“畜禽用多种微量元素注射液”对小鼠免疫功能的影响

将60只昆明小鼠(体质量为18~22 g,雌雄各半)随机分为3组,每组20只。再将每组的20只分成试验组和对照组,每组10只,试验组每天腹腔注射0.04 mL"多种微量元素注射液",对照组注射0.04 mL生理盐水,连续注射7 d。3组分别进行酸性α-醋酸萘脂酶法试验,溶血空斑试验和血清溶血素抗体(HC50)测定。结果表明,试验组小鼠ANAE阳性细胞百分比为51.80%±3.645%,极显著高于对照组的39.75%±4.283%(P0.01);试验组小鼠溶血空斑形成数量为23.1±2.13177,极显著高于对照组的17.4±2.01108(P0.01);试验组小鼠血清溶血素水平为262.2900±10.48257,显著高于对照组的231.2710±39.61145(P0.05)。即"畜禽用多种微量元素注射液"能显著提高小鼠的免疫功能。     

青钱柳醇提取物对糖尿病小鼠降血糖作用的研究

采用四氧嘧啶选择性破坏昆明种小鼠的胰岛β细胞,造成实验性糖尿病模型小鼠共160只,随机分成5组,每组2个重复,设消渴丸组、四氧嘧啶组,青钱柳提取物高、中、低浓度组。尔后用青钱柳醇提取物给该小鼠灌胃,测定其空腹血糖浓度,观察小鼠血糖浓度和体重变化,最后进行糖耐量试验。结果表明:在灌胃青钱柳70%醇提取物2周和3周后,高、中浓度的青钱柳醇提取物组小鼠的血糖浓度明显低于糖尿病模型组小鼠的血糖(P<0 05);给药4周后,高、中浓度组和消渴丸组小鼠的血糖浓度明显低于糖尿病模型组的血糖浓度(P<0 05);给药5周和6周后,低浓度组和消渴丸组小鼠的血糖浓度明显低于糖尿病模型组的血糖浓度(P<0 05),而高、中浓度组小鼠的血糖浓度与糖尿病模型组的血糖浓度相比,差异极显著(P<0 01);在第4周和第5周时,低浓度组小鼠的体重明显低于空白对照组小鼠的体重(P<0 05);同时,青钱柳对试验性小鼠的糖耐量具有显著的增强作用。     

黄芩素对感染鼠伤寒沙门氏菌小鼠的影响

试验研究黄芩素对感染鼠伤寒沙门氏菌(STM)小鼠的影响.将80只小鼠分为空白组、STM组、黄芩素+STM组和黄芩素组.结果 显示:STM使感染小鼠的存活率明显下降,小鼠血清中炎性因子水平明显增高,脏器指数降低,肝脏和脾脏组织呈现明显的病理改变.黄芩素可使感染STM小鼠的存活率明显升高,小鼠血清中炎性因子水平降低,脏器指...     

蛋清小肽混合物对两种免疫状态小鼠生产性能、血清生化指标及生长激素水平的影响

本试验旨在研究不同剂量蛋清小肽混和物(MSEE)对两种免疫状态小鼠生产性能及血清生化指标和生长激素(GH)水平的影响.试验选用120只体重(15±2)g(约5～6周龄)普通级昆明种雄性小白鼠随机分成2组(正常组和环磷酰胺诱导的免疫功能低下组),每组设3个水平(每千克体重分别腹腔注射蛋清小肽混合物0、50和 200 mg),每个水平4个重复,每个重复5只小鼠.各组试验日粮相同,试验期1周.结果表明:(1)与正常组相比,免疫功能低下组平均日增重(ADG)、平均日采食量(ADFI)及血清总蛋白(TP)、白蛋白(Alb)、球蛋白(Glob)和GH水平显著(P<0.05)或极显著(P<0.01)降低,料重比(F/G)极显著提高(P<0.01),(2)MSEE处理后,两种免疫状态小鼠间的各项检测指标均无显著差异(P>0.05),(3)MSEE可提高小鼠尤其是免疫功能低下小鼠的生产性能 和血清GH水平,并表现出相似地剂量效应关系,同时还可改善血清生化指标.本研究揭示,MSEE可有效地促进小鼠尤其是免疫功能低下小鼠生产潜能的发挥.     

The effects of deltamethrin on some serum biochemical parameters in mice

Sixty white male mice were used in this study. Three groups, each comprising 20 mice were established. The control group (Group 1) was provided ad libitum pellet feed. On the other hand, the experimental groups, namely, Groups 2 and 3 were given pellet feed containing deltamethrin throughout the day, so that the animals were administered doses of 7.5 and 30 mg/kg/body weight/day, respectively. Blood samples were collected from all groups on the 15th, 30th, 45th, and 60th days of the experiment for measurement of serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alcaline phosphatase (ALP), amylase and cholinesterase activities, and levels of glucose, urea, creatinine, triglyceride, cholesterol, calcium, phosphor, sodium, potassium, and chloride. According to data obtained, on the 15th day of the study, compared to the control group, statistically significant increase in urea, cholesterol, ALP, cholinesterase, calcium, and potassium levels/activities, and significant decrease in SGOT activity and chloride levels in Group 2, and significant increase in cholesterol, ALP, cholinesterase, calcium, potassium and chloride levels/activities in Group 3, and significant decrease in SGOT activity in Group 3 were determined. On the 30th day of the study, in comparison to the control group, triglyceride and cholesterol levels and ALP activity were determined to be significantly increased in the Group 2, whereas SGOT activity were significantly reduced in Group 2, and triglyceride and cholesterol levels were demonstrated to be significantly increased and SGOT activity was significantly reduced in Group 3. On the 45th day of the study, compared to the controls, significant increase in cholesterol and sodium levels, and significant decrease in triglyceride levels and ALP activity in Group 2, significant increase in creatinine, cholesterol and sodium levels and cholinesterase activity, and significant decrease in glucose, SGPT, ALP and phosphor levels/activities were detected in Group 3. On the 60th day, in other words, in the last period of the study, ALP activity, and triglyceride, calcium, and phosphor levels were determined to be significantly increased and significant decrease in amylase activity in Group 2. Furthermore, significant increase in triglyceride, ALP, amylase, calcium, phosphor, and potassium levels/activities, and significant decrease in glucose and creatinine levels were observed in Group 3. However no correlation was determined to exist between the changes were found to be statistically significant, and the administration dose and duration of deltamethrin in all periods and groups.     

Study on efficacy of the decoction of invigorating kidney and activating blood circulation in BALB/C mice with immune mediated aplastic anemia

AIM and METHODS: To study the efficacy of the decoction of invigorating kidney and activating blood circulation(DIKABC) in BALB/C mice with immune mediated aplastic anemia. 50 female BALB/C mice at 8~12 weeks of age were divided into 5 groups:(1) control,(2) model,(3) model+DIKABC(H), (4) model+DIKABC(L), and (5) model+ciclosporin A(CsA). The model of immune mediated aplastic anemia in BALB/C mice, which received sublethal whole body irradiation, lymph node and thymus cells of DBA/2 mice, was given different liquid from groups and administered appropriately for 9 days, blood cells and bone marrow nucleated cells(BMNC) and CD₄₅⁺ cells were determined by flow cytomytry(FCM).RESULTS: RBC,WBC,Platelet,BMNC in DIKABC groups were higher than those in model group (P＜0.01/0.05), but those in CsA group were lower than those in DIKABC groups (P＜0.05).Compared with model group, CD₄₅⁺ cells in DIKABC groups increased (P＜0.05),but were lower than those in control groups (P＜0.05). CONCLUSION: The decoction of invigorating kidney and activating blood circulation could increase the percentages of CD₄₅⁺ cells in bone marrow and prevent immune mediated aplastic anemia in BALB/C mice.     

铁与维生素A水平对小鼠子宫分泌蛋白及胚胎存活率影响

探讨铁与维生素A水平对小鼠子宫分泌蛋白及胚胎存活率影响。选取21日龄昆明种小鼠,随机分为9个处理组,每个处理3个重复,饲喂商品日粮2周,随后采用试验饲粮饲喂。试验采用3×3(铁、维生素A)2因子3水平有重复设计(铁添加水平为3.5、35和175 mg/kg,维生素A水平为400、4000和20000 IU/kg),饲喂第11周时,进行交配,此后选有阴栓的小鼠162只,每个处理3个重复,每个重复6只。结果表明:(1)妊娠3 d,与对照组(Fe1-VA1)相比,5组(Fe1-VA2、Fe1-VA3、Fe2-VA2、Fe2-VA3、Fe3-VA1)添加铁与维生素A对胚胎存活率影响显著(P<0.05),分别提高了20.0%、22.7%、24.7%、19.5%和7.7%。妊娠6 d,添加3.5 mg/kg Fe 4000 IU/kgVA、3.5 mg/kg Fe 20000 IU/kg VA、35 mg/kg Fe 4000 IU/kg VA和35 mg/kg Fe 20000 IU/kg VA效果较好(P<0.05)。妊娠18 d,与对照组相比,添加35 mg/kg Fe 4000 IU/kg VA组显著提高了47.4%(P<0.05);(2)铁与维生素A水平组合在对胚胎存活率、酸性磷酸酶含量、视黄醇结合蛋白含量影响显著(P<0.05)。添加35 mg/kg Fe 4000 IU/kg VA可使胚胎存活率、酸性磷酸酶含量、视黄醇结合蛋白含量和子宫总蛋白分泌含量最高;(3)175 mg/kg铁水平组降低了胚胎存活率、酸性磷酸酶、视黄醇结合蛋白和子宫总分泌蛋白含量。35 mg/kg铁水平组使胚胎存活率、酸性磷酸酶含量、视黄醇结合蛋白含量最高,提高了子宫总分泌蛋白含量;(4)4000 IU/kg维生素A组使胚胎存活率、酸性磷酸酶含量、视黄醇结合蛋白含量最大,提高了总分泌蛋白含量;(5)铁对子宫酸性磷酸酶含量影响显著(P<0.05),维生素A对视黄醇结合蛋白含量影响显著(P<0.05)。适量的铁和维生素A有利于胚胎发育;铁与维生素A水平组合对胚胎存活率、酸性磷酸酶含量、视黄醇结合蛋白含量影响存在互作效应。随着铁在3.5~35.0 mg/kg、维生素A在400~4000 IU/kg范围内增加,子宫铁蛋白、视黄醇结合蛋白增加,更好地促进胚胎存活。     

纳米氧化铜对小白鼠血浆抗氧化机能的影响

选144只20g左右的健康昆明种小白鼠,随机分为6组,每组设4个重复。1组饲喂基础日粮;2组在基础日粮中以硫酸铜的形式添加铜10mg/kg;3组在基础日粮中以氧化铜的形式添加铜10mg/kg;4～6组在基础日粮中以纳米氧化铜的形式分别添加铜5、10、20mg/kg。研究不同剂量纳米氧化铜对小白鼠血浆抗氧化能力的影响,试验期30d。结果表明,添加纳米氧化铜显著提高小白鼠血浆铜蓝蛋白活力(P<0.05),显著提高小白鼠血浆细胞色素C氧化酶活性(P<0.05),显著降低30d小白鼠血浆丙二醛含量(P<0.05)。     

Inhibitory effect of liposomes survivin antisense oligonucleotide on human hepatic carcinoma transplanted subcutaneously in nude mice

AIM: To explore the effects of liposomes survivin antisense oligonucleotides (ASODN) on growth of human hepatic carcinoma transplanted subcutaneously in nude mice. METHODS: Nude mouse model of human hepatic cancer was established by transplantation of hepatic cancer cell line SMMC-7721/ADM subcutaneously. Models were divided randomly into six groups: control group, liposome group, sense oligonucleotide (SODN) group, 200 μg/L, 400 μg/L and 600 μg/L ASODN groups. Different treatments were given respectively. Weight and volume of subcutaneous tumors were measured, and tumor growth inhibitory rate was calculated. Morphological changes of transplanted tumor cells were observed under light microscope. The expression of Survivin was detected by immunohistochemistry. RESULTS: The growth of tumors was significantly inhibits in all ASODN groups compared with control, liposome and SODN groups (P<0.05). Volume of subcutaneous tumors decreased in a time-dependent and dosage-dependent manner (P<0.5). CONCLUSION: Survivin ASODN inhibits the growth of human hepatic carcinoma in nude mice.     

桑椹素对氢化可的松致老龄阳虚模型小鼠的影响

桑椹素是以桑椹为主要成分的保健药品。将ICR雄性老龄小鼠随机分为正常组、模型组,以及桑椹素低、中、高剂量组(0.1,0.2,0.4 g/kg)和补肾填精丸组(0.35 g/kg),除正常组外各组均肌注氢化可的松造模,21 d后测定小鼠肝组织三磷酸原苷酶(Na+-K+-ATPase,Ca2+-Mg2+-ATPase)、超氧化物歧化酶(SOD)活性,以及还原型谷胱甘肽(GSH)、丙二醛(MDA)、丙酮酸、甘油三酯(TG)含量和血清SOD、GSH、MDA的水平变化情况,同时通过光镜观察小鼠肝脏组织结构病理变化。结果表明,以0.2、0.4 g/kg剂量桑椹素灌胃可显著提高模型小鼠肝组织ATP酶活性和降低丙酮酸、TG含量,还可提高抗氧化剂SOD、GSH水平,降低脂质过氧化物MDA含量,减轻氢化可的松引起的肝细胞浊肿、脂肪变性等病理变化。桑椹素对老龄阳虚模型小鼠肝脏具有明显的保护作用,其作用机制可能是通过促进糖、脂的氧化代谢提供充足的ATP,提高肝组织ATP酶活性;同时还可提高阳虚模型小鼠血清和肝脏中的SOD、GSH水平,清除体内自由基,以减轻其对肝细胞的损害,降低能量代谢障碍。