阿勒泰羊Fsp27基因5'UTR区g.16767667位点G>A突变与其尾脂沉积能力关联性分析

Fsp27基因在动物脂肪代谢过程中发挥着重要的调控作用。为了研究Fsp27基因5'UTR区g.16767667位点G>A突变与绵羊尾脂沉积能力的关联性,以尾脂沉积能力极强的脂尾(臀)型绵羊品种阿勒泰羊(Ovis aries)为研究对象,采用PCR-SSCP及测序技术研究了该SNP位点不同基因型在阿勒泰羊群体中的分布,并通过比较不同基因型个体尾形数据的差异,评估其与阿勒泰羊尾脂沉积能力的关联性。结果表明,在阿勒泰羊群体中Fsp27基因5'UTR区g.16767667位点G>A突变可以检测到GG、AG和AA三种基因型,基因型频率分别47.2%、38.4%和14.4%;卡方检验结果表明该位点在阿勒泰羊群体中处于Hardy-Weinberg平衡状态(P>0.05)。通过250只不同基因型阿勒泰羊尾形数据对比发现,GG和AG基因型个体脂尾(臀)的长、宽和厚3项数据均显著高于AA基因型个体(P<0.05),GG基因型个体亦略高于AG基因型个体,但差异不显著(P>0.05),推断G等位基因有利于阿勒泰羊尾脂沉积。     

青海省祁连县羊布鲁氏病发生情况调查及防治

羊布鲁氏杆菌病俗称布病,是由布鲁氏杆菌感染引起的一种人畜共患病。近年,我国家畜饲养量不断增加,动物及其产品流通不断频繁,部分地区布鲁氏杆菌病呈现上升趋势,严重威胁当地畜牧养殖产业和人民群众的身体健康。该文主要就祁连县羊布鲁氏杆菌病的发生情况进行调查,提出相应的防治对策。     

羊传染性胸膜肺炎防治

羊传染性胸膜肺炎是一种高度接触性传染性疾病,致病原是肺炎支原体。妊娠阶段的母羊受到病原侵染后表现为流产产下死胎,并表现出明显的繁殖障碍。近年,此病发生率逐年升高,造成的死亡现象不断增强,严重威胁养殖产业健康发展。作为养殖管理人员,应从该种疾病的流行病学、临床表现、病理变化和诊断方法等多角度入手,构建针对性的防治措施,严格落实预防为主的原则,为提高养殖效益奠定坚实的技术支撑。该文主要探讨羊传染性胸膜肺炎的防治过程。     

羊沙门氏菌病流行与防治

羊沙门氏菌病是是由多种致病性沙门氏菌感染引发的一种急性传染性疾病,不同年龄品种的羊都可以受到病原侵染,其中对羔羊造成的危害最为严重,尤其是断奶不久后的羔羊感染沙门氏菌后,表现的临床症状最为明显,致死率最高。临床上由于致病性沙门氏菌的种类较多,侵染途径多样,一旦受到病原侵染,将很难清理,会造成羊群反复流行,带来的经济损失不可估量。该文主要探讨羊沙门氏菌病的流行特点与防治措施。     

藏羊肝片吸虫病治疗措施

藏羊是青海地区农牧民群众养殖的特有羊品种,具有耐严寒、适应能力较强的特征。但在广大农牧地区,很多农民群众一直沿用散养养殖模式。整个羊群随着牧草的生长而迁徙,在放牧养殖过程中很容易受到多种寄生虫病的威胁,其中羊肝片吸虫病是威胁比较严重的一类人畜共患病。该文主要分析藏羊肝片吸虫病的流行病学、临床症状、病理变化、诊断方法和治疗措施。     

牛羊养殖中前胃疾病鉴别诊断方法

牛羊都是反刍动物,它们拥有4个胃,分别是瘤胃、网胃、瓣胃和真胃。前3个胃统称为前胃,在前胃中有大量的可以分解和消化饲料中粗纤维的细菌,是牛羊以粗饲料作为主要食物的原因之一。很多牛羊非常容易出现前胃疾病,饲养人员要掌握牛羊前胃疾病的预防办法和治疗措施。     

羊传染性脓疮皮炎治疗

羊传染性脓痂皮炎又被称为羊传染性脓疱病、羊口疮病,是由羊传染性脓疱病毒感染引发的一种急性接触性传染性疾病。羊养殖主产区均有发生,是一种常见疾病。羊传染性脓痂皮炎虽然造成的死亡率较低,但是会严重影响羊群正常采食,造成营养物质供给不足,身体逐渐消瘦,抵抗能力变差,易继发感染多种传染性疾病,表现出复杂的临床症状,威胁羊群健康。     

羊群四季放牧要点

羊具有适应性强、吸收和消化性能好、能抵御外界病原微生物的入侵,非常适合进行放牧饲养。通过合理放牧不仅可节省大量的饲草成本,还可将牧草中粗纤维、蛋白质和能量进行很好的消化和系统,满足机体生长发育的需要。但是在放牧中应根据季节的变换合理进行放牧活动,不仅促进机体膘情增长、适时增肥、发情和配种,提高羊群繁殖能力,同时还可根据牧场面积科学放牧,达到保护牧场草原的目的。该文主要论述羊群四季放牧的要点。     

Production and Identification of Clone Sheep Using Bone Marrow Mesenchymal Stem Cells as Donor Cell

In order to obtain the cloned sheep using bone marrow mesenchymal stem cells（BMSCs）as the donor cells,the BMSCs of sheep were chosen and reconstructed embryos were built to transfer.10 published microsatellite markers were chosen,and the DNA samples from clone sheep,donor cells and surrogate ewes were amplified,and the relationship of father-son（RCP）was analyzed using the Quantity One for genotyping.The results showed that the reconstructed embryos were successfully built for electric fusion using sheep BMSCs as nuclear donor,and making nuclear transplantation into enucleated mature oocytes of which the fusion rate was 80.62%.20 surrogate ewe were chosen to be implanted with the reconstructed embryos at morula stage by implant surgery,and 5 lambs were born and only 3 were survived.The genotype of cloned sheep was in line with the dornor cell and the RCP were more than 99.999%.In conclusion,the first clone sheep were obtained successfully by using BMSCs as a nuclear donor in this experiment.     

Effect of Aerosol OT and Paraformaldehyde Supplement on the Voluntary Intake,Digestion and Metabolism of Late-pregnant Ewe Fed with Ground Diet

The Small-tail Han ewes with 3 years old, (48.7±2.0)kg body weight, carring the multi-lamb gene, were fed with the same diet, mating after one month.18 pregnant ewes were selected and divided into 3 groups(groups 1(control group), 2 and 3).From 90 days of pregnancy, groups 2 and 3 were supplemented with aerosol OT(800 mg/kg) and paraformaldehyde(300 mg/kg), respectively, and the experiments of digestion and metabolism were carried on from 121 to 130 days of pregnancy to study the effect of aerosol OT and paraformaldehyde supplements on the voluntary intake, digestion and metabolism, lambing of late-pregnant ewes.The results showed that when fed with aerosol OT, the voluntary intake of dry matter was increased by 13.0%(P<0.01);The amount of digestion of DM, OM, CP, cellulose and GE was increased by 12.4%(P<0.05), 14.4%(P<0.01), 23.2%(P<0.01), 15.2%(P>0.05) and 27.4%(P<0.01), respectively;The retention of N, Ca and P was increased by 31.1%(P<0.01), 48.5%(P<0.01) and 31.9%(P<0.01), respectively;The serum triglyceride and free fatty acid content tended to decrease;but the apparent digestibility of the dietary nutrients were not significantly affected(P>0.05);The loss of ewe postpartum weight was reduced by 61.9%(P<0.01), a nest of lamb weight was increased by 4.2%(P>0.05).In the condition of feeding paraformaldehyde, the voluntary intake of dry matter was increased by 12.6%(P<0.01);The amount of digestion of DM, OM, CP, cellulose and GE was increased by 15.4%(P<0.01), 16.2%(P<0.01), 22.4%(P<0.01), 14.2%(P>0.05) and 29.0%(P<0.01), respectively;The retention of N, Ca and P was increased by 33.5%(P<0.01), 48.9%(P<0.01) and 36.3%(P<0.01), respectively;But the apparent digestibility of the dietary nutrients were no significantly affected(P>0.05);The serum β-hydroxybutrate and free fatty acid content tended to decrease;The loss of ewe postpartum weight was reduced by 57.1%(P<0.01), a nest of lamb weight was increased by 11.1%(P<0.05).This study showed that by feeding aerosol OT or paraformaldehyde, the intake, digestibility of Ca, P and N could be improved in late-pregnant ewe, the body weight loss, body fat mobilization could be reduced, and the birth weight of litter could be increased.