Fetal Infection with Porcine Parvovirus in Herds with Reproductive Failure

During a 20 months period in 1978 and 1979 aborted, macerated and mummified fetuses as well as stillborn piglets from herds with reproductive failure were examined for evidence of infection with porcine parvovirus (PPV). A total of 602 cases were examined and evidence of infection with PPV was found in 269 (45 %). In 52 % of these antibody to PPV was found. Infective PPV as well as antibody to PPV were found in 41 %, whereas infective PPV alone was found in 7 %. When abortions were excluded from the results a high prevalence of infection with PPV (73–90 %) was found among fetuses of all sizes with the exception of fetuses dead late in gestation or at term.     

小鼠肝再生过程中脂质代谢相关通路中基因的表达谱变化

[目的]研究肝再生过程中肝脏内脂质代谢通路相关基因的表达谱变化。[方法]建立CC l4诱导肝再生小鼠模型,从小鼠肝组织中提取总RNA,通过微阵列基因芯片技术检测在不同肝再生期间脂质代谢通路相关基因的表达变化,并进行具体功能分析。[结果]肝再生过程中,有98个脂质代谢相关基因的表达水平发生了变化,根据这些基因表达的变化趋势可以分为8组。整体上看,基因表达前期表现为抑制,后期表现为上调。其中,脂肪酸的合成通路基因表达以上调为主,分解代谢通路变化并不明显;大多数胆汁酸合成通路基因在4.5天之前表现为抑制,在4.5天和7天时表现为上调。[结论]肝再生过程中,脂质代谢相关通路间的基因表达变化并不一致,这些数据为进一步研究脂质代谢相关通路对肝再生的调控作用提供了明确的基因范围。     

内毒素诱导的山羊红细胞膜和肝线粒体膜Ca~(2 )-ATP酶活性的改变及654-2的保护作用

通过静脉注射的方法研究了大肠杆菌内毒素（ＥＴ）对山羊红细胞膜和肝粒体膜损伤的膜Ｃａ２＋－ＡＴＰ酶活性的变化及６５４－２（山莨菪碱）的保护效应．结果表明,ＥＴ处理组（Ⅱ组）在１ｈ,３ｈ红细胞膜Ｃａ２＋－ＡＴＰ酶活性高于对照组（Ⅰ组）（ｐ＜００５）,５ｈ有下降趋势且持续到９ｈ．１２ｈ开始回升并低于Ⅰ组（ｐ＜００１,ｐ＜００５）．６５４－２处理组（Ⅲ组）,Ｃａ２＋－ＡＴＰ酶活性除在９ｈ、１２ｈ与Ⅰ组,在３ｈ与Ⅱ组差异不显著（ｐ＞００５）外,均高于Ⅰ组和Ⅱ组（ｐ＜００１,ｐ＜００５）．在肝线粒体中,Ⅱ组Ｃａ２＋－ＡＴＰ酶活性在５ｈ、１２ｈ均低于Ⅰ组和Ⅱ组（ｐ＜００５,ｐ＜００１）,Ⅲ组Ｃａ２＋－ＡＴＰ酶活性均高于Ⅰ组,但１２ｈ与Ⅰ组差异不显著（ｐ＞００５）．结果提示,ＥＴ具有诱导膜Ｃａ２＋－ＡＴＰ酶活性先激活后顿抑的效应,而６５４－２有明显保护膜Ｃａ２＋－ＡＴＰ酶活性的作用．     

附红细胞体病患畜病理组织学及血液学变化的研究

对急性附红细胞体病患畜（猪、犬）病理组织学、血液学和部分生理生化指标变化的研究显示:患畜均有体温升高、呼吸和心跳加快、谷-丙转氨酶和血清胆红素增高、血糖降低、红细胞压积、红细胞总数、淋巴细胞下降、白细胞总被上升等血液学和生理生化指标变化;病理剖检可见血液稀薄,血凝延迟、胃肠粘膜出血,其他部位粘膜、浆膜和脂肪黄染,肝脾肿大;心、肝、脾、肾等大部分实质器官有含铁血黄素沉着和广泛的器官损害等组织学变化。结果表明:贫血、肝功能下降、血糖降低和广泛的器官损害是急性附红细胞体病主要的特征。     

Effect of in utero exposure of Toddy (coconut palm wine) on liver function and lipid metabolism in rat fetuses

The objective of this study was to determine the effects of a country liquor Toddy (Coconut palm wine) and an equivalent quantity of ethanol on liver function and lipid metabolism in utero. Female albino rats with an average weight of 125± 5 g were exposed to Toddy from coconut palm (24.5 ml/kg-1 body weight/day) and ethanol (0.52 ml/kg body weight/day) for 15 days before conception and during pregnancy. On day 13 and day 19 of gestation, altered liver function and hyperlipidemia were seen in the fetuses of both the treated groups. Altered liver function was evidenced by the increased activity of alcohol dehydrogenase, aldehyde dehydrogenase, glutamic oxaloacetic transaminase (aspartate amino transferase (GOT)), glutamic pyruvic transaminase (alanine amino transferase (GPT)). Hyperlipidemia was caused by increased biosynthesis since the incorporation of 14C acetate into lipids and activities of HMG CoA reductase and lipogenic enzymes were elevated. Toddy treated fetuses were more severely affected than those exposed to an equivalent quantity of ethanol. Toddy seemed to potentiate the toxicity induced by alcohol suggesting the role of non alcoholic components. Hepatic functions of the day 13 fetuses were effected to a lesser degree than those in the day 19 hepatic liver.     

Restoration of exact design for partial compression perpendicular to the grain

Abstract

To correct the empirical design rules for locally loaded beams and blocks of Eurocode 5, the theoretical explanation of these rules and of the applied test data is given. It appears that the design rule may be far too conservative, or too unsafe in other cases, and only approximately applies for thin long bearing blocks and not for the short test specimens and support stresses of beams. The theory of the determining influence of load spreading possibility on the strength is verified by all other sufficient extended investigations leading to simple and reliable design rules.     

甲硝唑和替硝唑对幼犬肝功能的影响研究

[目的]为小动物临床用药提供参考。[方法]分别向刚断奶的幼犬腹腔注射1倍剂量、2倍剂量、5倍剂量的甲硝唑和替硝唑5d,并分别在用药前、停药后第1天、第7天测定试验犬的肝功能指标,探讨甲硝唑和替硝唑对犬肝功能的影响。[结果]2倍剂量的甲硝唑和替硝唑对幼犬肝功能的影响更为明显;大剂量的2种药物均可引起幼犬TRP增加、排黄色尿液等临床表现、肝静脉及肝窦充血、脂肪变性等病理学变化。[结论]替硝唑对幼犬的毒副作用低于甲硝唑。     

北美枫香主脉切断叶片的蒸腾冷却衰减和变色

蒸腾冷却被认为是植物的主要散热过程之一，而蒸腾冷却的衰减势必导致叶片温度的升高。本文用红外热像监测北美枫香叶片温度来研究其蒸腾冷却衰减现象。通过对北美枫香叶片局部区域的含水率、热像温度、气孔导度和RGB值进行微分研究，观察到了叶片主脉切断后的蒸腾衰减。用红外热像法观测到了北美枫香叶片主脉切断后明显的高温区。而且叶片的低含水率、较小的气孔导度和高叶温区域与叶色变红部位相吻合。表明持续的蒸腾冷却衰减诱发了远离水源的叶片局部胁迫区的保护性响应，且诱导断脉叶片的局部变成红或紫红色。通过对比研究发现，同样切断主脉的山茶花叶片则表现出截然不同的特征。