甲硝唑对鸡离体外周血淋巴细胞的作用

对咪唑类化合物甲硝唑对离体鸡外周血淋巴细胞(PBL)增殖及伴刀豆球蛋白A(ConA)诱导细胞(CIC)活性的作用并与咪唑类化合物左旋咪唑进行了比较；甲硝唑和左旋咪唑均以剂量依赖性方式促进鸡PBL的增殖；甲硝唑和左旋咪唑与ConA对促进鸡PBL增殖均呈现协同作用；甲硝唑和左旋咪唑对脂多糖(LPS)激活鸡PBL均具有明显促增殖作用；甲硝唑和左旋咪唑均可逆转磷酸组胺和肾上肾对ConA和nLPS激活鸡PBL增殖的抑制作用；高浓度左旋咪唑(100～400μg／mL)可明显逆转氢化可的松对ConA激活鸡PBL增殖的抑制作用，各种浓度甲硝唑和左旋咪唑均可明显逆转氢化可的松对LPS激活鸡PBL增殖的抑制作用；甲硝唑和左旋咪唑均可促进CD^ 4细胞增殖，抑制CE^ 8细胞增殖，使CD^ 4／CD^ 8细胞比值升高，CIC活性增强。     

Sarcocystis neurona-specific immunoglobulin G in the serum and cerebrospinal fluid of horses administered S neurona vaccine

A vaccine against Sarcocystis neurona, which induces equine protozoal myeloencephalitis (EPM), has received conditional licensure in the United States. A major concern is whether the immunoglobulin G (IgG) response elicited by the vaccine will compromise the use of Western blotting (WB) as a diagnostic tool in vaccinated horses with neurologic disease. Our goals were to determine if vaccination (1) causes seroconversion: (2) causes at least a transient increase in S neurona-specific IgG in the cerebrospinal fluid (CSF); and (3) induces an IgG response that can be differentiated from that induced by natural exposure. Horses included in the study (n = 29) were older than 6 months with no evidence of neurologic disease. The presence or absence of anti-S neurona antibodies in the serum of each horse was determined by WB analysis. Seropositive horses had CSF collected and submitted for cytology, CSF index, and WB analysis. The vaccine was administered to all the horses and boostered 3-4 weeks later. On day 14 after the 2nd administration, serum and CSF were collected and analyzed. Eighty-nine percent (8 of 9) of the initial seronegative horses seroconverted after vaccination, of which 57% (4 of 7) had anti-S neurona IgG in their CSE Eighty percent (16 of 20) of the seropositive horses had an increase in serum S neurona IgG after vaccination. Of the 6 of 20 horses that were initially seropositive/CSF negative, 2 were borderline positive for anti-S neurona IgG in the CSF, 2 tested positive, and 2 were excluded because the CSF sample had been contaminated by blood. There were no WB banding patterns that distinguished samples from horses that seroconverted due to vaccination versus natural exposure. Caution must be used in interpreting WB analysis from neurologic horses that have been recently vaccinated for EPM.     

免疫学课程实践性知识的建构

针对实践性知识的特征,免疫学课程从获得经验,进行反思性总结和评价两大方面进行改革.认为教学中应注重与中学教学实际的联系,应用建构主义学习理论,采用问题讨论法、案例教学法、研究性学习、参与式学习法,以及增加实践机会等多种教学形式,采用综合评价体系,促进学生实践性知识的构建.     

Equine Herpesvirus-1 Consensus Statement

Equine herpesvirus-1 is a highly prevalent and frequently pathogenic infection of equids. The most serious clinical consequences of infection are abortion and equine herpesvirus myeloencephalopathy (EHM). In recent years, there has been an apparent increase in the incidence of EHM in North America, with serious consequences for horses and the horse industry. This consensus statement draws together current knowledge in the areas of pathogenesis, strain variation, epidemiology, diagnostic testing, vaccination, outbreak prevention and control, and treatment.     

隐孢子虫病诊断研究进展

本文对隐孢子虫病中几种常见的诊断方法进行了分类、总结;对其操作过程及应用情况做了简要的叙述;并对方法间优缺点进行了初步分析.     

“食品免疫学”课程教学改革与探索

"食品免疫学"作为食品专业的一门基础课程,内容繁多而抽象,理解及记忆难度大,学生在学习过程中缺乏学习热情。针对该课程目前存在的问题,提出了合理的意见,通过教学内容的调整、教学方式的改进,注重考核过程等方式对该课程进行改革与探索,以期学生能提高学习的积极与主动性,提高教学效果与质量,培养出理论丰富、技能扎实的食品专业人才。