金顶侧耳菌丝体提取物的动物免疫增强功能初探

本文研究了金顶侧耳对小白鼠腹腔巨噬细胞吞噬功能的作用 ,结果表明 :分别以 2 0mg/kg d、 10 0mg/kg d、剂量的金顶侧耳水提物对小白鼠进行腹腔注射 ,可明显提高小白鼠腹腔巨噬细胞的吞噬能力 ,与对照相比 ,效果极显著 ;但 10mg/kg d的剂量未能引起腹腔巨噬细胞吞噬能力的显著提高     

Dynamic expression of CD30 on peripheral blood lymphocytes in mice after a cecal ligation and puncture

AIM: To investigate the details of Th2 cell differentiation in septic mice. METHODS: Experimental septic mice were induced by cecal ligation and puncture (CLP). The exression of CD30 on CD4⁺T cells at different time after CLP were estimated by flow cytometry following three-color immunofluorescent staining.RESULTS: CD30 expression on CD4⁺T cell was different at each time point. The highest expression was showed at 38 h after CLP and declined later, which matched the changes in mortality of the animals. CONCLUSION: During sepsis, differentiation of Th2 cell changed with the development of sepsis and might be associated with the severity of the disease.     

Effect of Ershen Zeshu Mixture on the peritoneal lymphatic stomata and the ascites drainage

AIM: To further study the effect of Ershen Zeshu Mixture, a Chinese medicine, on the peritoneal lymphatic stomata and the ascites drainage in experimental mice model of CCl₄-induced fibrosis.By the application of NO supplier (sodium nitroprusside,SNP) and NO suppressor (N^G-mononethyl-L-arginine,L-NMMA),the effect of NO on the peritoneal lymphatic stomata and the ascites drainage was studied to clarify the relationship between Ershen Zeshu Mixture and NO in the regulation of the peritoneal lymphatic stomata.METHODS: The mouse model of fibrosis was established with the application of intragastric installations of carbon tetrachloride one time every three days;Scanning electron microscope and computer image processing were used to detect the girth,area and distribution density of the peritoneal lymphatic stomata;the concentration of urinary ions was measured in the experiment.RESULTS: ①Compared to NS group and model group,Ershen Zeshu Mixture could significantly increase the girth and the area of the peritoneal lymphatic stomata,promote its distribution density (P <0.01) and enhance the drainage of urinary ions such as sodium,potassium and chlorine(P<0.05 or P <0.01).②The effect of Ershen Zeshu Mixture on the peritoneal lymphatic stomata was altered by adding NO supplier or NOS suppressor to the peritoneal cavity,that is,NO supplier can enhance the regulation effect of Ershen Zeshu Mixture on the peritoneal lymphatic stomata,but NO suppressor can antagonize the Ershen Zeshu Mixture's effect.CONCLUSIONS: ①These observations indicated that Ershen Zeshu Mixture might regulate the peritoneal lymphatic stomata to drainage materials in the peritoneal cavity.②The effect of Ershen Zeshu Mixture on lymphatic stomata and ascites drainage maybe have relationship with endogenous NO.     

Changes of plasma endostatin levels in nude mice bearing human nasopharyngeal carcinoma

AIM: To approach the changes of endostatin levels in BALB/c nude mice bearing human nasopharyngeal carcinoma(NPC) in different period (5, 10, 20, 30 and 40 days) and the relationship between endostatin and tumor's development. METHODS: BALB/c nude mice bearing NPC was reproduced by hypodermic implantation of human CNE-2 cells into right-side of axillary fossa. The level of plasma endostation was detected, and the weight of isolated tumors was measured. On the basis of the regulation of these changes, their relationships were explored. RESULTS: At 5 days [(137.61±53.41) μg/L] or 10 days [(103.06±17.33) μg/L] endostatin level had no apparent alternation in comparison with control group [(113.56±21.74) μg/L, P>0.05]. At 20, 30 and 40 days concentration of endostatin[(212.80±85.91) μg/L,(293.63±62.53) μg/L, (271.57±32.45) μg/L, respectively] were higher than that of the control group (P<0.05). Along with the development of the tumors, both the levels of endostatin and tumors weight increased. There was a positive correlation between the level of endostatin and tumor weight (r=0.687, P<0.05). CONCLUSION:These results suggested that endostatin links with the development of NPC.     

Progress in studying the mechanism of fever using knockout mice

Increasing evidence suggests that a complex net-work of fever induction pathways in mammalian exists. In this article, the overview of recent studies on the mechanism of fever induced by different pyrogens using IL-1, IL-1R, ICE, IL-1ra, IL-1RacP, IL-6, IL-10, TNFR, cPLA₂, COX, EP, AT₂, iNOS and D_2/3 knockout mice is presented. Hyperthermia respond to localized infection/inflammation (e.g., sc injection of turpentine) is mediated by IL-1β and IL-6 in turn.While fever induced by systemic infection/inflammation (e.g., treatment with LPS intraperitoneally) varies with the different doses of pyrogens administered. Fever caused by a low dose of LPS administered ip is IL-6 dependent, but the IL-6 independent pathway is crucial for the fever evoked by a high dose of LPS. Febrile responses during both local and systemic infection/inflammation develop totally through central PGE₂ dependent mechanism, but some stress induced hyperthermia otherwise.     

肠宁(CN)的急性及蓄积毒性试验研究

为了提供中药肠宁(CN)的安全毒理学评价资料,本试验采用改良Karber法测定小白鼠的 LD50＞60 973 mg/kg;在蓄积毒性试验中,小白鼠未表现出蓄积毒性。这说明中药肠宁属于实际无毒 性物质,并无蓄积毒性作用。     

2种共轭亚油酸异构体对小鼠肌内脂肪沉积和肌肉线粒体代谢的影响

试验旨在研究不同共轭亚油酸异构体对小鼠肌内脂肪沉积和肌肉线粒体代谢的影响.将成长到5周龄的雄性小鼠按体重分成3组(每组8只),适应1周后分别饲喂不添加、添加2％的顺9反11共轭亚油酸(c9,t11-CLA)和添加2％的反10顺12共轭亚油酸(t10,c12-CLA)的日粮,正试期1个月,监测其体重和体组成的变化,饲养试...     

铁与维生素A水平对小鼠子宫分泌蛋白及胚胎存活率影响

探讨铁与维生素A水平对小鼠子宫分泌蛋白及胚胎存活率影响。选取21日龄昆明种小鼠,随机分为9个处理组,每个处理3个重复,饲喂商品日粮2周,随后采用试验饲粮饲喂。试验采用3×3(铁、维生素A)2因子3水平有重复设计(铁添加水平为3.5、35和175 mg/kg,维生素A水平为400、4000和20000 IU/kg),饲喂第11周时,进行交配,此后选有阴栓的小鼠162只,每个处理3个重复,每个重复6只。结果表明:(1)妊娠3 d,与对照组(Fe1-VA1)相比,5组(Fe1-VA2、Fe1-VA3、Fe2-VA2、Fe2-VA3、Fe3-VA1)添加铁与维生素A对胚胎存活率影响显著(P<0.05),分别提高了20.0%、22.7%、24.7%、19.5%和7.7%。妊娠6 d,添加3.5 mg/kg Fe 4000 IU/kgVA、3.5 mg/kg Fe 20000 IU/kg VA、35 mg/kg Fe 4000 IU/kg VA和35 mg/kg Fe 20000 IU/kg VA效果较好(P<0.05)。妊娠18 d,与对照组相比,添加35 mg/kg Fe 4000 IU/kg VA组显著提高了47.4%(P<0.05);(2)铁与维生素A水平组合在对胚胎存活率、酸性磷酸酶含量、视黄醇结合蛋白含量影响显著(P<0.05)。添加35 mg/kg Fe 4000 IU/kg VA可使胚胎存活率、酸性磷酸酶含量、视黄醇结合蛋白含量和子宫总蛋白分泌含量最高;(3)175 mg/kg铁水平组降低了胚胎存活率、酸性磷酸酶、视黄醇结合蛋白和子宫总分泌蛋白含量。35 mg/kg铁水平组使胚胎存活率、酸性磷酸酶含量、视黄醇结合蛋白含量最高,提高了子宫总分泌蛋白含量;(4)4000 IU/kg维生素A组使胚胎存活率、酸性磷酸酶含量、视黄醇结合蛋白含量最大,提高了总分泌蛋白含量;(5)铁对子宫酸性磷酸酶含量影响显著(P<0.05),维生素A对视黄醇结合蛋白含量影响显著(P<0.05)。适量的铁和维生素A有利于胚胎发育;铁与维生素A水平组合对胚胎存活率、酸性磷酸酶含量、视黄醇结合蛋白含量影响存在互作效应。随着铁在3.5~35.0 mg/kg、维生素A在400~4000 IU/kg范围内增加,子宫铁蛋白、视黄醇结合蛋白增加,更好地促进胚胎存活。     

鹿茸口服液对小鼠免疫功能的影响

研究鹿茸口服液(Pilose Antler Oral Liquid,PAOL)对小鼠免疫功能的影响。用环磷酰胺复制免疫抑制小鼠模型,100只昆明种小白鼠随机分成10组,分别为生理盐水对照组、免疫抑制对照组、免疫抑制小鼠4个灌胃剂量组(0.4 mL/d、0.2 mL/d、0.1 mL/d、0.05 mL/d)以及正常小鼠4个灌胃剂量组(0.4 mL/d、0.2mL/d、0.1 mL/d、0.05 mL/d);测定脾脏重量和腹腔巨噬细胞吞噬功能,比色法测定血清溶血素(HC50),MTT法检测植物血凝素(PHA)和脂多糖(LPS)分别诱导的脾脏T、B淋巴细胞转化功能。结果表明,鹿茸口服液0.05 mL可显著增加正常小鼠溶血素水平,其溶血素值达256.067(P<0.01),其中鹿茸口服液0.4 mL、0.2 mL、0.1 mL三个剂量组能够显著提高免疫抑制小鼠溶血素抗体水平(P<0.01);鹿茸口服液0.4 mL、0.1 mL、0.05 mL三个剂量组对正常小鼠T淋巴细胞转化都有较强的促进作用(P<0.01),鹿茸口服液0.1mL剂量组能够显著提高免疫抑制小鼠T淋巴细胞转化功能(P<0.01),0.4 mL剂量鹿茸口服液在促进免疫抑制小鼠B淋巴细胞转化上也体现出明显的效果(P<0.05);其次灌服鹿茸口服液能够增加正常小鼠脾指数,但腹腔巨噬细胞对中性红的吞噬能力相对于对照组没有提高。试验证实,鹿茸口服液能够提高正常小鼠和免疫抑制小鼠机体的免疫功能。     

酵母硒对亚慢性铅中毒小鼠排铅作用的实验研究

研究酵母硒对染铅小鼠排铅作用的影响。选择雄性健康昆明小白鼠90只,随机分为6组,每组15只。1组为阴性对照组,2组为醋酸铅模型组,3组为亚硒酸钠组,4～6组为酵母硒低、中、高剂量组。除阴性对照组外,其余组分别饲喂等量的醋酸铅,30d后测定每组小鼠血液、脑、骨、肝中铅的含量。结果表明,酵母硒组小鼠血液、脑、骨、肝铅水平均显著低于醋酸铅模型组(P<0.05)。可见酵母硒对亚慢性铅中毒小鼠具有明显的排铅作用。