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21.
Chuozi Liang Zhongna Yu Guangming Zhu; Yixuan Li; Xueheng Sun; Hongning Jiang; Qijing Du; Rongbo Fan; Jun Wang; Yongxin Yang; Rongwei Han 《农业科学学报》2025
乳脂肪球膜(MFGM)结构复杂且具有多种功能;其组成受多种因素的影响。然而;目前关于整个泌乳期山羊乳脂肪球膜蛋白质组的系统研究很少。本研究共采集了来源于15只健康奶山羊的6个泌乳时间点的样品;采用数据独立采集(DIA)和数据依赖采集(DDA)两种方法;结合多元统计分析;对山羊MFGM蛋白质组进了分析。借助DIA方法;在整个泌乳期共鉴定出890种差异表达的MFGM蛋白。从1天到240天;嗜乳脂蛋白亚家族1成员A1、脂蛋白脂肪酶、围脂滴蛋白2、甘油三酯脂肪酶表达上调;而载脂蛋白E、补体C3、簇蛋白和免疫球蛋白则下调。此外;从1到90天;膜联蛋白A1、膜联蛋白A2、抗凝血酶III表达下调;然后到240天上调。蛋白-蛋白相互作用分析表明;白蛋白具有高度的关联性;因此被认为是中心蛋白。总之;我们的研究结果为了解整个泌乳期山羊MFGM蛋白的生物学特性提供了新的见解;这将有助于改进特定MFGM产品及婴儿配方奶粉的生产。 Abstract The milk fat globule membrane (MFGM) is a complex structure with numerous functions, and its composition is affected by a number of circumstances. There have been few systematic investigations on goat MFGM proteome profiling during lactation. Individual milk samples from 15 healthy dairy goats were obtained at six lactation time points for investigation of the MFGM proteome using both data-independent acquisition (DIA) and data-dependent acquisition (DDA) proteomics techniques combined with multivariate statistical analysis. Using the DIA method, 890 variably abundant MFGM proteins were discovered throughout the lactation cycle. From 1 to 240 d, butyrophilin subfamily 1 member A1, lipoprotein lipase, perilipin-2, and adipose triglyceride lipase were upregulated, while APOE, complement C3, clusterin, and IgG were downregulated. Furthermore, from 1 to 90 d, annexin A1, annexin A2, and antithrombin-III were downregulated, then upregulated by d 240. Albumin had a high degree of connectedness, indicating that it was a key protein, according to protein-protein interaction research. Overall, our findings gave new insights into the biological features of MFGM protein in goat milk throughout lactation, which may aid in the creation of specialized MFGM products and infant formula.
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吴宝珍 《农业图书情报学刊》2012,24(2):117-119
客观评价图书馆采编业务外包工作中代理机构的优势,及高校图书馆采编业务外包操作过程中遇到的实际问题,分析了高校图书馆现阶段采编业务外包的利弊及解决的方法。 相似文献
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泛在知识环境给高校图书馆建设与服务带来了一定的冲击,同时与带来了机遇,促使高校图书馆深入调研科研用户的需求,通过对科研用户信息行为的把握,更好地为用户服务。针对泛在知识环境下高校图书馆服务,笔者基于信息行为的研究,结合学科热点信息共享空间(IC),构建出虚拟知识共享空间,从理论方面介绍泛在知识环境、信息共享空间,从实践可能性分析信息行为研究主导下的虚拟知识共享空间服务对高校图书馆的价值。 相似文献
26.
引用政府补偿政策干预对企业与农户合作模式进行重复博弈分析。针对政府干预下的企业与农户行为特征,借用知识溢出模型得出相应的效用函数,并通过均衡分析,得出政府促进企业与农户合作的策略:第一,达成合作的策略需要保障贴现因子在特定的区间内波动;第二,对于政府补偿政策而言,需要增强农户与企业沼气能源使用率,减小博弈双方知识储备差异的,通过政府资金支持和技术支持增强沼气能源技术的追赶能力,同时合理的规划养殖企业和农户之间地理位置的距离等方式保证合作进行。 相似文献
27.
以KTA框架为原理,分析了农业科技人员知识转移的重要性,在此基础上提出了知识转移的具体路径,即从知识源、知识接受者和转移媒介3个方面对农业科技人员的知识转移提出建议。 相似文献
28.
Sander Janssen Ioannis N. AthanasiadisIrina Bezlepkina Rob KnapenHongtao Li Ignacio Pérez DomínguezAndrea Emilio Rizzoli Martin K. van Ittersum 《Computers and Electronics in Agriculture》2011,76(2):148-160
The ex-ante assessment of the likely impacts of policy changes and technological innovations on agriculture can provide insight into policy effects on land use and other resources and inform discussion on the desirability of such changes. Integrated assessment and modeling (IAM) is an approach that can be used for ex-ante assessment. It may combine several quantitative models representing different processes and scales into a framework for integrated assessment to allow for multi scale analysis of environmental, economic and social issues. IAM is a challenging task as models from different disciplines have a different representation of data, space and time. The aim of this paper is to describe our strategy to conceptually, semantically and technically integrate a chain of models from different domains to assess land use changes. The models that were linked are based on different modeling techniques (e.g. optimization, simulation, estimation) and operate on different time and spatial scales. The conceptual integration to ensure consistent linkage of simulated processes and scales required modelers representing the different models to clarify the data exchanged and interlinking of modeling methodologies across scales. For semantic integration, ontologies provided a way to rigorously define conceptualizations that can be easily shared between various disciplines. Finally, for technical integration, OpenMI was used and supplemented with the information from ontologies. In our case, explicitly tackling the challenge of semantic, conceptual and technical integration of models forced researchers to clarify the assumptions of their model interfaces, which helped to document the model linkage and to efficiently run models together. The linked models can now easily be used for integrated assessments of policy changes, technological innovations and societal and biophysical changes. 相似文献
29.
王稳琴 《农业图书情报学刊》2014,(9):12-15
随着高校图书馆用户信息需求变化趋势呈现学习时空泛在化、知识认知建构制和信息需求多样性特征,逐渐表现出与高校图书馆传统信息素养服务供给模式不匹配或错位,导致服务绩效低下。根据高校图书馆用户信息需求变化趋势特征,针对大学生日常生活和专业学习中的事实性知识获取需求,提出以用户为中心,利用虚拟嵌入平台和支架抛锚兼同级同侪互助教学策略的信息素养服务模式。 相似文献
30.
By convening a multidisciplinary team(the Monitoring Team) that included farmers,university and agency researchers, andnon-profit staff; a small group of farmers insoutheast Minnesota, U.S.A., bolstered thelegitimacy of the sustainable agriculturemovement. Through the experience of forming ateam and working with individuals who operatedwithin the mainstream knowledge paradigm,farmers gained validation of their knowledgeabout farming, while researchers came to valuealternative knowledge systems. In the contextof a socially embedded movement, farmers wereempowered by sharing their knowledge withresearchers, and ultimately contributed to thesustainable agriculture movement by challengingtraditional patterns of knowledge exchange. 相似文献