinal mucosal recovery by stimulating the mitogenic activity of intestinal crypt epithelial cells. The aim of this study was to determine the effects of EGF on atrophic enteritis induced in piglets by experimental infection with porcine epidemic diarrhoea virus (PEDV) strain Dr13. Two groups of 12 conventional, colostrum-deprived, 1-day-old, large White-Duroc cross breed piglets were inoculated orally with PEDV (3 x 10(5) 50% tissue culture infective doses), with or without EGF (10 microg/kg/day, intraperitoneally once daily for 4 days after infection) and compared to 12 uninfected, untreated control piglets. PEDV+EGF piglets had less severe clinical signs than PEDV only piglets at 48 and 60 h post-infection (hpi). Histologically, the ratio of villous height:crypt depth of PEDV+EGF piglets was significantly higher than PEDV only piglets at 36 and 48 hpi. Immunohistochemistry for Ki67 demonstrated increased proliferation in intestinal crypt epithelial cells of PEDV+EGF piglets compared to PEDV only piglets at 36, 48 and 60 hpi. EGF stimulates proliferation of intestinal crypt epithelial cells and promotes recovery from atrophic enteritis in PEDV-infected piglets. 相似文献
95.
鸭坏死性肠炎是由魏氏梭菌引起的一种急性传染病,鸭球虫病是鸭常见且危害十分严重的寄生虫病。这2种病一年四季均可发生,以夏秋季多发。一旦发生鸭群危害甚大,如果2种病混合感染,造成的经济损失就更惨重。一般发生于地面散养的雏鸭,成鸭也有发生。鸭舍场地潮湿、寒冷、遇有阴雨天,污水排除困难,鸭误食误饮不卫生的饲料、废水是本病发生的主要诱因。主要症状就是食欲减少、拉稀、排暗红色或暗黑色混有黏液的恶臭粪便。剖检以小肠中后段的充气、弥散性出血肿胀,后期发展为纤维素性坏死性肠炎,肠黏膜留有圆形白色、红色坏死点为特征。 相似文献
96.
Ketoconazole (Nizoral®, Janssen Pharmaceutica) a new systemic antimycotic was tested in dermatomycosis in cats and dogs. The daily dose (10 mg/kg body weight) was administered for 10 or 20 days without any other measures being taken. After the 20‐day treatments new hair growth was observed in 96.7 per cent of the cats and 89.9 percent of the dogs. Clinical cure was complete in 96.8 per cent of the cats and 90.5 per cent of the dogs. Particularly the good tolerance in the cat was appreciated. There were practically no side‐effects in dogs (except vomiting in two pups) or in cats. 相似文献
97.
复方鱼用中药整肠颗粒的制备及其性能 总被引:1,自引:1,他引:0
用中药材黄柏、黄连、秦皮和白头翁提取物制备了复方鱼用中药整肠颗粒,建立了产品有效成分含量的测定方法,研究了产品的稳定性、安全性和临床应用的有效性.结果表明,利用高效液相色谱法对复方鱼用中药整肠颗粒中主要抑菌活性成分盐酸小檗碱的含量进行检测,其精密度、稳定性、重复性和加样回收率均较好.产品室温留样观察24个月,样品性状、含量等各项指标均无明显变化,稳定性良好.产品对日本鳗鱼、甲鱼、黄鳝和南美白对虾混饲喂服的安全浓度均大于3 g·kg-1;对胃肠炎常见致病菌——肠型点状气单胞菌、鳗弧菌和嗜水气单胞菌的最小抑菌浓度(MIC)均为0.625μg·mL-1,温和气单胞菌的MIC为1.25μg·mL-1.药效试验结果表明,对草鱼因细菌感染产生的急、慢性肠炎,每公斤饲料添加药物2-3 g,连续5-7 d进行治疗,药物平均保护率在45.2%左右. 相似文献
98.
将240只AA肉鸡随机分为3个处理组:对照组、坏死性肠炎(Necrotic enteritis,NE)模型组及乳酸杆菌处理组。28日龄,每组屠宰24只鸡,对各组肉鸡肠道损伤进行肉眼评分,并采集回肠黏膜样本分别用于电镜下形态观察和Real—timePCR检测紧密连接蛋白claudin-1和occludin的表达。结果显示,乳酸杆菌处理组降低了炎症的损伤程度(P〈0.05);电子显微镜观察显示,乳酸杆菌处理组鸡回肠上皮微绒毛较NE模型组有改善;real—timePCR分析显示,乳酸杆菌处理组claudin-1和occludin的表达显著高于NE模型组(P〈0.05),但与对照组没有差异。该试验结果表明,L.fermentum1.2029可降低NE的损伤程度,并能有效上调NE条件下claudin-1和occludin的表达,显示其具有保护肠道屏障功能的作用。 相似文献
99.
A chicken embryo-adapted duck enteritis virus (DEV) strain is the most widely used vaccine against duck virus enteritis (DVE) infection. The kinetics of attenuated DEV vaccine was examined in tissues of ducklings vaccinated by the mucosal or systemic route at 20 days of age and sampled regularly up to 60 days post-vaccination (p.v.). Significant numbers of virus genomes in the lymphoid and other parenchymatous organs were first detected at 60 min p.v., and subsequently rose to peak levels during 90 min to 1 day p.v. independent of the route of vaccine administration. The peak level of vaccine virus in the individual parenchymatous organs of subcutaneously immunized ducklings was significantly higher than that of orally or nasally immunized ducklings. The route of vaccine administration had significant effect on the initial tissue distribution of vaccine virus in respiratory and digestive tracts. Vaccine viruses spread to digestive tract and trachea tissues by mucosal route, i.e. oral and nasal administration, early than that by subcutaneous route. The rapid early increase of vaccine virus levels in all samples examined followed by a steady decline from 90 min to 6 days p.v. The real-time PCR analysis of a variety of tissues is significant for further investigation of the mechanism of vaccinal protection, and the optimization of vaccination regimes. 相似文献
100.