Expression of kinesin superfamily genes in striatum and substantia nigra in MPTP-treated mice

AIM: To investigated the effect of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) on the expression of kinesin superfamily (KIF) genes in striatum and substantia nigra in C57BL mice. METHODS: The Parkinson's disease model was established by consecutive administration of MPTP to C57BL mice. The levels of mRNA for five kinesin superfamily genes, KIF1A, KIF2, KIF3A, KIF4, and KIF5A, in striatum and substantia nigra of mice, were estimated by RT-PCR. RESULTS: In substantia nigra, the expression of KIF genes were decreased after MPTP treatment except KIF2 that showed no significant change. However, the expression of KIF1A, KIF3A and KIF4 were increased in striatum after MPTP treatment, while the expression of KIF2 and KIF5A were similar to that in substantia nigra. CONCLUSION: The lose of dopaminergic neurons in nigrostriatal pathway after MPTP treatment may be related to the expression of KIF genes.     

不同剂型儿茶素对小鼠血清脂质水平的影响

本文探讨了不同剂型儿茶素经不同给予途径对高脂血症小鼠血清脂质水平的影响。结果表明:将儿茶素制成微型胶囊剂型,即经高分子胶体化合物包封后,降低TG、TC、LDL-C和升高HDL-C的作用明显优于游离型儿茶素(P<0.05),静脉注射效果优于口服(P<0.05)。     

Evaluating the immune responses of mice to subcutaneous immunization with Helicobacter pylori urease B subunit

Background： Helicobacter pylori, a gram-negative bacterial pathogen that expresses a strong urease activity, is associated with the development of gastroduodenal disease. Urease B subunit, one of the two structural subunits of urease, was expressed in E. coil BL21 （DE3） strain. The objective of this study was to evaluate the effects of He/icobacter py/ori urease B subunit on the immune responses in mice by subcutaneous immunization. Methods： The mice were immunized and boosted with Helicobacter pylori urease B subunit antigen subcutaneously three times with 2-wk intervals between the immunizations and boosters. The mice in the control group were immunized with PBS. The adjuvant group received PBS containing complete/incomplete freund＇s adjuvant identical to antigen group without Helicobocter pylori urease B subunit antigen. Four weeks after the final booster, all the mice were sacrificed. Blood was collected on d 0, 14, 28 and 56 before immunization, booster and sacrifice, respectively. Immediately after sacrifice, gastric liquid and spleen were collected for antibody and cytokine analyses. Results： Urease B subunit increased the concentrations of serum and gastric anti-urease B antigen specific IgG, and the levels of interteukin-4 and interferon-y in splenocytes of the mice （P 〈 0.05）. Conclusions： This study demonstrated that recombinant responses in mice by subcutaneous immunization, which against Helicobocter pylori. urease B subunit can induce systemic and local immune might be used as the effective component of vaccine     

不同剂量速效救心丸对妊娠小鼠血液指标的影响

通过在饲料中拌饲不同剂量的速效救心丸,测定其对妊娠小白鼠血液指标的影响,探讨速效救心丸对缓解妊娠小鼠胎儿宫内生长迟缓(intrauterine growth retardation,IUGR)的作用.将140只雌鼠随机分为对照组、250 mg/kg、500 mg/kg和750 mg/kg速效救心丸组.待小白鼠妊娠15 ...     

Establishment of an acute GVHD animal model in EL9611 erythroleukemia mice

AIM: To establish an acute graft-versus-host disease (GVHD) model in EL9611 erythroleukemia mice. METHODS: Using C57BL/6 (H-2^b) mice as the donor and BALB/c (H-2^d) mice as the recipient in allogeneic bone marrow transplantation (allo-BMT), the acute GVHD model was established. The mice were divided into leukemia group (n=10), radiation control group (leukemic mice given radiation without allo-BMT, n=4), GVHD group (leukemic mice given radiation+allo-BMT, n=10) and normal control group (n=4). In leukemia group, 2×10⁶/mouse EL9611 erythroleukemic cells were transfused via tail vein into BALB/c mice to build the erythroleukemia model. In GVHD group, 7 days after leukemic cell transfusion, the mice received total dose of 8.0 Gy γ of total body irradiation(TBI), and within 5 h, 2×10⁶ C57BL/6 bone marrow cells and 1×10⁷ C57BL/6 spleen cells per mouse were transfused via tail vein to build the acute GVHD model in EL9611 erythroleukemia mice. The clinical manifestations of posture, fur, stool and so on were observed. Pathological examination was conducted to examine the changes of liver, spleen, skin, small intestine and peripheral blood. The survival rate was also calculated. RESULTS: (1) In leukemia group, the mean survival time (MST) was (14.5±2.1) days,or (7.5±0.7) days when irradiation day was as day 0(P<0.01 compared with GVHD group). The death rate was 100% with no spontaneous remission. The dead mice showed splenohepatomegalia and high WBC count . Pathological examination showed disorganization of normal tissues and leukemic cell infiltration. (2) In radiation control group, MST was (9.0±0.7) d, with significant difference as compared with GVHD group and normal group (P<0.01). The death rate was 100%. Pathological examination showed hematopoiesis exhaustion. (3) In GVHD group, MST was (32.0±3.2) d (P<0.01 compared with other groups). The death rate was 100%, the symptoms were observed on day 10-13 after allo-BMT. Clinical manifestations and pathological examination corresponded to those of I degree to II degree of GVHD. CONCLUSION: Intravenous infusion of 2×10⁶/mouse EL9611 leukemic cell successfully establishes the EL9611 erythroleukemia animal model. Seven days after EL9611 leukemic cell transfusion, lethal dose of TBI and allo-BMT can successfully build the acute GVHD model of EL9611 leukemic mice.     

华蟾毒精对小鼠免疫细胞活性的影响

为观察华蟾毒精（CBG）对小鼠免疫细胞活性的影响,本研究采用MTT法检测小鼠脾淋巴细胞的增殖情况,检测小鼠腹腔巨噬细胞的吞噬功能以及小鼠NK细胞对靶细胞的杀伤作用。结果显示,CBG在一定剂量范围内单独或者协同非特异性丝裂原（Con A或LPS）作用能够显著增强小鼠脾淋巴细胞的增殖,CBG单独作用可以显著提高小鼠腹腔巨噬细胞的吞噬功能,并能够显著提高小鼠NK细胞对靶细胞的杀伤作用,表明CBG能够提高小鼠免疫细胞的活性。     

Phenotype evaluation of human and canine isolates of Leishmania infantum

Human visceral leishmaniasis (VL) and canine leishmaniasis (CanL) in countries of South and Central America are caused by Leishmania infantum and has been endemic in Brazil for several years. The parasite biodiversity as well as the pharmacologic properties of drugs and the host species, are involved in the efficacy or inefficacy of leishmaniasis treatments. Although there are substantial number of reports describing the genetic characterization of the clinical field isolates of L. infantum,the phenotypic parameters have been less studied. In this study isolates from human and canine leishmaniasis (Hum1 and Can1) obtained in Campinas, São Paulo state, Brazil were identified as L. infantum. The Hum1 and Can1 isolates exhibited typical promastigote growth pattern. Regarding morphological features Can1 isolate differed in cell size. The infectivity in vitro of both isolatesis lower compared to the reference strain of L. infantum. Moreover, the in vivo infectivity of the three parasites is similar in Balb/c mice. The Hum1 isolate is more sensitive to leishmanial drugs (amphotericin B, miltefosine and glucantime) than the Can1 isolate when inside human macrophages, but not when inside canine macrophages. These findings indicated that L. infantum isolates differs in some phenotypic characteristics.     

鹿茸提取物对糖尿病小鼠血糖及衰老的影响

为了研究鹿茸提取物对四氧嘧啶(ALX)诱导糖尿病小鼠的降糖和抗衰老作用,利用ALX建立了糖尿病小鼠模型,并将鹿茸提取物(RD)分为低(1.6mg/g)、中(3.2mg/g)、高(4.8 mg/g)3个剂量组,对建模成功小鼠连续灌胃4周.试验结果与模型对照组相比,鹿茸提取物能极显著地降低ALX糖尿病小鼠血糖水平、丙二醛(MDA)浓度,提高肝(肌)糖元质量分数以及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、麦芽糖酶(CAT)的活性,增强机体抗氧化能力.鹿茸提取物能有效地控制糖尿病小鼠血糖水平,提高机体抗衰老能力.     

克林霉素对雄性小鼠精子功能的影响

[目的]研究克林霉素对雄性小鼠生殖细胞的影响。[方法]取48只健康成年雄性小白鼠,随机分为试验A组、B组、C组和对照组,每组12只小鼠。试验A、B和C组分别注射克林霉素15、30和60 mg/kg.d,对照组腹腔注射生理盐水。29 d后,脱臼处死小鼠,观察小鼠精子的活率、畸形率、精子膜低渗肿胀率。[结果]试验B、C组小鼠精子活率和尾膜肿胀比率低于对照组,而畸形率高于对照组;试验A组小鼠精子活率、畸形率以及尾膜肿胀比率与对照组相比无显著差异。[结论]克林霉素使用剂量等于或超过重度感染使用剂量时会影响雄性小鼠精子的正常功能。     

蒲公英水提液对小鼠抗疲劳作用的研究

[目的]研究蒲公英水提液对小鼠抗疲劳作用。[方法]通过试验测定其运动后血乳酸、血糖、肌糖原、肝糖原、血清尿素氮的含量以及游泳力竭时间等指标。[结果]与对照组相比,试验组小鼠,除血糖变化不明显,肌糖原、肝糖原含量均显著增加（P〈0.05）,血乳酸及血清尿素氮水平均明显降低（P〈0.05或P〈0.01）,游泳力竭时间显著延长（P〈0.01）。[结论]蒲公英水提液具有明显抗疲劳作用。