抗伪狂犬病毒闽A株单克隆抗体的制备及鉴定

用纯化的猪伪狂犬病病毒闽A株(PRV-FA)免疫Balb/c鼠,取脾细胞和骨髓瘤细胞进行细胞融合,经间接ELISA筛选,获得2株能稳定分泌伪狂犬病毒单克隆抗体的杂交瘤细胞株,分别命名为4G9E9和4H9C9.这2株杂交瘤细胞株细胞培养上清和小鼠腹水效价(EusA)分别为1:6 400、1:12 800及1:12 800、1:25 600.特异性鉴定结果表明,各株单抗均不与猪瘟病毒、乙脑病毒、猪呼吸繁殖障碍综合症病毒、猪细小病毒等发生交叉反应,这2株抗PRV杂交瘤细胞株的获得为进一步建立准确快速的抗原检测方法奠定了基础.     

猪繁殖与呼吸综合征病毒NSP9蛋白单克隆抗体制备与鉴定

根据NSP9非结构蛋白参数选取相应30 bp DNA片段,片段串联后人工合成并连接p ET-28a(+)载体,大肠杆菌BL21中表达得到可溶蛋白,镍柱亲和层析纯化后免疫Balb/c小鼠,取免疫鼠脾细胞与SP2/0细胞骨髓瘤细胞融合。细胞融合后利用间接ELISA法杂交瘤细胞阳性克隆筛选,2次亚克隆后共获得3株抗PRRSV NSP9单克隆抗体,命名为3B17、3J13、3F19。这3株单抗可与大肠杆菌表达的蛋白产物和PRRSV感染的Marc-145细胞发生特异性反应。在PRRSV感染的Marc-145细胞中,NSP9蛋白表达水平接毒后不断升高,48 h达最高,为深入研究NSP9功能奠定基础。     

犬2型腺病毒对犬的口服免疫研究

对未经犬腺病毒免疫的两月龄左右杂交狼犬，随机分为６组每组５只，另设５只对照，用作者分离鉴定和致弱驯化的犬喉气管炎病毒的第６０代ＤＫ细胞培养物（ＳＹＣＡＶ－２－ＤＫ６０）对犬进行免疫试验，在免疫第４周采血分离血清，测定犬腺病毒的血凝抑制抗体（ＨＩ）效价，结果３０只试验犬全部产生了ＨＩ抗体，表明ＳＹＣＡＶ－２可通过口服或饵料的途径对犬实施免疫，为犬口服联合疫苗以及犬２型腺病毒为勒体的口服狂犬 －腺病毒     

梅花鹿狂犬病的病原学研究

应用鼠脑传代,从具有神经症状和后躯麻痹的鹿尸脑组织分离获得5株弹状病毒。对其中一株——8202株,从形态学、生物学和理化学等方面进行了鉴定。应用弹状病毒料中狂犬病海和狂犬相关病毒等的抗血清或免疫腹水,在小鼠体内进行交叉中和试验,证明鹿毒8202株同狂犬病毒固定毒(北京株)有密切的抗原联系。经WHO狂犬病咨询和研究协作中心应用42个单克隆抗体,以间接荧光抗体试验检测鼠脑压印片,证明该株病毒的核衣壳抗原结构与大多数陆栖哺乳动物狂犬病毒相同,但有别于欧洲狐狸的狂犬病毒;用40个抗糖蛋白单克隆抗体,通过血清中和试验,证明8202株同欧洲狐狸毒株很相似。将该毒复旧鹿体,并接种犬及其他动物,发现其对鹿的毒力很强,而对犬及其他家畜毒力较弱,甚至没有毒力。结论认为,鹿毒8202株是狂犬病毒适应于鹿体的变异株。     

白菜型油菜黄籽遗传研究

利用4个白菜型油菜品种进行黄籽与非黄籽之间的正反交和相应的回交测交,以探讨其种皮颜色的遗传及其与其它性状的关系。结果表明:白菜型油菜种皮颜色具有母体效应。黄籽受两对重叠隐性基因控制。在黄籽与黑籽之间存在两对显隐性关系,而在黄籽与褐籽之间只存在一对显隐性关系。在相同遗传背景下,黄籽含油量比非黄籽要高,皮壳率要低,而千粒重差异不显著。     

禽流感病毒单克隆抗体的制备及其抗蛋白抗原的分析

以纯化的H9N2亚型禽流感病毒为抗原,免疫BALB/c小鼠,细胞融合后,经间接ELISA和血凝抑制试验(HI)筛选,获得了8株能稳定分泌抗禽流感病毒单克隆抗体的杂交瘤细胞株。特异性试验证明,8株杂交瘤细胞株诱生小鼠腹水的特异ELISA抗体效价可达1∶3.2×103~1∶5.1×106,其中2株HI效价达212。8株单抗与H5亚型血凝素分型抗原不发生血凝,与减蛋综合征(EDS-76)病毒、传染性支气管炎病毒(IBV)、新城疫病毒(NDV)均不反应。亚类鉴定证实,除1C7单抗为IgG2b外,其他7株均为IgG1亚类。Westernblotting试验分析初步表明,8株单抗至少针对纯化病毒粒子3种不同的蛋白抗原,其中3株针对核蛋白(NP),2株针对基质蛋白M1,2株针对血凝素HA/HA1。对感染细胞的Western blotting分析结果与纯化病毒结果基本一致,其中1株未明显沉淀纯化病毒粒子蛋白的单抗可以与感染细胞的M2蛋白多肽反应。     

卵黄抗体和金霉素对肉鸭小肠皱毛高度的影响

选用7日龄健康仙湖三号瘦肉型鸭240只，随机分为4组。对照组饲喂基础日粮，试验Ⅰ组添加50mg／l（g的金霉素，试验Ⅱ组添加1000mg／kg卵黄抗体，试验Ⅲ组添加50mg／kg的金霉素和1000mg／kg卵黄抗体。应用组织学技术和Tiger细胞图象分析系统，观测小肠绒毛高度的变化。结果表明：试验各组小肠段绒毛高度均比对照组有所提高，其中卵黄抗体组空肠段达到显著水平（P〈0．05）。     

Auto-antibodies specific for lymphocyte surface antigens—A review

Antilymphocyte auto-antibodies (ALAA) are defined as antibodies specific for lymphocyte surface determinants and produced in the absence of deliberate immunization with their specific antigen. ALAA are usually detected by the microlymphocytotoxicity test performed at 15°C, but they are also demonstrable by indirect immunofluorescence. ALAA are produced by genetically selected auto-immune mice (NZB, B/W, Swan). Defective thymus immunoregulatory function represents the main factor accounting for ALAA production. Conversely, ALAA may bind preferentially to subsets of T cells responsible for the suppression of various immune responses and therefore contribute to the deficiency of suppressor T cells in auto-immune mice.ALAA have been reported to occur in a wide variety of human diseases including auto-immune (systemic lupus erythematosus, rheumatoid arthritis), inflammatory or neoplastic diseases, viral bacterial or parasitic infections. Low titres of ALAA were reported in healthy aging subjects. Most of the antigens recognized by human ALAA are expressed on thymocytes, cord blood lymphocytes or peripheral T cells. Exceptional sera display a restricted specificity for a lymphocyte subset. Production of ALAA may result from immunization with cross-reacting antigens (microbial, parasitic or viral) or from alteration or unmasking of self-antigenic structures or it may represent a regulatory process of the immune response.     

Occurrence of antibodies to group specific chlamydia antigen in Finnish sheep, cattle and horse sera

A serological survey on the occurrence of group-specific chlamydial antibodies in random sera of Finnish sheep, cattle and horses was performed. The whole material consisted of 1347 serum samples, including 432 ovine, 454 bovine and 461 equine sera. The sera were sent to the laboratory for various serological tests during 1968–1972. Of the ovine sera 9.5%, bovine 12.8 % and equine 7.1 % showed a titer ≥ 1:16 in the complement fixation test.No definite geographic differences could be found in the distribution of the herds which showed positive results. The ubiquity of chlamydial infections in domestic mammals and their role as a cause of clinical diseases is discussed.     

Occurrence of Anti-Toxoplasma gondii Antibodies in Dogs in the Urban Area of Monte Negro,Rondônia,Brazil

The prevalence of anti-Toxoplasma gondii antibodies in dogs in an urban area of the municipality of Monte Negro, Rondônia, Brazil, was evaluated using an indirect immunofluorescent antibody test (IFAT). Blood samples were taken from 157 dogs living in 85 of the 94 blocks of the city. A seropositivity of 76.4% (120/157) was found and associations between the prevalence and the variables sex, age, type of raising and food were studied. The prevalence tended to increase with age (p<0.05); dogs over 24 months old had 85.5% (100/117) positivity, compared with 50% (20/40) in dogs less than 24 months old, showing postnatal exposure to the agent. It was also observed that dogs with access to the streets showed greater prevalence (84.9%) than companion animals (58.8%). There was no association between sex or the type of food (home-made or commercial) and anti-T. gondii antibodies.