不同温度下雌雄葎草营养生长期的生长特性

通过测定15、20、25 ℃培养条件下,雌雄葎草(Humulus scandens)单株营养生长期持续时间,根、茎、叶等构件数量性状及鲜质量、干质量和生物量分配等指标,分析温度对营养生长期雌雄葎草生长特性的影响。结果表明,温度对葎草营养生长持续期有极显著影响（P＜0.01）,雌株比雄株更易受影响;温度极显著影响根、茎、叶等构件数量性状及其鲜质量与干质量;温度极显著影响营养生长期雌、雄葎草单株的生物量总量及构成,雄株通过减少叶生物量、雌株通过增加根系生物量来为生殖生长提供物质基础。     

桔皮提取物抑制霉菌试验

试验选用乙醚,丙酮,乙醇３种溶剂提取桔皮中有效成分,分别制成６０％的乳剂,用灭菌蒸馏水稀释成不同浓度梯度,加入培养其中进行抑制霉菌试验。     

棘托竹荪的生物学特征

棘托竹荪的生物学特征周崇莲（中国科学院沈阳应用生态研究所沈阳１１００１５）曾德容（湖南合同县政协会同４１８３００）关键词棘托竹荪，形态特征，生态习性，营养成份１９８５年对湖南会同县的竹荪进行野外调查时，采到一种不同于竹荪（Ｄｉｃｔｙｏｐｈｏｒａｉｎｄ...     

The impact of Theileria parva infections and other factors on calf mean daily weight gains in smallholder dairy farms in Murang'a District, Kenya

The association between mean daily weight gain, Theileria parva infections, clinical East Coast fever and other possible determinants of weight gain were examined in a longitudinal observational study that was conducted in cohorts of female calves from five agro-ecological zone (AEZ)-grazing strata. The strata were upper-midlands (UM) 1 zero-grazing, UM 1 open-grazing, UM 2 zero-grazing, UM 4 zero-grazing and UM 4 open-grazing. In total, 225 calves on 188 smallholder dairy farms were visited within the first 2 weeks of life and thereafter at biweekly intervals up to the age of 6 months between March 1995 and August 1996. During each visit, the calves were weighed and other calf-management practices in the farm during the visit such as housing, feeding and tick control also were recorded. Other events such as morbidity and mortality between or during the visits were also recorded.

The overall mean daily weight gains were 0.24–0.29 kg (S.D.=0.17–0.22 kg) and were lower than the recommended targets for smallholder farms of 0.40–0.50 kg. The major tendency in variability of daily weight gains was due to visit-to-visit variation (especially in calves >3 months old).

Differences in mean daily gains were associated with AEZ-grazing strata and calf-level factors that included breed of calf, calf sickness, incidence of ECF, feeding of milk, concentrate feeds and minerals and interaction between calf age and AEZ-grazing strata (P<0.05). ECF and other calf sicknesses exerted a temporal effect on calf-growth at the height of illness and immediately after; calves later recovered the lost growth except where other factors such as poor calf nutrition prevailed. Improvement in calf-growth in Murang’a District is achievable and extension services should continue to target individual-calf-level management practices.     

高原鼢鼠肌肉脂溶性物质抗缺氧机制的探讨

为了探讨高原鼢鼠肌肉脂溶性物质（FSC）抗缺氧机制。分别以10％FSC、5％FSC、1％FSC、0.5%CLA(阳性对照）和蒸馏水（阴性对照）给小鼠灌胃，连续10d，在常压缺氧条件下测定小鼠存活时间，并测定小鼠心肌和骨胳肌肌红蛋白含量。结果显示，在浓度为10％时，FSC能显著提高小鼠在缺氧条件下的生存时间，并显著提高小鼠心肌和骨胳肌肌红蛋白含量。     

施氮水平对青海中华羊茅产量和营养成分含量的影响

在高寒地区旱作条件下种植青海中华羊茅最适施氮量为N60kg/hm^2,施肥后三年内产草量比对照提高23.62%,其增产值是施肥成本的2.37倍,产籽量提高12.38%,其增产值是施肥成本的4.97倍。青海中华羊茅的产量随着施氮量的增加而增加,施氮后第二年的增产量高于施肥当年的增产量。粗蛋白质含量随着施氮量的增加而增加,但其增加速度随着施氮量的增加而减少,无氮浸出物的含量则随着施氮量的增加而急剧减少,而减少的速度随着施氮量的增加而减小。     

九龙牦牛体型线性性状研究

对24头九龙牦公牛和252头牦母牛的9个体型线性性状进行主成份和因子分析，牦公牛被提取3个因子，累积方差贡献率达86.407％，分别是：体型综合信息因子、胸宽因子和十字部高－－腰角宽因子；牦母牛被提取5个因子，累积方差贡献率达81.93%，分别是：高度因子、后躯－－长度因子、胸部特征因子、管围因子和胸宽－－尻斜长因子。牦公牛3个因子表达式分值、牦母牛5个因子表达式分值，用于牦牛体型评估，具有较高的选育和经济价值。     

Expression of PDGFR-β and Kit in canine anal sac apocrine gland adenocarcinoma using tissue immunohistochemistry

Canine anal sac apocrine gland adenocarcinoma (ASAGAC) is an uncommon but highly invasive and metastatic malignancy. Toceranib phosphate (Palladia) is a receptor tyrosine kinase (RTK) inhibitor that targets several members of the split kinase RTK family. These membrane receptors are important for cell cycling, apoptosis and angiogenesis, all of which can contribute to carcinogenesis. The objective of this study was to evaluate archived, paraffin-embedded canine ASAGAC and normal canine anal sacs for immunohistochemical detection of Kit and platelet-derived growth factor receptor beta (PDGFR-β). Two of 77 neoplasms (2.6%) expressed Kit. Fifteen of the neoplasms (19.5%) were positive for PDGFR-β expression. None of the normal canine anal sac epithelium expressed Kit or PDGFR-β. Because of these results, further investigation should be considered to determine the role of RTKs in the clinical course and treatment of canine ASAGAC.     

113份饲草型六倍体小黑麦种质饲草产量与品质性状的评价

种质资源是遗传研究与作物改良的基础。饲草产量与品质是决定饲草型小黑麦品种利用价值的重要指标。本研究以113份国内外小黑麦种质为材料,通过2年田间种植,对其饲草产量与品质性状进行了分析与评价。结果表明参试小黑麦种质的饲草产量及其品质性状存在极显著差异,其单株鲜草产量分别为36.000～111.560 g与36.310～159.780 g,单株干草产量分别为12.000～27.000 g与9.150～30.150 g,鲜干比分别为2.380～4.370与2.610～6.210,饲草粗蛋白含量分别为6.894%～13.259%与6.680%～14.304%,饲草中性洗涤纤维含量分别为48.480%～74.850%与53.850%～67.980%,酸性洗涤纤维含量分别为26.600%～42.780%与29.000%～39.280%,饲草的相对饲用价值分别为69.650～128.150与79.840～113.170。饲草产量与品质性状的多样性指数范围为1.974～2.075。主成分分析表明,饲草纤维品质因子、饲草产量因子与综合品质因子为前3个主成分,其累计贡献率为82.198%。依据单株干草产量...     

基于表面等离子共振技术鉴定鸡补体受体2与其配体chC3 d的亲和力

鸡补体受体2(Chicken complement receptor 2, chCR2)是一种表达于鸡B淋巴细胞表面的跨膜蛋白,chCR2可以与其生理学配体鸡补体组分3 d(Chicken complement component 3 d, chC3 d)相互作用。目前评估受体与配体亲和力的方法主要有表面等离子共振技术(Surface plasmon resonance, SPR)和生物膜干涉技术(Biolayer interferometry, BLI)等。旨在利用表面等离子共振技术鉴定chCR2受体分子与其配体chC3 d分子的亲和力。成功构建了重组真核表达载体pTT5-chCR2和pTT5-chC3 d;利用HEK 293F表达系统表达了His-chCR2和His-chC3 d蛋白;利用NI柱纯化系统纯化了His-chCR2和His-chC3 d蛋白;利用表面等离子共振技术,通过分析chCR2与chC3 d结合的动力学参数得出两者之间的平衡解离常数KD为1.37μmol/L。结果表明chCR2与其配体chC3 d之间的亲和力较高。