Effects of dietary tryptophan and stocking density on the performance,meat quality,and metabolic status of broilers

Background

Highly automated cage-rearing systems are becoming increasingly popular in China. However, a high stocking density can cause oxidative stress and decrease broiler performance. The tryptophan (TRP) derivative 5-hydroxytryptophan (5-HT) has been shown to preserve membrane fluidity in birds suffering from oxidative stress. Therefore, this experiment was conducted to determine the effects of dietary TRP supplementation on performance, breast meat quality and oxidative stress in broilers reared in cages with a high or low stocking density.

Methods

Female Arbor Acres broilers (25-d-old, n = 144) were randomly allocated to 1 of 4 treatments. The birds were fed a diet based on corn, soybean meal, cottonseed meal and corn gluten meal containing either 0.18 or 0.27% TRP and were housed with stocking densities of 11 or 15.4 birds/m² in a 2 × 2 factorial experiment. Broiler performance was evaluated from d 25 to 42. Eight birds from each treatment were slaughtered on d 42 and plasma and breast muscle samples were collected to measure biochemical indices.

Results

A higher stocking density tended to be associated with reduced weight gain (P < 0.10), and significantly increased plasma glutamic-pyruvic transaminase (GPT) activity (P < 0.001). Increased dietary TRP significantly reduced the activities of lactic dehydrogenase and GPT while increasing total cholesterol in the plasma (P < 0.01), reducing drip loss of breast muscle (P < 0.10) and improving feed efficiency (P < 0.10).

Conclusions

An increase in dietary TRP, 1.5-fold higher than the standard supplementation level, can alleviate oxidative stress as well as improve welfare and feed efficiency in broilers reared in cages with a high stocking density.     

饲料中添加油脂对肉鸭生产性能的影响

本试验选用1日龄樱桃谷肉鸭500只,随机分为10个组(9个试验组和1个对照组),每组50只肉仔鸭。试验组采用二因素、每个因素三个水平的完全随机设计,即在饲粮中添加3%、6%、和9%的大豆油,每个比例分别添饲1周、2周、3周,旨在研究日粮中添加大豆油的用量和时间对肉鸭生产性能的影响。结果表明:各试验组之间以及各试验组与对照组之间肉鸭的死亡率无显著差异(P>0.05);各试验组与对照组之间肉鸭的饲料转化率有显著差异(P<0.05),各试验组之间肉鸭的饲料转化率无显著差异(P>0.05);添加大豆油的比例、时期及比例与添饲时的互作对肉鸭的日增重均有极显著的影响(P<0.01),而以添加6%的比例添饲两周的效果最好。     

反复冻融对肉制品品质的影响

冷冻肉制品在现代肉及肉制品加工工业中起着很重要的作用,是原料肉最常用的保存方法。储存和运输时温度的波动,以及一些销售环节温度控制不当,会引起肉制品反复冷冻-解冻(反复冻融),这会引起肉制品内部的一系列生理生化反应,进而导致肉制品品质的下降。就反复冻融对肉制品品质方面的影响展开论述,以期为原料肉的合理冷冻储存提供参考。     

维生素A对獭兔血液生化指标的影响

为了明确维生素A的合理用量,以90只獭兔为研究对象,研究了维生素A对獭兔血液生化指标的影响。结果表明,在基础日粮中添加10 000IU·kg-1维生素A对獭兔血液ALB和BUN含量有显著影响,ALB比对照提高了10.35%,BUN比对照下降了5.30%。因此,建议獭兔日粮中维生素A的添加量为10 000IU·kg-1。     

优良牧草在家兔生产中的研究进展

优良牧草不仅蛋白质含量高,适口性好,且含有丰富的矿物质元素、胡萝卜素以及多种维生素等,是家兔优良高效生产重要的饲料。文章对优良牧草在家兔生产中的应用作以综述。     

土霉素固体脂质纳米粒在家兔体内的药代动力学研究

【目的】研究土霉素固体脂质纳米粒在家兔体内的药代动力学特征,以评价其应用效果。【方法】将10只家兔随机分为2组,分别按土霉素剂量20mg/kg腹腔注射土霉素固体脂质纳米粒和土霉素注射液,于设定的时间点采血,用HPLC法测定血浆药物质量浓度,并用DAS3.1.0药动学程序计算药代动力学参数。【结果】土霉素注射液与固体脂质纳米粒的达峰质量浓度(Cmax)分别为(3.676±0.376)和(3.699±0.562)mg/L;吸收平衰期(T1/2Ka)分别为(18.570±0.851)和(27.871±1.767)h;0-∞时段药时曲线下的面积(AUC(0-∞))分别为(83.955±3.720)和(170.150±10.403)mg/(L·h);0-∞时段的平均滞留时间(MRT(0-∞))分别为(36.848±0.268)和(44.672±0.392)h。【结论】土霉素制备成固体脂质纳米粒后,具有明显的缓释作用,血药质量浓度达峰值的时间推迟,同时药物在兔体内平均滞留时间明显长于土霉素注射液,生物利用度得以显著提高。     

双乙酸钠在肉鸭日粮中的添饲效果试验

选用1日龄健康樱桃谷肉鸭600只,随机分为3组,每组设4个重复,每个重复50只肉鸭,试验采用单因素完全随机试验设计。A组饲喂基础日粮,B组、C组在基础日粮的基础上分别添加0.2%、0.4%的双乙酸钠。试验结果表明:日粮中添加双乙酸钠对肉鸭日增重、瘦肉率有显著影响(P<0.05);对肉鸭的饲料转化率有极显著影响(P<0.01);对肉鸭的成活率和屠宰率、皮脂率无显著影响(P>0.05)。B组日增重比A组提高了8.65%(P<0.05),比C组提高了5.45%(P>0.05);B组料肉比较A组降低了11.52%(P<0.01),较C组降低了3.29%(P<0.05);B组的成活率与A组、C组间差异不显著(P>0.05);B组每只肉鸭分别比A、C组多盈利1.04元、1.06元。     

丝羽乌骨鸡快大型新品系早期生长发育规律及肉用性能的研究

本试验对丝羽乌骨鸡快大型新品系的早期生长发育规律及肉用性能进行了系统的研究。利用Logistic模型确定了快大型新品系丝羽乌骨鸡的生长模型,并以此制定了标准生长曲线。研究结果表明,其生长拐点为:10.5周龄;在14周龄时体重约1.0kg,公、母鸡屠宰率分别达到85.88%、84.12%;胸肌率分别达到17.78%、17.28%;腿肌率分别达到24.38%、22.54%。在14周龄时,为较适合的上市日龄。     

Effects of Dietary Lysine on Growth Performance,Serum Concentrations of Insulin-Like Growth Factor-I （IGF-I） and IGF-I mRNA Expression in Growing Rabbits

The effects of dietary lysine on production performance,serum concentrations of metabolites,growth hormone （GH）,insulin-like growth factor-I （IGF-I） and IGF-I mRNA expression in growing rabbits were examined.One hundred weaned New Zealand rabbits were allocated to individual cages and randomly offered a diet containing 5.5 （L1）,6.5 （L2）,7.5 （L3）,8.5 （L4）,or 9.5 g （L5） lysine per kg diet.The results showed that the average daily gain （ADG） of the rabbits from L3,L4 or L5 was higher than those from L1 or L2 （P 〈 0.05）.The feed gain ratio （F/G） in the rabbits from L4 or L5 was lower than those from L1 or L2 （P 〈 0.05）.Dietary lysine did not affect serum concentrations of total protein （TP）,glucose,insulin （INS）,and growth hormone （GH） （P 〉 0.05）.The quadratic effects of lysine on the serum urea nitrogen （SUN） concentration was detected （P = 0.035）.Serum IGF-I concentrations had a trend to increase quadratically with the increasing dietary lysine （P = 0.07）.A significant correlation was found between serum IGF-I concentrations （x,ng mL-1） and ADG （y,g kg-1）： y = -0.017x2 ＋ 1.984x ＋ 20.87 （R2 = 0.8982,P = 0.003）.The relative abundance of hepatic and muscular IGF-I mRNA tended to increase with increasing dietary lysine levels （P = 0.053 and 0.082,respectively）.Providing the diets mainly consisted of corn,wheat bran and peanut vine,the most appropriate dietary lysine level for growing meat rabbits from weaning to 70 d old was found to be 8.5 g kg-1,and IGF-I may be an important factor controlling growth of weaned rabbits.     

Effects of dietary supplementation with Allium mongolicum Regel extracts on growth performance,serum metabolites,immune responses,antioxidant status,and meat quality of lambs

The present study was to evaluate the effects of dried Allium mongolicum Regel (AMR) powder and its water- and fat-soluble extracts (AWE and AFE) on the growth performance, serum metabolites, immune responses, antioxidant status, and meat quality of lambs. A total of 32 male small-tailed Han lambs (5 months old; initial body weight = 34.8 ± 0.40 kg) were used in a 60-d feeding experiment after a 15-d adaptation period. The lambs were randomly divided into 4 groups (n = 8) and fed a basal diet (control, CON group), the basal diet supplemented with dried AMR powder at 10 g/d per lamb (AMR group), the basal diet supplemented with AWE at 3.4 g/d per lamb (AWE group), or the basal diet supplemented with AFE at 2.8 g/d per lamb (AFE group). Blood samples were collected on d 0, 30, and 60 in the feeding experiment (n = 8). At the end of the experiment, the lambs were sacrificed and the longissimus dorsi muscles collected. Growth performance was not significantly affected by dietary supplementation of AMR, AWE and AFE (P > 0.05). However, significantly lower albumin (P = 0.006), total protein (P = 0.006), globin (P = 0.025), and blood urea nitrogen (P = 0.024) concentrations were observed in AFE group relative to CON and AMR groups. Similarly, a significantly lower lactate dehydrogenase activity (P = 0.018) was observed in AFE group relative to AWE group, but not in other groups (P > 0.05). In addition, significantly increasing trends in glutathione peroxidase (P = 0.06) in AMR, AWE, and AFE groups were observed relative to the control group. Furthermore, significantly lower drip loss (P = 0.011) across the treatment groups and cooking loss (P = 0.048) were observed in the AMR group relative to the control group. Taken together, these results indicate that AMR and its extracts had no significant effect on lamb growth performance, antioxidant status, and immune responses, but could significantly improve meat quality without the occurrence of pathological kidney and liver lesions.