放牧条件下乳用伊犁马采食量的初步研究

2013年8-10月,采用模拟采食法、群牧差额法、内外指示剂结合法在昭苏马场草甸草地上进行了放牧条件下乳用伊犁马采食量的测定。结果表明,1)同种测定方法不同月份之间测定的乳用伊犁马干物质采食量差异不显著(P0.05)。2)模拟采食法测得的干物质采食量为12.79 kg·15 h-1·匹-1,群牧差额法测得为16.39kg·d-1·匹-1,内外指示剂结合法测得为13.28 kg·d-1·匹-1。3)群牧差额法与内外指示剂结合法测定的乳用伊犁马干物质采食量之间差异不显著(P0.05),初步确定乳用伊犁马平均干物质采食量为14.83 kg·d-1·匹-1。     

草业科学在现代马业发展中的研究现状

马是人类社会发展的重要组成部分,人类种草养马历史悠久,但人们对马用饲草的认识和研究还不足,致使现代马业和草业科学发展存在严重脱节现象,影响了两者的可持续发展。因此,结合现代马业发展趋势及其对饲草的利用特点,综述了草地放牧、青干草、青贮及半干青贮饲草等在现代马业发展中的研究与实践现状,并提出了现代马业发展中草业科学存在的问题及建议,以期为保障现代马业的可持续发展提供理论指导,为拓展我国现代草业科学与技术发展空间提供参考。     

Monitoring desertification processes in Mongolian Plateau using MODIS tasseled cap transformation and TGSI time series

Most remote sensing studies assess the desertification using vegetation monitoring method. But it has the insufficient precision of vegetation monitoring for the limited vegetation cover of the desertification region. Therefore, it offers an alternative approach for the desertification research to assess sand dune and sandy land change using remote sensing in the desertification region. In this study, the indices derived from the well-known tasseled cap transformation(TCT), tasseled cap angle(TCA),disturbance index(DI), process indicator(PI), and topsoil grain size index(TGSI) were integrated to monitor and assess the desertification at the thirteen study sites including sand dunes and sandy lands distributed in the Mongolian Plateau(MP) from 2000 to 2015. A decision tree was used to classify the desertification on a regional scale. The average overall accuracy of 2000, 2005, 2010 and 2015 desertification classification was higher than 90%. Results from this study indicated that integration of the advantages of TCA, DI and TGSI could better assess the desertification. During the last 16 years, Badain Jaran Desert, Tengger Desert, and Ulan Buh Desert showed a relative stabilization. Otindag Sandy Land and the deserts of Khar Nuur, Ereen Nuur, Tsagan Nuur, Khongoryn Els, Hobq, and Mu Us showed a slow increasing of desertification, whereas Bayan Gobi, Horqin and Hulun Buir sandy lands showed a slow decreasing of desertification. Compared with the other 11 sites, the fine sand dunes occupied the majority of the Tengger Desert, and the coarse sandy land occupied the majority of the Horqin Sandy Land. Our findings on a three or four years' periodical fluctuated changes in the desertification may possibly reflect changing precipitation and soil moisture in the MP. Further work to link the TCA, DI,TGSI, and PI values with the desertification characteristics is recommended to set the thresholds and improve the assessment accuracy with field investigation.     

Single layer centrifugation of stallion spermatozoa consistently selects the most robust spermatozoa from the rest of the ejaculate in a large sample size

Reasons for performing study: An improvement in sperm quality after single layer centrifugation (SLC) has been seen in previous studies using small sample sizes (for example, n = 10 stallions). There is a need to investigate whether this improvement is repeatable over several breeding seasons with a larger number of stallions (n ≥ 30 stallions). Objective: To make a retrospective analysis of the results of SLC performed on more than 250 sperm samples (176 ejaculates) from 31 stallions in 3 consecutive breeding seasons. Methods: Sperm quality (motility, proportion of morphologically normal spermatozoa and the proportion of spermatozoa with undamaged chromatin) was assessed before and after SLC. Results: All parameters of sperm quality examined were significantly better in sperm samples after SLC than in their unselected counterparts (P<0.001 for each parameter). The yield of spermatozoa obtained after SLC was influenced by the type of extender used and also by the concentration of spermatozoa in the original ejaculate, with fewer spermatozoa being recovered when the loading dose contained a high concentration of spermatozoa. The optimal concentration was approximately 100 × 10⁶/ml. Sperm concentration in the samples loaded on to the colloid influenced the sperm yield while the type of semen extender affected sperm quality and survival. Furthermore, the scaled‐up SLC method was found to be suitable for use with a range of ejaculates, with similar sperm kinematics being observed for standard and scaled‐up preparations. Conclusions: SLC consistently improved the quality of stallion sperm samples from a large number of ejaculates. The method could be scaled‐up, allowing larger volumes of ejaculate to be processed easily from a wide range of stallions.     

Effect of hay type on cecal and fecal microbiome and fermentation parameters in horses

The effect of hay type on the microbiome of the equine gastrointestinal tract is relatively unexplored. Our objective was to characterize the cecal and fecal microbiome of mature horses consuming alfalfa or Smooth Bromegrass (brome) hay. Six cecally cannulated horses were used in a split-plot design run as a crossover in two periods. The whole plot treatment was ad libitum access to brome or alfalfa hay fed over two 21-d acclimation periods with subplots of sampling location (cecum and rectum) and sampling hour. Each acclimation period was followed by a 24-h collection period where cecal and fecal samples were collected every 3 h for analysis of pH and volatile fatty acids (VFA). Fecal and cecal samples were pooled and sent to a commercial lab (MR DNA, Shallowater, TX) for the amplification of the V4 region of the 16S rRNA gene and sequenced using Illumina HiSeq. The main effects of hay on VFA, pH, and taxonomic abundances were analyzed using the MIXED procedure of SAS 9.4 with fixed effects of hay, hour, location, period, and all possible interactions and random effect of horse. Alpha and beta diversities were analyzed using the R Dame package. Horses fed alfalfa had greater fecal than cecal pH (P ≤ 0.05), whereas horses fed brome had greater cecal than fecal pH (P ≤ 0.05). Regardless of hay type, total VFA concentrations were greater (P ≤ 0.05) in the cecum than in feces, and alfalfa resulted in greater (P ≤ 0.05) VFA concentrations than brome in both sampling locations. Alpha diversity was greater (P ≤ 0.05) in fecal compared with cecal samples. Microbial community structure within each sampling location and hay type differed from one another (P ≤ 0.05). Bacteroidetes were greater (P ≤ 0.05) in the cecum compared with the rectum, regardless of hay type. Firmicutes and Firmicutes:Bacteroidetes were greater (P ≤ 0.05) in the feces compared with cecal samples of alfalfa-fed horses. In all, fermentation parameters and bacterial abundances were impacted by hay type and sampling location in the hindgut.     

Genetics of equine bleeding disorders

Genetic bleeding disorders can have a profound impact on a horse's health and athletic career. As such, it is important to understand the mechanisms of these diseases and how they are diagnosed. These diseases include haemophilia A, von Willebrand disease, prekallikrein deficiency, Glanzmann's Thrombasthenia and Atypical Equine Thrombasthenia. Exercise-induced pulmonary haemorrhage also has a proposed genetic component. Genetic mutations have been identified for haemophilia A and Glanzmann's Thrombasthenia in the horse. Mutations are known for von Willebrand disease and prekallikrein deficiency in other species. In the absence of genetic tests, bleeding disorders are typically diagnosed by measuring platelet function, von Willebrand factor, and other coagulation protein levels and activities. For autosomal recessive diseases, genetic testing can prevent the breeding of two carriers.